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    Chemical modifications to polymer surfaces and the application of polymer grafting to biomaterials
    1.
    发明专利
    Chemical modifications to polymer surfaces and the application of polymer grafting to biomaterials 未知

    公开(公告)号：AU2003269873A8

    公开(公告)日：2003-12-19

    申请号：AU2003269873

    申请日：2003-05-13

    Applicant: UNIV CALIFORNIA

    Inventor： LI GUANN-PYNG ,  SIMS CHRISTOPHER E ,  REN XUEGIN ,  HU SHUWEN ,  BACHMAN MARK ,  ALLBRITTON NANCY

    IPC: C08J7/18 ,  G01N27/447 ,  G02B1/04 ,  G02C7/04 ,  C08J7/04

    Abstract: Polymer-based biomaterials are popular due to ease of fabrication and low costs. However, many polymer substrates have undesirable surface properties. The invention provides a procedure to covalently apply a graft polymer to the surface of a polymer substrate by ultraviolet graft polymerization. The graft polymer is formed from monomers such as PEG, AA, monomethoxy acrylate PEG, HEMA, or DMA. Also, mixed monomers may be used to create the graft and the surface properties of the graft may be tailored for different properties, including hydrophobicity, friction coefficient, electroosmotic mobilities and electrophoretic separations. The invention has particular utility in tailoring surface chemistries in ocular lenses and polymer microdevices. I. II. R: -OH Acrylic Acid(AA) -NH 2 Acrylamide (AM) -N(CH 3 ) 2 Dimethylacrylamide (DMA) -OCH 2 CH 2 OH 2-Hydroxyethylacrylate (HEA) -O(CH 2 CH 2 O) n CH 3 PEG monomethyoxylacrylate (PEG)

    Method and apparatus for detecting enzymatic activity using molecules that change electrophoretic mobility
    4.
    发明专利
    Method and apparatus for detecting enzymatic activity using molecules that change electrophoretic mobility 未知

    公开(公告)号：AU6232000A

    公开(公告)日：2001-02-13

    申请号：AU6232000

    申请日：2000-07-21

    Applicant: UNIV CALIFORNIA

    Inventor： ALLBRITTON NANCY L ,  SIMS CHRISTOPHER E ,  BERNS MICHAEL W ,  MEREDITH GAVIN D ,  KRASIEVA TATIANA B ,  TROMBERG BRUCE J

    IPC: G01N27/447 ,  C12M1/00 ,  C12M1/33 ,  C12N13/00 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/48 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50

    Abstract: The activity of intracellular chemical reactions of molecules is measured by the use of fluorescently labeled substrate molecules that undergo a change in electrophoretic mobility upon chemical reaction such as that catalyzed by an enzyme. Specificity is achieved by using labeled substrate molecules that can be acted upon only by specific enzymes. Thus the activity of a specific enzyme or class of enzymes can be determined. Measurements are made with the intracellular presence of such substrate molecules, at some time of interest, typically after exposure of the cell to a stimulus that activates a particular enzymatic pathway. To ensure accuracy, measurements must be made in a timely manner so as to minimize chemical reactions occurring subsequent to the time of interest. Fast controllable laser lysis is used to obtain the contents of a single cell into which reporter substrate molecules have been introduced. The cell contents are then subjected to capillary electrophoresis and enzymatic activity is determined by comparing amounts of substrate molecules to amounts of enzymatically altered substrate molecules which are separated by the electrophoresis and identified by the presence of a fluorescent label.

    CHEMICAL MODIFICATIONS TO POLYMER SURFACES AND THE APPLICATION OF POLYMER GRAFTING TO BIOMATERIALS
    5.
    发明专利
    CHEMICAL MODIFICATIONS TO POLYMER SURFACES AND THE APPLICATION OF POLYMER GRAFTING TO BIOMATERIALS 未知

    公开(公告)号：AU2003269873A1

    公开(公告)日：2003-12-19

    申请号：AU2003269873

    申请日：2003-05-13

    Applicant: UNIV CALIFORNIA

    Inventor： ALLBRITTON NANCY ,  SIMS CHRISTOPHER E ,  HU SHUWEN ,  REN XUEGIN ,  LI GUANN-PYNG ,  BACHMAN MARK

    IPC: C08J7/18 ,  G01N27/447 ,  G02B1/04 ,  G02C7/04

    Abstract: Polymer-based biomaterials are popular due to ease of fabrication and low costs. However, many polymer substrates have undesirable surface properties. The invention provides a procedure to covalently apply a graft polymer to the surface of a polymer substrate by ultraviolet graft polymerization. The graft polymer is formed from monomers such as PEG, AA, monomethoxy acrylate PEG, HEMA, or DMA. Also, mixed monomers may be used to create the graft and the surface properties of the graft may be tailored for different properties, including hydrophobicity, friction coefficient, electroosmotic mobilities and electrophoretic separations. The invention has particular utility in tailoring surface chemistries in ocular lenses and polymer microdevices. I. II. R: -OH Acrylic Acid(AA) -NH 2 Acrylamide (AM) -N(CH 3 ) 2 Dimethylacrylamide (DMA) -OCH 2 CH 2 OH 2-Hydroxyethylacrylate (HEA) -O(CH 2 CH 2 O) n CH 3 PEG monomethyoxylacrylate (PEG)

    Fast controllable laser lysis of cells for analysis
    6.
    发明专利
    Fast controllable laser lysis of cells for analysis 未知

    公开(公告)号：AU2889199A

    公开(公告)日：1999-09-20

    申请号：AU2889199

    申请日：1999-03-03

    Applicant: UNIV CALIFORNIA

    Inventor： ALLBRITTON NANCY L ,  SIMS CHRISTOPHER E ,  BERNS MICHAEL W ,  MEREDITH GAVIN D ,  KRASIEVA TATIANA B ,  TROMBERG BRUCE J

    IPC: G01N33/48 ,  C12M1/33 ,  C12N13/00 ,  G01N27/447 ,  G01N33/483 ,  G01N27/26

    Abstract: Fast lysis of a single cell or cellular component thereof is performed by generating a shock wave in a medium in which the cell or cellular component thereof is positioned. The cell or cellular component thereof is either positioned by laser tweezers or cultured as an adhered cell or cellular component thereof to minimize manipulation trauma. The disclosed method completely lyses a single cell or cellular component thereof in a controllable manner in milliseconds or less followed immediately by the loading of the cellular contents into a capillary for analyte separation and detection. The cell or cellular component thereof is adjacent the inlet of an electrophoretic column through which a gravity siphon flow of the medium is maintained. The lysed contents of the cell or cellular component thereof enter the electrophoretic column in less than 33 msec, are separated and analyzed by laser induced fluorescence. The method takes advantage of the shock wave produced by a highly focused laser pulse which is created in a medium adjacent to the cell or cellular component thereof. In the illustrated embodiment the laser pulse is focused in the glass substrate at or near a glass-to-buffer interface of a cell chamber in which the cell or cellular component thereof to be lysed has been cultured.

    SYSTEMS FOR COLLECTION OF SINGLE CELLS AND COLONIES
    8.
    发明申请
    SYSTEMS FOR COLLECTION OF SINGLE CELLS AND COLONIES 审中-公开
    用于收集单细胞和菌落的系统

    公开(公告)号：WO2007118208A3

    公开(公告)日：2008-11-06

    申请号：PCT/US2007066171

    申请日：2007-04-06

    Applicant: UNIV CALIFORNIA ,  ALLBRITTON NANCY ,  SIMS CHRISTOPHER E ,  WANG YULI ,  BACHMAN MARK ,  LI GUANN-PYNG ,  STANBRIDGE ERIC

    Inventor： ALLBRITTON NANCY ,  SIMS CHRISTOPHER E ,  WANG YULI ,  BACHMAN MARK ,  LI GUANN-PYNG ,  STANBRIDGE ERIC

    IPC: C12Q1/24

    CPC classification number: G01N1/286 ,  B01L3/5085 ,  B01L2200/0668 ,  B01L2300/0829 ,  C12M23/12 ,  C12M47/04 ,  G01N2001/2886

    Abstract: A plate manufactured to enable samples of cells, micro-organisms, proteins, DNA, biomolecules and other biological media to be positioned at specific locations or sites on the plate for the purpose of performing addressable analyses on the samples. Preferably, some or all of the sites are built from a removable material or as pallets so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis. The plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses. Use of the plate advantageously enables the selection and sorting of cells based on dynamic phenomena and the rapid establishment of stable tranfectants.

    Abstract translation: 制造的板可使细胞，微生物，蛋白质，DNA，生物分子和其他生物培养基的样品定位在板上的特定位置或位点，以便对样品进行可寻址分析。 优选地，一些或所有的部位由可移除的材料或托盘构成，使得感兴趣的样品的子集可以容易地与板分离以用于进一步的处理或分析。 该板可以包含增强或促进样品的附着和/或功能的结构或化学处理，并且促进或辅助其分析。 板的使用有利于基于动态现象和快速建立稳定的转染剂来选择和分选细胞。

    A MICRO-BUBBLE PLATE FOR PATTERNING BIOLOGICAL AND NON-BIOLOGICAL MATERIALS
    10.
    发明专利
    A MICRO-BUBBLE PLATE FOR PATTERNING BIOLOGICAL AND NON-BIOLOGICAL MATERIALS 未知

    公开(公告)号：CA2625071C

    公开(公告)日：2017-04-18

    申请号：CA2625071

    申请日：2006-10-10

    Applicant: UNIV CALIFORNIA

    Inventor： WANG YULI ,  BACHMAN MARK ,  SIMS CHRISTOPHER E ,  LI GUANN-PYNG ,  ALLBRITTON NANCY

    IPC: C12M1/22 ,  B01L3/00

    Abstract: Systems and methods for patterning biological and non-biological material at specific sites on a plate, as well as growing three dimensional structures. Preferred embodiments comprise a plate (6) with regions (16) that will trap gas, usually in the form of bubbles (10), when the plate (6) is submerged in liquid. Other embodiments of the present invention include a method for placing materials on the plate (6) at predetermined locations through the use of trapped gas to prevent materials from collecting at unwante regions. The plate (6) has great utility for plating cells and tissues (5) at specific sites, such as on an array. The disclosed method can also be used to coat the surface of a plate (6) at specific locations for patterned coating applications and to build up materials to produce three dimensional structures, including micromechanical structures where the structures may be formed from living or nonliving material, organic or inorganic, and the like.

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