公开(公告)号：CA2483697C

公开(公告)日：2010-03-30

申请号：CA2483697

申请日：2003-05-07

Applicant: UNIV NORTHWESTERN

Inventor： MIRKIN CHAD ,  CAO YUN-WEI ,  JIN RONGCHAO

IPC: C12Q1/68 ,  G01N21/65 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/02 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553 ,  G01N33/566 ,  G01N33/58

Abstract: The invention encompasses reagents comprising particles with at least one Raman dye and a specific binding members bound thereto and methods of using such reagents. The invention also encompasses reagents of a specific binding member and two or more different Raman dyes and methods for using such reagents. New types of particle probes having a specific binding member bound thereto are described. These reagents are used in a novel detection strategy that utilizes the catalytic properties of the Au nanoparticles to generate a silver coating that can behave as a surface-enhanced Raman scattering (SERS) promoter for the dye-labeled particles that have been captured by target and an underlying chip in microarray format. The strategy provides the high sensitivity and high selectivity attributes of grey-scale scanometric detection but provides a route to multiplexing and ratioing capabilities since a very large number of probes can be designed based upon the concept of using a Raman tag as a spectroscopic fingerprint in detection. These spectra are used as fingerprints to differentiate oligonucleotide or other targets in one solution. This method has been used to distinguish six dissimilar DNA targets with six Raman labeled nanoparticle probes, and also two RNA targets with single nucleotide polymorphisms (SNPs).

公开(公告)号：CA2654973A1

公开(公告)日：2008-07-31

申请号：CA2654973

申请日：2007-06-28

Applicant: UNIV NORTHWESTERN

Inventor： SALAITA KHALID ,  MIRKIN CHAD

IPC: G03F7/00

Abstract: Lithographic and nanolithographic methods that involve patterning a first compound on a substrate surface, exposing non-patterned areas of the substr ate surface to a second compound and removing the first compound while leavi ng the second compound intact. The resulting hole patterns can be used as te mplates for either chemical etching of the patterned area of the substrate o r metal deposition on the patterned area of the substrate.

公开(公告)号：AU2007211939A1

公开(公告)日：2007-09-13

申请号：AU2007211939

申请日：2007-08-24

Applicant: UNIV NORTHWESTERN

Inventor： CAO YUN-WEI ,  RONGCHAO JIN ,  MIRKIN CHAD

IPC: G01N21/65 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553 ,  G01N33/566 ,  G01N33/58

公开(公告)号：AU2003237180B2

公开(公告)日：2007-05-24

申请号：AU2003237180

申请日：2003-05-07

Applicant: UNIV NORTHWESTERN

Inventor： JIN RONGCHAO ,  MIRKIN CHAD ,  CAO YUN-WEI

IPC: G01N21/65 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553 ,  G01N33/566 ,  G01N33/58

Abstract: The invention encompasses reagents comprising particles with at least one Raman dye and a specific binding members bound thereto and methods of using such reagents. The invention also encompasses reagents of a specific binding member and two or more different Raman dyes and methods for using such reagents. New types of particle probes having a specific binding member bound thereto are described. These reagents are used in a novel detection strategy that utilizes the catalytic properties of the Au nanoparticles to generate a silver coating that can behave as a surface-enhanced Raman scattering (SERS) promoter for the dye-labeled particles that have been captured by target and an underlying chip in microarray format. The strategy provides the high sensitivity and high selectivity attributes of grey-scale scanometric detection but provides a route to multiplexing and ratioing capabilities since a very large number of probes can be designed based upon the concept of using a Raman tag as a spectroscopic fingerprint in detection. These spectra are used as fingerprints to differentiate oligonucleotide or other targets in one solution. This method has been used to distinguish six dissimilar DNA targets with six Raman labeled nanoparticle probes, and also two RNA targets with single nucleotide polymorphisms (SNPs).

公开(公告)号：AU2003247904A8

公开(公告)日：2004-01-23

申请号：AU2003247904

申请日：2003-07-07

Applicant: UNIV NORTHWESTERN

Inventor： SALAITA KHALID ,  MIRKIN CHAD ,  ZHANG YI

IPC: B01L3/00 ,  C40B60/14 ,  C25F1/00 ,  C23C22/82

Abstract: The invention provides a simple and convenient strategy for reducing the dimensions of organic micro- and nanostructures on metal surfaces. By varying electrochemical desorption conditions, organic structures patterned by Dip-Pen Nanolithography or any of the micro-contact printing procedures can be gradually desorbed in a controlled fashion. The electrochemical desorption is initiated at the exterior of the feature and moves inward as a function of time. The desorption process and adsorbate desorption are modified and controlled as a function of substrate morphology, adsorbate head and tail groups, and electrolyte solvent and salt. Different nanostructures made of different adsorbates can be miniaturized based upon judicious selection of adsorbate and supporting electrolyte.

公开(公告)号：AU2003247904A1

公开(公告)日：2004-01-23

申请号：AU2003247904

申请日：2003-07-07

Applicant: UNIV NORTHWESTERN

Inventor： SALAITA KHALID ,  MIRKIN CHAD ,  ZHANG YI

IPC: B01L3/00 ,  C40B60/14

Abstract: The invention provides a simple and convenient strategy for reducing the dimensions of organic micro- and nanostructures on metal surfaces. By varying electrochemical desorption conditions, organic structures patterned by Dip-Pen Nanolithography or any of the micro-contact printing procedures can be gradually desorbed in a controlled fashion. The electrochemical desorption is initiated at the exterior of the feature and moves inward as a function of time. The desorption process and adsorbate desorption are modified and controlled as a function of substrate morphology, adsorbate head and tail groups, and electrolyte solvent and salt. Different nanostructures made of different adsorbates can be miniaturized based upon judicious selection of adsorbate and supporting electrolyte.

公开(公告)号：AU2003237180A1

公开(公告)日：2003-11-11

申请号：AU2003237180

申请日：2003-05-07

Applicant: UNIV NORTHWESTERN

Inventor： MIRKIN CHAD ,  CAO YUN-WEI ,  JIN RONGCHAO

IPC: G01N21/65 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553 ,  G01N33/566 ,  G01N33/58

Abstract: The invention encompasses reagents comprising particles with at least one Raman dye and a specific binding members bound thereto and methods of using such reagents. The invention also encompasses reagents of a specific binding member and two or more different Raman dyes and methods for using such reagents. New types of particle probes having a specific binding member bound thereto are described. These reagents are used in a novel detection strategy that utilizes the catalytic properties of the Au nanoparticles to generate a silver coating that can behave as a surface-enhanced Raman scattering (SERS) promoter for the dye-labeled particles that have been captured by target and an underlying chip in microarray format. The strategy provides the high sensitivity and high selectivity attributes of grey-scale scanometric detection but provides a route to multiplexing and ratioing capabilities since a very large number of probes can be designed based upon the concept of using a Raman tag as a spectroscopic fingerprint in detection. These spectra are used as fingerprints to differentiate oligonucleotide or other targets in one solution. This method has been used to distinguish six dissimilar DNA targets with six Raman labeled nanoparticle probes, and also two RNA targets with single nucleotide polymorphisms (SNPs).

公开(公告)号：AT515698T

公开(公告)日：2011-07-15

申请号：AT03736550

申请日：2003-05-07

Applicant: UNIV NORTHWESTERN

Inventor： MIRKIN CHAD ,  CAO YUN-WEI ,  JIN RONGCHAO

IPC: G01N21/65 ,  G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/553 ,  G01N33/566 ,  G01N33/58

Abstract: The invention encompasses reagents comprising particles with at least one Raman dye and a specific binding members bound thereto and methods of using such reagents. The invention also encompasses reagents of a specific binding member and two or more different Raman dyes and methods for using such reagents. New types of particle probes having a specific binding member bound thereto are described. These reagents are used in a novel detection strategy that utilizes the catalytic properties of the Au nanoparticles to generate a silver coating that can behave as a surface-enhanced Raman scattering (SERS) promoter for the dye-labeled particles that have been captured by target and an underlying chip in microarray format. The strategy provides the high sensitivity and high selectivity attributes of grey-scale scanometric detection but provides a route to multiplexing and ratioing capabilities since a very large number of probes can be designed based upon the concept of using a Raman tag as a spectroscopic fingerprint in detection. These spectra are used as fingerprints to differentiate oligonucleotide or other targets in one solution. This method has been used to distinguish six dissimilar DNA targets with six Raman labeled nanoparticle probes, and also two RNA targets with single nucleotide polymorphisms (SNPs).

公开(公告)号：AT502299T

公开(公告)日：2011-04-15

申请号：AT06851430

申请日：2006-08-18

Applicant: UNIV NORTHWESTERN

Inventor： MIRKIN CHAD ,  VEGA RAFAEL ,  MASPOCH DANIEL ,  SALAITA KHALID

IPC: G01N33/50

Abstract: A nanoarray template utilizing coordination chemistry or metal ion binding to control the site-isolation and orientation of virus particles is provided. The nanoarray template is generated by lithography including Dip Pen Nanolithography. The surface chemistry that is inherent in many viruses, metal-ion based or inorganic coordination chemistry is used to immobilize individual virus particles without the need for their genetic modification. Single particle control enables a wide variety of studies involving viruses that are not possible with microarrays, including single particle, single cell infectivity studies, exploration of such structures as templates in materials synthesis and molecular electronics, and studies aimed at understanding how surface presentation influences their bioactivity. This is an example of such control at the single-particle level, and therefore, commercial use of nanoarrays in biological systems.

公开(公告)号：ES2356600T3

公开(公告)日：2011-04-11

申请号：ES00911560

申请日：2000-01-07

Applicant: UNIV NORTHWESTERN

Inventor： MIRKIN CHAD ,  PINER RICHARD ,  HONG SEUNGHUM

IPC: H01J49/00 ,  B82B3/00 ,  G01B7/34 ,  G03F7/00

Abstract: Un método de nanolitografía que comprende: proporcionar un sustrato; proporcionar una punta de microscopio como sonda de barrido; recubrir la punta con un compuesto de patronamiento; y poner en contacto la punta recubierta con el sustrato de manera que se aplique el compuesto al sustrato con la ayuda de un medio de transporte, en donde el medio de transporte forma un menisco que cierra la brecha entre la punta y el sustrato de manera que el compuesto de patronamiento sea transportado hasta el sustrato por medio de transporte capilar, con el fin de producir un patrón deseado.