Abstract:
Solid supports and ligands are provided for purification of biomolecules by mixed-mode anion exchange-hydrophobic chromatography. Compositions can have the formula Support-(X)—N(R1, R2)—R3-L-Ar, or a salt thereof, wherein: Support is a chromatographic solid support; X is a spacer or absent; R1 and R2 are each selected from hydrogen and an alkyl comprising 1-6 carbons; R3 is an alkyl comprising 1-6 carbons or a cyclo alkyl comprising 1-6 carbons; L is NR4, O, or S; wherein R4 is hydrogen or an alkyl comprising 1-6 carbons; and Ar is an aryl. Methods are also provided for using solid supports and ligands to purify biomolecules such as monomeric antibodies.
Abstract:
Treatment of anion exchange materials containing hydroxyl containing moieties in the beta position relative to the quaternary center in the hydroxide form with glycidol substantially alters the selectivity of the anion exchange material. Furthermore, sequential treatments of first a hydroxide containing solution to put the anion exchange material in the hydroxide form followed by treatment with glycidol in an alternating sequence progressively changes selectivity in a predictable manner allowing facile manipulation of selectivity. Unique to the selectivities achievable with this chemistry is the ability to reverse the elution order of sulfate and carbonate. With all other known systems, carbonate elutes ahead of sulfate and sometimes compromises the ability to quantitate sulfate. With glycidol treatment, carbonate can be moved after sulfate which eliminates interference issues for samples containing significantly more carbonate than sulfate. This modification is useful for columns operated with a hydroxide eluent system as well as columns operated with a carbonate eluent system.
Abstract:
Anion exchange polymers comprise a plurality of repeating units of formula (I). The polymer may be synthesized from a super acid catalyzed polyhydroxyalkylation reaction of monomers Ar1′, Ar2′, and X1′ to form a neutral precursor polymer followed by a Menshutkin reaction to convert the neutral precursor polymer to the anion exchange polymer.
Anion exchange membranes and membrane electrode assemblies incorporating the anion exchange polymers are also described.
Abstract:
The present invention provides a capsule-type compound consisting of: a capsule-type divalent cation consisting of a capsule framework represented by the following formula (1) and a sulfate ion (SO42−) encapsulated in the capsule framework; and a sulfate ion (SO42−) as a counter ion to the capsule-type divalent cation. In the formula (1), R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11, R12, and R13 each independently represent a hydrogen atom or a methyl group; and M1 and M2 each independently represent Cu2+, Fe2+, Ni2+, Co2+, or Zn2+.
Abstract:
A humic product, such as ammonium humus crystallate or crystal humus acid is treated with an acid to esterify part of the hydroxyl groups, which are then etherified with the nonesterified hydroxyl groups. The resulting insolubilized humic material is amidated with an excess of a polyamine to produce an anion exchanger.