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    METHOD AND APPARATUS FOR COMBINATION OF LOCALIZATION MICROSCOPY AND STRUCTURED ILLUMINATION MICROSCOPY
    211.
    发明申请
    METHOD AND APPARATUS FOR COMBINATION OF LOCALIZATION MICROSCOPY AND STRUCTURED ILLUMINATION MICROSCOPY 审中-公开
    本地化显微镜和结构化照明显微镜的组合方法与装置

    公开(公告)号：WO2014180566A1

    公开(公告)日：2014-11-13

    申请号：PCT/EP2014/001228

    申请日：2014-05-07

    Applicant: RUPRECHT-KARLS-UNIVERSITÄT HEIDELBERG

    Inventor： BEST, Gerrit ,  CREMER, Christoph ,  ROSSBERGER, Sabrina ,  DITHMAR, Stefan

    IPC: G02B21/00 ,  G01N21/64 ,  G02B21/16 ,  G02B21/36 ,  G02B27/58

    CPC classification number: G02B21/367 ,  G01N21/6445 ,  G01N21/6458 ,  G01N2201/0675 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/361 ,  G02B27/58

    Abstract: The invention relates to a fluorescent microscope and a respective method for obtaining super-resolution images of a sample labelled with at least one type fluorescent label by combining localization microscopy and structured illumination microscopy. In an aspect, the fluorescent microscope comprises one or more light sources and an illumination system having a structured illumination path, in which a pattern generation system is positioned, for illuminating the sample with structured illumination light and a localization illumination path for illuminating the sample with localization illumination light. A switching mechanism is configured to switch between a first, a second and/or a third mode, wherein in the first mode at least a portion of the light emitted from the one or more light sources propagates through one of the illumination paths; in the second mode at least a portion of the light emitted from the one or more light sources propagates through the other one of the illumination paths; and in the third mode at least a portion of the light emitted from one or more of the light sources propagates through one illumination path while simultaneously at least another portion of the light emitted from one or more of the light sources propagates through the other illumination path. At least one image detector positioned in an optical detection path, configured to detect at least a portion of fluorescent light emitted from fluorescent molecules of the illuminated sample. Another aspect concerns a method for obtaining super-resolution image data of a sample labeled with at least one type of fluorescent label comprising illuminating the sample with localization illumination light and with structured illumination light; detecting at least a portion of the fluorescent light emitted from the excited fluorescent molecules of the at least one fluorescent label, thereby obtaining at least one image of the illuminated sample; and processing the obtained at least one image of the sample image to obtain super-resolution image data.

    Abstract translation: 本发明涉及通过组合定位显微镜和结构化照明显微镜获得用至少一种类型荧光标记物标记的样品的超分辨率图像的荧光显微镜和相应方法。 在一方面，荧光显微镜包括一个或多个光源和具有结构化照明路径的照明系统，其中定位图案生成系统，用于以结构化的照明光照射样本，并且具有用于照亮样品的定位照明路径， 定位照明灯。 切换机构被配置为在第一模式，第二模式和/或第三模式之间切换，其中在第一模式中，从一个或多个光源发射的光的至少一部分传播通过照明路径之一; 在第二模式中，从一个或多个光源发射的光的至少一部分传播通过另一个照明路径; 并且在第三模式中，从一个或多个光源发射的光的至少一部分传播通过一个照明路径，同时，从一个或多个光源发射的光的至少另一部分传播通过另一个照明路径 。 位于光学检测路径中的至少一个图像检测器，被配置为检测从被照射样品的荧光分子发射的荧光的至少一部分。 另一方面涉及一种用于获得用至少一种类型的荧光标签标记的样品的超分辨率图像数据的方法，包括用定位照明光和结构化照明光照射样品; 检测从所述至少一个荧光标记的激发的荧光分子发射的荧光的至少一部分，从而获得所述被照射样品的至少一个图像; 以及处理获得的样本图像的至少一个图像以获得超分辨率图像数据。

    AN IMAGE FORMING METHOD OF A FLUORESCENT SAMPLE
    212.
    发明申请
    AN IMAGE FORMING METHOD OF A FLUORESCENT SAMPLE 审中-公开
    荧光素样品的图像形成方法

    公开(公告)号：WO2014155262A1

    公开(公告)日：2014-10-02

    申请号：PCT/IB2014/060057

    申请日：2014-03-22

    Applicant: GANDOLFI, Daniela ,  MAPELLI, Jonathan ,  D'ANGELO, Egidio ,  POZZI, Paolo

    Inventor： GANDOLFI, Daniela ,  MAPELLI, Jonathan ,  D'ANGELO, Egidio ,  POZZI, Paolo

    IPC: G02B21/00 ,  G01N21/64

    CPC classification number: G02B21/002 ,  G01N21/6458 ,  G01N2201/0675 ,  G02B21/16 ,  G02B21/365

    Abstract: A method of imaging a fluorescent sample comprising the steps of: scanning fluorescent points (9, 11) of said sample using scanner means (10, 8A, 88), thereby obtaining scanned fluorescent points; imaging said scanned fluorescent points on display means (12), said scanning comprising: predefining a scan field (2) for said sample, which comprises a set of scannable fluorescent points (9, 11); sequentially irradiating, using irradiation means (4, 8A, 8B), at least one first subset of points of said set of points and at least one second subset of said set of points, which complements said first subset with respect to said set of points. The first and second subsets can be irradiated at different focal irradiation distances (PI, P2).

    Abstract translation: 一种对荧光样品进行成像的方法，包括以下步骤：使用扫描仪装置（10,8A，88）扫描所述样品的荧光点（9,11），从而获得扫描的荧光点; 对显示装置（12）上的所述扫描荧光点进行成像，所述扫描包括：预定义所述样本的扫描场（2），其包括一组可扫描的荧光点（9,11）; 使用照射装置（4,8A，8B）顺序照射所述一组点的至少一个点的第一子集和所述一组点的至少一个第二子集，其相对于所述集合点补充所述第一子集 。 可以在不同的聚焦照射距离（PI，P2）照射第一和第二子集。

    A FAST MICROSCOPE FOR MEASURING DYNAMIC OPTICAL SIGNALS
    213.
    发明申请
    A FAST MICROSCOPE FOR MEASURING DYNAMIC OPTICAL SIGNALS 审中-公开
    用于测量动态光学信号的快速显微镜

    公开(公告)号：WO2013184918A1

    公开(公告)日：2013-12-12

    申请号：PCT/US2013/044515

    申请日：2013-06-06

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： SHTRAHMAN, Matthew ,  AHARONI, Daniel

    IPC: G02B21/00 ,  G01N21/64

    CPC classification number: G02B21/002 ,  G01N21/6408 ,  G01N21/6458 ,  G01N2201/0675 ,  G02B21/16 ,  G02B21/32 ,  G02B21/365

    Abstract: In one embodiment, a method includes instructing a pattern generator to provide an excitation pattern to a sample under test, and controlling a movable mechanism that receives an emission pattern from the sample under test responsive to the excitation pattern. The controlling includes causing the movable mechanism to sweep a representation of the emission pattern along a path, thereby creating at least one streak, and synchronizing the motion of the movable mechanism with the frame rate of an imaging device, such that a full sweep of the representation of the emission pattern along the path corresponds to approximately one frame of the imaging device. The variable intensity of each streak represents a corresponding time varying intensity of a portion of the emission pattern. The varying intensity of a portion of the emission pattern may be captured with sub-millisecond resolution.

    Abstract translation: 在一个实施例中，一种方法包括指示图案发生器向被测试样品提供激励图案，以及控制响应于激发图案从被测样品接收发射图案的可移动机构。 该控制包括使可移动机构沿着路径扫描发射图案的表示，从而产生至少一条条纹，并使可移动机构的运动与成像装置的帧速率同步，使得全扫描 沿着路径的发射图案的表示对应于成像装置的大约一帧。 每个条纹的可变强度表示发射图案的一部分的对应的时变强度。 可以以亚毫秒分辨率捕获发射图案的一部分的变化强度。

    WAVELENGTH SWEPT LIGHT SOURCE APPARATUS AND OPTICAL COHERENCE TOMOGRAPHY SYSTEM INCLUDING THE SAME
    215.
    发明申请
    WAVELENGTH SWEPT LIGHT SOURCE APPARATUS AND OPTICAL COHERENCE TOMOGRAPHY SYSTEM INCLUDING THE SAME 审中-公开
    波长光束光源装置和光学相干系统，包括它们

    公开(公告)号：WO2011099409A3

    公开(公告)日：2011-11-17

    申请号：PCT/JP2011052201

    申请日：2011-01-27

    Applicant: CANON KK ,  YOSHII MINORU ,  FUJII EIICHI

    Inventor： YOSHII MINORU ,  FUJII EIICHI

    IPC: H01S3/1055 ,  G01J3/10 ,  G01N21/17 ,  H01S3/08 ,  H01S3/081 ,  H01S3/105 ,  H01S3/106 ,  H01S5/14

    CPC classification number: H01S3/1055 ,  A61B5/0066 ,  G01J3/10 ,  G01N21/4795 ,  G01N2201/0675 ,  H01S3/08009 ,  H01S3/08013 ,  H01S3/0805 ,  H01S3/0812 ,  H01S3/105 ,  H01S3/1068 ,  H01S5/141 ,  H01S5/146

    Abstract: A swept light source apparatus in which oscillation wavelength is continuously changeable is provided. The apparatus includes, inside a resonator (103, 109), an optical amplification medium (101) that amplifies light, a first device (106) configured to disperse light emitted from the optical amplification medium and thus produce beams having different wavelengths, a second device (107) functioning as a non- focusing optical element and configured to collimate the beams having different wavelengths resulting from the dispersion by the first device, and a selecting device (108) configured to select a beam having a specific wavelength from among the beams collimated by the second device. The beam having the specific wavelength selected by the selecting device is fed back to the optical amplification medium. The first and second device may be diffraction gratings or acousto - optical devices. The selcting device may include metal-foil ribbons (301, 302) or an acousto - optical device (108).

    Abstract translation: 提供振荡波长连续变化的扫掠光源装置。 该装置包括在谐振器（103,109）内部放大光放大介质（101），第一装置（106）被配置为分散从光放大介质发射的光，从而产生具有不同波长的光束，第二 （107），其用作非聚焦光学元件并且被配置为对由所述第一装置的色散产生的具有不同波长的光束进行准直;以及选择装置（108），被配置为从所述光束中选择具有特定波长的光束 由第二个设备准直。 具有由选择装置选择的特定波长的光束被反馈到光放大介质。 第一和第二装置可以是衍射光栅或声光装置。 振动装置可以包括金属箔带（301,302）或声光装置（108）。

    METHOD AND SYSTEM FOR OPTICAL DATA ACQUISITION AND TOMOGRAPHY IMAGING OF A TURBID MEDIUM OBJECT
    216.
    发明申请
    METHOD AND SYSTEM FOR OPTICAL DATA ACQUISITION AND TOMOGRAPHY IMAGING OF A TURBID MEDIUM OBJECT 审中-公开
    涡轮介质对象的光学数据采集和图像成像的方法和系统

    公开(公告)号：WO2011035408A1

    公开(公告)日：2011-03-31

    申请号：PCT/CA2010/001445

    申请日：2010-09-22

    Applicant: LA CORPORATION DE L'ECOLE POLYTECHNIQUE DE MONTREAL B.R.C.D.T. ,  LESAGE, Frédéric ,  BÉLANGER, Samuel ,  ABRAN, Maxime ,  PROVOST, Jean

    Inventor： LESAGE, Frédéric ,  BÉLANGER, Samuel ,  ABRAN, Maxime ,  PROVOST, Jean

    IPC: G01N21/49 ,  G01N21/64 ,  G01N21/76 ,  A61B6/00

    CPC classification number: G01N21/4795 ,  A61B5/0073 ,  A61B5/0091 ,  A61B5/4312 ,  G01N21/6456 ,  G01N21/763 ,  G01N2021/1787 ,  G01N2021/479 ,  G01N2201/0675

    Abstract: A system and method for optical data acquisition of an illuminated turbid medium object, the system comprises a variable structured light detector and a controller. The light detector is adapted to retrieve light from a plurality of detection points of an output surface of the illuminated turbid medium object with a plurality of detection patterns. The controller is adapted to control the variable structured light detector for the variable structured light detector to use a detection pattern corresponding to an illumination pattern of the illuminated turbid medium object. The light detector is further adapted to optically measure a combination of retrieved light from the plurality of detection points as an optical measurement. The controller is further adapted to store an illumination pattern identifier indicative of the illumination pattern, a detection pattern identifier indicative of the detection pattern and the corresponding optical measurement.

    Abstract translation: 一种用于光学数据采集的照明混浊介质物体的系统和方法，该系统包括可变结构光检测器和控制器。 光检测器适于用多个检测图案从照明的混浊介质物体的输出表面的多个检测点检索光。 控制器适于控制可变结构光检测器的可变结构光检测器，以使用与照明混浊介质物体的照明图案相对应的检测图案。 光检测器还适于光学测量来自多个检测点的检索光的组合作为光学测量。 控制器还适于存储指示照明模式的照明模式标识符，指示检测模式的检测模式标识符和对应的光学测量。

    HIGH RESOLUTION STRUCTURED ILLUMINATION MICROSCOPY
    217.
    发明申请
    HIGH RESOLUTION STRUCTURED ILLUMINATION MICROSCOPY 审中-公开
    高分辨率结构照明显微镜

    公开(公告)号：WO2010088418A1

    公开(公告)日：2010-08-05

    申请号：PCT/US2010/022443

    申请日：2010-01-28

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  LIU, Zhaowei

    Inventor： LIU, Zhaowei

    IPC: G02B21/06 ,  G02B21/00 ,  G02B26/00 ,  G02B5/28 ,  G01N21/45 ,  G01B9/04 ,  G01B9/02

    CPC classification number: G02B21/16 ,  G01B9/04 ,  G01B11/2441 ,  G01N21/6458 ,  G01N21/648 ,  G01N2201/0635 ,  G01N2201/0675 ,  G02B5/008 ,  G02B27/60

    Abstract: Disclosed are systems, apparatus, methods and devices, including a method that includes generating two or more sequential surface plasmon interference patterns, at least one of the two or more sequential surface plasmon interference patterns being different from another of the two or more sequential surface plasmon interference patterns, and capturing respective images of a specimen resulting from the interference patterns. Also disclosed is a method that includes generating two or more sequential optical interference patterns, at least one of the two or more sequential optical interference patterns being different from another of the interference patterns, and removing from each of the generated interference patterns, using a beam stopper, a corresponding zero-order diffraction light component included in the respective generated patterns to obtain resultant corresponding two or more sequential optical interference patterns, directed at a specimen, with missing respective zero-order light components.

    Abstract translation: 公开了一种系统，装置，方法和装置，包括一种方法，包括产生两个或多个顺序表面等离子体激元干涉图案，两个或更多个顺序表面等离子体激元干涉图案中的至少一个不同于两个或多个顺序表面等离子体激元 干涉图案，并捕获由干涉图案产生的样本的各个图像。 还公开了一种方法，其包括生成两个或多个顺序光学干涉图案，所述两个或更多个顺序光学干涉图案中的至少一个与干涉图案中的另一个不同，以及使用光束从每个所生成的干涉图案中移除 阻挡器，包括在各个生成的图案中的对应的零级衍射光分量，以获得指向样本的相应的两个或更多个顺序的光学干涉图案，缺少相应的零级光分量。

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