公开(公告)号：US07754498B2

公开(公告)日：2010-07-13

申请号：US11040504

申请日：2005-01-21

Applicant: David R. Walt ,  Todd A. Dickinson

Inventor： David R. Walt ,  Todd A. Dickinson

IPC: G01N33/543 ,  G01N21/00 ,  G01N21/75

CPC classification number: C12Q1/682 ,  B01J2219/00317 ,  B01J2219/0045 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00524 ,  B01J2219/00585 ,  B01J2219/00648 ,  B01J2219/00659 ,  C12Q1/6834 ,  C40B60/14 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7703 ,  G01N33/0031 ,  G01N33/54313 ,  G01N2201/0846 ,  Y10T436/101666 ,  Y10T436/109163

Abstract: A microsphere-based analytic chemistry system is disclosed in which self-encoding microspheres having distinct characteristic optical response signatures to specific target analytes may be mixed together while the ability is retained to identify the sensor type and location of each sensor in a random dispersion of large numbers of such sensors in a sensor array using an optically interrogatable encoding scheme. An optical fiber bundle sensor is also disclosed in which individual microsphere sensors are disposed in microwells at a distal end of the fiber bundle and are optically coupled to discrete fibers or groups of fibers within the bundle. The identities of the individual sensors in the array are self-encoded by exposing the array to a reference analyte while illuminating the array with excitation light energy. A single sensor array may carry thousands of discrete sensing elements whose combined signal provides for substantial improvements in sensor detection limits, response times and signal-to-noise ratios.

Abstract translation: 公开了一种基于微球的分析化学系统，其中具有针对特定目标分析物的不同特征光学响应特征的自编码微球可以混合在一起，同时保留能力以识别大尺度随机色散中的每个传感器的传感器类型和位置 使用光学可询问编码方案的传感器阵列中的这种传感器的数量。 还公开了一种光纤束传感器，其中单个微球传感器设置在纤维束的远端处的微孔中，并且光束耦合到束内的离散纤维或纤维组。 阵列中各个传感器的身份通过将阵列暴露于参考分析物而进行自编​​码，同时用激发光能照射阵列。 单个传感器阵列可携带数千个离散感测元件，其组合信号提供传感器检测限，响应时间和信噪比方面的实质性改进。

公开(公告)号：US20100148073A1

公开(公告)日：2010-06-17

申请号：US12632187

申请日：2009-12-07

Applicant: Matthew P. Nelson ,  Patrick J. Treado

Inventor： Matthew P. Nelson ,  Patrick J. Treado

IPC: G01T1/28 ,  G01J1/58 ,  G01N21/65 ,  G01N21/64 ,  G01J3/44

CPC classification number: G01J3/2803 ,  G01J3/0221 ,  G01J3/44 ,  G01N21/474 ,  G01N21/55 ,  G01N21/645 ,  G01N21/6456 ,  G01N21/65 ,  G01N2021/6423 ,  G01N2021/6484 ,  G01N2201/0833 ,  G01N2201/0846 ,  G02B6/06

Abstract: The disclosure relates generally to methods and apparatus for using telescope optics and a fiber array spectral translator-based (“FAST”) spectroscopic system for improved imaging, spectral analysis, and interactive probing of a sample. In an embodiment, the confocality of a fiber array spectral translator-based spectroscopic system is improved through the use of structured illumination and/or structured collection of photons. User input may be received and acted upon to allow a user to interactively in real time and/or near real time view and analyze specific regions of the sample.

Abstract translation: 本公开一般涉及用于使用望远镜光学器件的方法和装置以及用于改进样品的成像，光谱分析和交互式探测的光纤阵列光谱转换器（“FAST”）光谱系统。 在一个实施例中，通过使用结构化照明和/或光子的结构化收集来提高基于光纤阵列光谱转换器的光谱系统的共焦度。 可以接收用户输入并执行操作以允许用户以实时和/或接近实时的方式进行交互式查看和分析样本的特定区域。

公开(公告)号：US20090097806A1

公开(公告)日：2009-04-16

申请号：US12249665

申请日：2008-10-10

Applicant: Bertrand Viellerobe ,  Francois Lacombe ,  Nicolas Boularot ,  Francois Doussoux ,  Nicolas Lavillonniere

Inventor： Bertrand Viellerobe ,  Francois Lacombe ,  Nicolas Boularot ,  Francois Doussoux ,  Nicolas Lavillonniere

IPC: G02B6/06

CPC classification number: G01N21/6458 ,  A61B1/00165 ,  A61B1/00186 ,  A61B1/043 ,  A61B1/0638 ,  A61B1/07 ,  A61B5/0068 ,  A61B5/0071 ,  A61B5/0084 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2201/024 ,  G01N2201/0846 ,  G01N2201/086

Abstract: An imaging device includes an illumination module comprising at least one emitter for emitting at least one excitation beam; a scanning and injection module comprising an image guide, a proximal end and a distal end of which are linked by a plurality of optical fibers; a scanning and injection optical system configured to alternately inject the at least one excitation beam into an optical fiber of the image guide from the proximal end of the image guide; a detection module comprising a detector for detecting a luminous flux collected at the distal end of the image guide, wherein at least one of the illumination module and the detection module is optically conjugated with the scanning and injection module using a conjugating optical fiber.

Abstract translation: 一种成像装置包括：照明模块，包括至少一个用于发射至少一个激发光束的发射器; 扫描和注射模块，包括图像引导件，其近端和远端由多根光纤连接; 扫描和注射光学系统，被配置为从所述图像引导件的近端交替地将所述至少一个激发光束注入到所述图像引导件的光纤中; 检测模块，包括用于检测在图像引导件的远端处收集的光通量的检测器，其中照明模块和检测模块中的至少一个使用共轭光纤与扫描和注射模块光学共轭。

公开(公告)号：US07348181B2

公开(公告)日：2008-03-25

申请号：US09287573

申请日：1999-04-06

Applicant: David R. Walt ,  Todd A. Dickinson

Inventor： David R. Walt ,  Todd A. Dickinson

IPC: G01N21/76

CPC classification number: C12Q1/682 ,  B01J2219/00317 ,  B01J2219/0045 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00524 ,  B01J2219/00585 ,  B01J2219/00648 ,  B01J2219/00659 ,  C12Q1/6834 ,  C40B60/14 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7703 ,  G01N33/0031 ,  G01N33/54313 ,  G01N2201/0846 ,  Y10T436/101666 ,  Y10T436/109163

Abstract: A microsphere-based analytic chemistry system is disclosed in which self-encoding microspheres having distinct characteristic optical response signatures to specific target analytes may be mixed together while the ability is retained to identify the sensor type and location of each sensor in a random dispersion of large numbers of such sensors in a sensor array using an optically interrogatable encoding scheme. An optical fiber bundle sensor is also disclosed in which individual microsphere sensors are disposed in microwells at a distal end of the fiber bundle and are optically coupled to discrete fibers or groups of fibers within the bundle. The identities of the individual sensors in the array are self-encoded by exposing the array to a reference analyte while illuminating the array with excitation light energy. A single sensor array may carry thousands of discrete sensing elements whose combined signal provides for substantial improvements in sensor detection limits, response times and signal-to-noise ratios.

Abstract translation: 公开了一种基于微球的分析化学系统，其中具有针对特定目标分析物的不同特征光学响应特征的自编码微球可以混合在一起，同时保留能力以识别大尺度随机色散中的每个传感器的传感器类型和位置 使用光学可询问编码方案的传感器阵列中的这种传感器的数量。 还公开了一种光纤束传感器，其中单个微球传感器设置在纤维束的远端处的微孔中，并且光束耦合到束内的离散纤维或纤维组。 阵列中各个传感器的身份通过将阵列暴露于参考分析物而进行自编​​码，同时用激发光能照射阵列。 单个传感器阵列可携带数千个离散感测元件，其组合信号提供传感器检测限，响应时间和信噪比方面的实质性改进。

公开(公告)号：US07167742B2

公开(公告)日：2007-01-23

申请号：US10142003

申请日：2002-05-09

Applicant: Nancy P. Camacho ,  Mathias P. G. Bostrom ,  Steve L. Bertha

Inventor： Nancy P. Camacho ,  Mathias P. G. Bostrom ,  Steve L. Bertha

IPC: A61B6/00

CPC classification number: G01N21/552 ,  A61B5/0059 ,  A61B5/4504 ,  G01N21/35 ,  G01N21/359 ,  G01N2021/559 ,  G01N2201/0846

Abstract: A method of evaluating the surface of a material that has a distinguishable infrared spectrum comprising (a) positioning an infrared fiber optic probe to be in contact with a surface of the sample or material at a region of interest for detecting attenuated total reflectance or within a sufficient distance from the surface of the region for detecting reflection, (b) detecting mid- or near-infrared radiation attenuated total reflectance or reflection off of the surface of the sample or the material, (c) analyzing the infrared radiation from step (b) for at least one of peak height, peak area, frequency and chemometric parameters, and (d) actuating the removal device when a signal from the infrared fiber optic probe is between pre-selected values for at least one of peak height, peak area, frequency and chemometric parameters for the sample of the material.

Abstract translation: 一种评估具有可区别的红外光谱的材料的表面的方法，包括（a）将红外光纤探针定位成与感兴趣区域的样品或材料的表面接触以检测衰减的全反射率，或者在 距离用于检测反射的区域的表面足够的距离，（b）检测中间或近红外辐射减弱了样品或材料表面的全反射或反射，（c）分析来自步骤（b）的红外辐射 ），用于峰高，峰面积，频率和化学计量参数中的至少一个，以及（d）当来自红外光纤探针的信号处于峰值高度，峰值面积之中的至少一个的预选值之间时，启动去除装置 ，样品的频率和化学计量参数。

公开(公告)号：US07142296B2

公开(公告)日：2006-11-28

申请号：US10180647

申请日：2002-06-26

Applicant: Brian T. Cunningham ,  Peter Y. Li ,  Frank DeFilippi

Inventor： Brian T. Cunningham ,  Peter Y. Li ,  Frank DeFilippi

IPC: G01J3/28

CPC classification number: G01N21/253 ,  B01L3/5085 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0829 ,  G01N21/4788 ,  G01N21/7743 ,  G01N33/54373 ,  G01N2201/0635 ,  G01N2201/08 ,  G01N2201/0833 ,  G01N2201/0846 ,  G01N2201/1248 ,  G02B5/1809 ,  G02B5/1814 ,  G02B21/06

Abstract: Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An apparatus for detecting biochemical interactions occurring on the surface of a biosensor includes a light source. A first optical fiber is coupled to the light source and illuminates the biosensor. A second optical fiber detects a wavelength reflected from the biosensor. A spectrometer determines spectra of a reflected signal from the biosensor.

公开(公告)号：US07110108B2

公开(公告)日：2006-09-19

申请号：US10480479

申请日：2002-06-12

Applicant: Gerwin Jan Puppels

Inventor： Gerwin Jan Puppels

IPC: G01J3/44

CPC classification number: G02B6/262 ,  G01N21/65 ,  G01N2021/656 ,  G01N2201/0846 ,  G02B2006/0325

Abstract: The spectrum of light, inelastically scattered by a sample (16) is measured. The light is guided through a capillary (12) from and to the sample, at least in one of these directions, through the channel no inelastic scattering of light occurs which can form an interfering background when measuring on the sample. By guiding the light through the channel, inelastic scattering of this light is prevented and it becomes possible to guide scattered light back through the channel to spectral analysis equipment (14) without problems with inelastic scattering during the guidance of the light. Preferably, the light is guided through the channel of the capillary in both directions.

Abstract translation: 测量由样品（16）非弹性散射的光谱。 通过通道，至少在这些方向中至少一个方向上，光从毛细管（12）引导到样品，不会发生光的非弹性散射，这可以在对样品进行测量时形成干扰背景。 通过引导光通过通道，防止该光的非弹性散射，并且可以在散射光的引导期间将散射光引导通过通道返回到光谱分析设备（14）而不存在非弹性散射的问题。 优选地，光在两个方向上被引导通过毛细管的通道。

公开(公告)号：US20050151971A1

公开(公告)日：2005-07-14

申请号：US10758318

申请日：2004-01-13

Applicant: Mary Tabacco ,  Marc Mittelman ,  J. Schanzie

Inventor： Mary Tabacco ,  Marc Mittelman ,  J. Schanzie

IPC: G01N17/00 ,  G01N21/64 ,  G01N21/76 ,  G01N21/84 ,  G01N21/85

CPC classification number: G01N21/8422 ,  G01N17/008 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/763 ,  G01N21/8507 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2021/6484 ,  G01N2021/8411 ,  G01N2021/8528 ,  G01N2201/0846 ,  G01N2201/1296

Abstract: Real time biofilm monitoring systems are provided. Said systems comprise single or multiple fiber-optic probes detecting wavelength-specific fluorescence from biomarkers of fouling organisms; a compact optoelectronic interface and data acquisition system interfaced with said probes, wherein said probe or probes are bifurcated and contain at least one excitation and at least one emission filter permitting the simultaneous resolution of multiple biomarkers.

Abstract translation: 提供实时生物膜监测系统。 所述系统包括检测来自污染生物体的生物标志物的波长特异性荧光的单个或多个光纤探针; 与所述探针接口的紧凑型光电接口和数据采集系统，其中所述探针或探针分叉并且包含至少一个激发和至少一个允许同时分辨多个生物标志物的发射过滤器。

公开(公告)号：US20040248161A1

公开(公告)日：2004-12-09

申请号：US10788529

申请日：2004-02-26

Inventor： Jonathan M. Rothberg ,  Joel S. Bader ,  Scott B. Dewell ,  Keith McDade ,  John W. Simpson ,  Jan Berka ,  Christopher M. Colangelo

IPC: C12Q001/68 ,  C12P019/34

CPC classification number: C12Q1/6837 ,  B01L3/502715 ,  B01L9/527 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0877 ,  B82Y30/00 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/6452 ,  G01N2201/08 ,  G01N2201/0846 ,  C12Q2531/125 ,  C12Q2535/101 ,  C12Q2565/301 ,  C12Q2565/107 ,  C12Q2525/307

Abstract: Disclosed herein are methods and apparatuses for sequencing a nucleic acid. In one aspect, the method includes annealing a population of circular nucleic acid molecules to a plurality of anchor primers linked to a solid support, and amplifying those members of the population of circular nucleic acid molecules which anneal to the target nucleic acid, and then sequencing the amplified molecules by detecting the presence of a sequence byproduct such as pyrophosphate.

Abstract translation: 本文公开了用于测序核酸的方法和装置。 一方面，该方法包括将多个环状核酸分子退火至与固体支持物连接的多个锚定引物，并扩增与靶核酸退火的环状核酸分子群体的那些成员，然后测序 通过检测序列副产物如焦磷酸盐的存在来扩增分子。

公开(公告)号：US06514277B1

公开(公告)日：2003-02-04

申请号：US09329998

申请日：1999-06-11

Applicant: Lothar Lilge ,  David Walsh

Inventor： Lothar Lilge ,  David Walsh

IPC: A61B1822

CPC classification number: G01N21/7703 ,  A61N5/0601 ,  A61N5/062 ,  G01N21/6428 ,  G01N2201/0846 ,  G01N2201/088

Abstract: A multitasking optical fiber probe for collecting dosimeter information from more than one position in a sample. The basic principle of the present invention involves using one or more different sensor zones along the length of the fiber each with a different photoactive constituent having a sufficiently unique emission spectra (spectral or temporal) to enable deconvolution of the emission spectra by the computer and therefore correlation of the detected parameter with the position of the sensor zone along the length of the optical fiber. In the broadest form of the invention the probe is embodied by only one sensor zone located at some point along the length of the fiber spaced away from the end face of the fiber. Probes are provided in which multiple sensor zones are disposed along the length of the fiber and photoactive constituents with sufficiently unique emission spectra (intensity and/or spectral shape which convey the optical information) are used in the different sensor zones so that the different spectra can be deconvoluted so that the contributions from the various etch zones can be distinguished. More than one different photoactive constituent could be incorporated into a single sensor zone for measuring several factors in the vicinity of the sensor zone. In photodynamic therapy applications the probe is isotropic in response and can be employed for all light (300 to 900nm) based medical diagnostics and therapeutics. As an extension the probe can include photosensitiser and molecular oxygen concentrations dosimetry to be used for photodynamic therapy (PDT) treatment monitoring, dosimetry and planning utilizing a mathematical model describing tissue response to PDT.

Abstract translation: 一种用于从样品中多于一个位置收集剂量计信息的多任务光纤探针。 本发明的基本原理涉及沿着纤维的长度使用一个或多个不同的传感器区域，每个具有不同的光活性组分具有足够独特的发射光谱（光谱或时间），以使计算机能够对发射光谱进行去卷积，因此 检测参数与传感器区域沿着光纤长度的位置的相关性。 在本发明的最广泛形式中，探针仅通过位于沿纤维长度的某一点处的一个传感器区域，该距离与纤维的端面间隔开。 提供探针，其中多个传感器区域沿着纤维的长度设置，并且光​​活性组分具有足够独特的发射光谱（传递光学信息的强度和/或光谱形状）被用于不同的传感器区域，使得不同的光谱可以 被去卷积，从而可以区分来自各种蚀刻区域的贡献。 可以将多于一种不同的光活性成分结合到单个传感器区域中，以测量传感器区域附近的几个因素。 在光动力学治疗应用中，探针是各向同性的响应，可用于所有基于光（300至900nm）的医疗诊断和治疗。 作为延伸，探针可以包括用于光动力治疗（PDT）治疗监测，剂量测定和计划的光敏剂和分子氧浓度剂量学，其使用描述对PDT的组织反应的数学模型。