-
公开(公告)号：US20190271630A1
公开(公告)日：2019-09-05
申请号：US16270421
申请日：2019-02-07
Applicant: Fluidigm Canada Inc.
Inventor： Alexandre Loboda
Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.
-
公开(公告)号：US20190120745A1
公开(公告)日：2019-04-25
申请号：US16165128
申请日：2018-10-19
Applicant: Fluidigm Canada Inc.
Inventor： Alexander V. Loboda , Dmitry R. Bandura , Vladimir I. Baranov
CPC classification number: G01N15/1056 , G01N2015/0065 , G01N2015/1006 , H01J49/0009 , H01J49/025 , H01J49/04 , H01J49/105 , H01J49/40
Abstract: The inventors have improved mass cytometer to facilitate its use for the analysis of particles.
-
公开(公告)号：US20190077890A1
公开(公告)日：2019-03-14
申请号：US16047927
申请日：2018-07-27
Applicant: FLUIDIGM CANADA INC.
Inventor： MITCHELL A. WINNIK , MARK NITZ , VLADIMIR BARANOV , XUDONG LOU
IPC: C08F8/32 , G01N33/58 , G01N33/532 , C08F220/36 , G01N33/574 , C08F8/14 , C08F8/42 , C08F220/56
Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).
-
公开(公告)号：US10072104B2
公开(公告)日：2018-09-11
申请号：US14875553
申请日：2015-10-05
Applicant: FLUIDIGM CANADA INC.
Inventor： Mitchell A. Winnik , Mark Nitz , Vladimir Baranov , Xudong Lou
IPC: G01N33/574 , C08F220/56 , C08F8/32 , C08F8/42 , C08F220/36 , G01N33/532 , G01N33/58 , C08F8/14
CPC classification number: C08F8/32 , C08F8/14 , C08F8/42 , C08F220/36 , C08F220/56 , G01N33/532 , G01N33/57426 , G01N33/58 , Y10T436/24
Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).
-
公开(公告)号：US09963667B2
公开(公告)日：2018-05-08
申请号：US14986431
申请日：2015-12-31
Applicant: FLUIDIGM CANADA INC.
Inventor： Alexander V. Loboda
CPC classification number: C12M25/00 , G01N1/286 , G01N33/4833 , G01N2001/045 , G01N2001/2886 , H01J49/0027 , H01J49/04
Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.
-
Patent Agency Ranking
公开(公告)号：US09952132B2
公开(公告)日：2018-04-24
申请号：US15408806
申请日：2017-01-18
Applicant: Fluidigm Canada Inc.
Inventor： Alexandre Loboda
CPC classification number: G01N15/10 , G01N2015/0065 , G01N2015/1006 , H01J49/0463 , H01J49/105 , H01J49/40
Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.
-
公开(公告)号：US20170098532A1
公开(公告)日：2017-04-06
申请号：US15231592
申请日：2016-08-08
Applicant: Fluidigm Canada Inc.
Inventor： RAYMOND JONG
CPC classification number: H01J49/0445 , G01N15/06 , G01N15/10 , G01N35/10 , G01N2015/0065 , G01N2015/1006 , H01J49/045 , H01J49/105
Abstract: In a mass cytometer or mass spectrometer, a sample of elemental tagged particles is transferred from a dispersion to a gas flow through a carrier aerosol spray for atomization and ionization by inductively coupled plasma (ICP) source. The configuration of the sample transfer apparatus allow for total consumption of the sample by passing the sample spray through a deceleration stage to decelerate the spray of particles from its high velocity expansion. Following the deceleration stage, the decelerated sample of particles can be accelerated and focused through an acceleration stage for transferring into the ICP. This effectively improves the particle transfer between the sample spray and the ICP.
-
公开(公告)号：US20160260598A1
公开(公告)日：2016-09-08
申请号：US15016134
申请日：2016-02-04
Applicant: FLUIDIGM CANADA INC.
Inventor： Alexander V. Loboda , Vladimir I. Baranov
IPC: H01J49/10 , H01J49/04 , G01N33/483 , G01N15/10
CPC classification number: H01J49/105 , G01N15/10 , G01N33/4833 , G01N2015/0065 , G01N2015/1006 , H01J49/0459 , H01J49/0463
Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.
Abstract translation: 本发明涉及使用激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）分析样品的方法和装置。 本发明提供方法和装置，其中个体消融羽流被明显地捕获并转移到ICP，随后通过质谱法分析。
-
公开(公告)号：US09431227B2
公开(公告)日：2016-08-30
申请号：US14594319
申请日：2015-01-12
Applicant: FLUIDIGM CANADA INC.
Inventor： Raymond Jong
CPC classification number: H01J49/0445 , G01N15/06 , G01N15/10 , G01N35/10 , G01N2015/0065 , G01N2015/1006 , H01J49/045 , H01J49/105
Abstract: In a mass cytometer or mass spectrometer, a sample of elemental tagged particles is transferred from a dispersion to a gas flow through a carrier aerosol spray for atomization and ionization by inductively coupled plasma (ICP) source. The configuration of the sample transfer apparatus allow for total consumption of the sample by passing the sample spray through a deceleration stage to decelerate the spray of particles from its high velocity expansion. Following the deceleration stage, the decelerated sample of particles can be accelerated and focused through an acceleration stage for transferring into the ICP. This effectively improves the particle transfer between the sample spray and the ICP.
-
公开(公告)号：US20160194590A1
公开(公告)日：2016-07-07
申请号：US14986431
申请日：2015-12-31
Applicant: FLUIDIGM CANADA INC.
Inventor： Alexander V. Loboda
IPC: C12M1/12 , G01N33/483 , H01J49/00 , G01N1/28
CPC classification number: C12M25/00 , G01N1/286 , G01N33/4833 , G01N2001/045 , G01N2001/2886 , H01J49/0027 , H01J49/04
Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.
Abstract translation: 公开了将生物样品递送到质谱仪的ICP源的装置和方法。 生物材料设置在载体上的多个离散位置上。 多个离散部位配置成保留生物材料并在施加能量时释放生物材料。 载体位于气体导管附近，并且在从离散位置释放时，生物材料被夹带在气流中，其将生物材料的离散部分通过导管输送到ICP源以通过质量细胞计量分析。 该装置和方法可以向质谱仪或质谱仪提供生物材料的分立部分的连续流。
-
-
-
-
-
-
-
-
-
Search Results
210
records
(took 0.036 seconds)
