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公开(公告)号：US11264221B2
公开(公告)日：2022-03-01
申请号：US17253538
申请日：2019-06-18
Applicant: Fluidigm Canada Inc. , Paul Corkum
Inventor： Paul Corkum , Alexander V. Loboda
IPC: H01J49/04 , G01N33/68 , G02B21/33 , H01J37/20 , H01J37/28 , H01J49/00 , H01J49/02 , H01J49/06 , H01J49/10 , H01J49/40 , H01J49/16 , H01J49/14
Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMCTM) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.
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公开(公告)号：US20180312794A1
公开(公告)日：2018-11-01
申请号：US15966432
申请日：2018-04-30
Applicant: Fluidigm Canada Inc.
Inventor： Alexander V. Loboda
CPC classification number: C12M25/00 , G01N1/286 , G01N33/4833 , G01N2001/045 , G01N2001/2886 , H01J49/0027 , H01J49/04
Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.
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公开(公告)号：US20160195466A1
公开(公告)日：2016-07-07
申请号：US14983463
申请日：2015-12-29
Applicant: Fluidigm Canada Inc.
Inventor： Alexander V. Loboda , Dmitry R. Bandura , Vladimir I. Baranov
CPC classification number: G01N15/1056 , G01N2015/0065 , G01N2015/1006 , H01J49/0009 , H01J49/025 , H01J49/04 , H01J49/105 , H01J49/40
Abstract: The inventors have improved mass cytometer to facilitate its use for the analysis of particles.
Abstract translation: 发明人已经改进了质谱仪以便于其用于分析颗粒。
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公开(公告)号：US20160139141A1
公开(公告)日：2016-05-19
申请号：US14940951
申请日：2015-11-13
Applicant: Fluidigm Canada Inc.
Inventor： Alexander V. Loboda
IPC: G01N33/68
CPC classification number: G01N33/6848 , G01N33/58 , G01N2458/15
Abstract: Methods of imaging a biological sample by mass cytometry using molecular tagging are disclosed.
Abstract translation: 公开了通过使用分子标记的质谱法对生物样品进行成像的方法。
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公开(公告)号：US20210333173A1
公开(公告)日：2021-10-28
申请号：US17274331
申请日：2019-09-10
Applicant: Fluidigm Canada Inc.
Inventor： DAAF SANDKUIJL , Alexander V. Loboda , ADAM CAREW , MINALINI LAKSHMAN
Abstract: This disclosure relates to systems and methods for high speed modulation sample imaging. Disclosed herein are systems and methods for performing imaging mass cytometry, including analysis of labelling atoms by elemental (e.g., atomic) mass spectrometry. Aspects include a sampling system having, and method of using, a femtosecond (fs) laser and/or laser scanning. Alternatively or in addition, aspects include systems and methods for co-registering other imaging modalities with imaging mass cytometry.
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Patent Agency Ranking
公开(公告)号：US20190120745A1
公开(公告)日：2019-04-25
申请号：US16165128
申请日：2018-10-19
Applicant: Fluidigm Canada Inc.
Inventor： Alexander V. Loboda , Dmitry R. Bandura , Vladimir I. Baranov
CPC classification number: G01N15/1056 , G01N2015/0065 , G01N2015/1006 , H01J49/0009 , H01J49/025 , H01J49/04 , H01J49/105 , H01J49/40
Abstract: The inventors have improved mass cytometer to facilitate its use for the analysis of particles.
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公开(公告)号：US09963667B2
公开(公告)日：2018-05-08
申请号：US14986431
申请日：2015-12-31
Applicant: FLUIDIGM CANADA INC.
Inventor： Alexander V. Loboda
CPC classification number: C12M25/00 , G01N1/286 , G01N33/4833 , G01N2001/045 , G01N2001/2886 , H01J49/0027 , H01J49/04
Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.
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公开(公告)号：US20160260598A1
公开(公告)日：2016-09-08
申请号：US15016134
申请日：2016-02-04
Applicant: FLUIDIGM CANADA INC.
Inventor： Alexander V. Loboda , Vladimir I. Baranov
IPC: H01J49/10 , H01J49/04 , G01N33/483 , G01N15/10
CPC classification number: H01J49/105 , G01N15/10 , G01N33/4833 , G01N2015/0065 , G01N2015/1006 , H01J49/0459 , H01J49/0463
Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.
Abstract translation: 本发明涉及使用激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）分析样品的方法和装置。 本发明提供方法和装置，其中个体消融羽流被明显地捕获并转移到ICP，随后通过质谱法分析。
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公开(公告)号：US20160194590A1
公开(公告)日：2016-07-07
申请号：US14986431
申请日：2015-12-31
Applicant: FLUIDIGM CANADA INC.
Inventor： Alexander V. Loboda
IPC: C12M1/12 , G01N33/483 , H01J49/00 , G01N1/28
CPC classification number: C12M25/00 , G01N1/286 , G01N33/4833 , G01N2001/045 , G01N2001/2886 , H01J49/0027 , H01J49/04
Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.
Abstract translation: 公开了将生物样品递送到质谱仪的ICP源的装置和方法。 生物材料设置在载体上的多个离散位置上。 多个离散部位配置成保留生物材料并在施加能量时释放生物材料。 载体位于气体导管附近，并且在从离散位置释放时，生物材料被夹带在气流中，其将生物材料的离散部分通过导管输送到ICP源以通过质量细胞计量分析。 该装置和方法可以向质谱仪或质谱仪提供生物材料的分立部分的连续流。
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公开(公告)号：US12000838B2
公开(公告)日：2024-06-04
申请号：US17050716
申请日：2019-04-26
Applicant: Fluidigm Canada Inc.
Inventor： Alexander V. Loboda , Olga Ornatsky , Vladimir Baranov , Nikita Zabinyakov , Anastasia Mavropoulos , Daniel Majonis , Bedilu Allo , Taunia Closson
CPC classification number: G01N33/6848 , C07K1/13 , G01N1/28 , G01N2458/15 , G01N2560/00
Abstract: Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. The embodiments comprising methods for quantifying one or more analytes within a sample, comprising the steps of: (a) providing the sample, wherein the one or more analytes are immobilized to a sample carrier, wherein the sample has been labelled with one or more mass tags comprising one or more labelling atoms, (b) performing mass cytometry on the sample to determine the level of the one or more labelling atoms, wherein the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes.
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