公开(公告)号：WO1997046319A1

公开(公告)日：1997-12-11

申请号：PCT/US1997009649

申请日：1997-06-03

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  ROACH, David, J. ,  JOHNSTON, Richard, F.

IPC: B01L03/02

CPC classification number: B01L3/0262 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01L3/0248 ,  B01L3/0255 ,  B01L2300/0838 ,  C40B60/14 ,  G01N2035/1037 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A microspot deposition system (12) featuring a hollow cylindrical wall (26) extending from a closed end (28), terminating in an open end (30) and including a longitudinal gap (34) extending from the open end (30) toward the closed end (28) to allow the rapid exhaustion of the atmosphere and efficient cleaning within the cylindrical wall (26). The cylindrical wall (26) defines a lumen (32) with both the lumen (32) and the gap (34) adapted to facilitate capillary action of liquid in fluid communication therewith to form a meniscus (46) proximate to the open end (30). To facilitate deposition of liquid contained within the lumen (32), the gap (34) may be tapered so that it is narrowest proximate to the open end (30). The narrowed portion of the gap (34) results in a meniscus (46) having a reduced area to ensure preferential fluid flow toward the open end (30), which facilitates deposition via capillary action between the liquid in the lumen (32) and a working surface (52).

Abstract translation: 一种微孔沉积系统（12），其具有从封闭端（28）延伸的中空圆柱形壁（26），终止于开口端（30），并且包括从开口端（30）延伸的纵向间隙（34） 闭合端（28）以允许气氛的快速耗尽并且在圆柱形壁（26）内进行有效的清洁。 圆柱形壁（26）限定内腔（32），内腔（32）和间隙（34）都适于促进与其流体连通的液体的毛细作用，以形成靠近开口端（30）的弯月面（46） ）。 为了便于包含在内腔（32）内的液体的沉积，间隙（34）可以是锥形的，使得其在开口端（30）附近最窄。 间隙（34）的变窄的部分导致弯液面（46）具有减小的面积，以确保朝向开口端（30）的优先流体流动，这有利于通过管腔（32）中的液体和 工作面（52）。

公开(公告)号：WO1997003350A1

公开(公告)日：1997-01-30

申请号：PCT/US1996010311

申请日：1996-06-14

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  BASHKIN, John, S. ,  BARKER, David, L. ,  JOHNSTON, Richard, F.

IPC: G01N27/26

CPC classification number: G01N27/44747

Abstract: A capillary electrophoresis separation matrix for single-stranded nucleic acids, along with methods for using and preparing the matrix, are disclosed. The separation matrix provides denaturing conditions and contains hydroxyethyl cellulose (HEC) in combination with urea, and preferably also includes formamide. The separation matrix may be used for DNA sizing and sequencing applications and provides a single-base resolution to approximately 500 base pairs. In the figure, line (10) represents peak spacing and line (20) representa peak width of the HEC/urea/formamide separation matrix. Line (15) and line (25) represent the peak spacing and peak width, respectively, of the prior art matrix. The separation matrix is inexpensive, easy to prepare, requires no polymerization steps, and is of low enough viscosity to be pumped easily into and out of capillary tubes for electrophoresis. The low viscosity allows for high throughput of samples and reuse of the capillary tubes for numerous separations.

Abstract translation: 公开了用于单链核酸的毛细管电泳分离基质，以及用于使用和制备基质的方法。 分离基质提供变性条件并含有与尿素组合的羟乙基纤维素（HEC），并且优选还包括甲酰胺。 分离矩阵可用于DNA大小和测序应用，并提供大约500个碱基对的单碱基分辨率。 在图中，线（10）表示峰间距，线（20）表示HEC /尿素/甲酰胺分离基质的峰宽。 线（15）和线（25）分别代表现有技术矩阵的峰间距和峰宽。 分离基质便宜，易于制备，不需要聚合步骤，并且具有足够低的粘度以便容易地泵入和流出用于电泳的毛细管。 低粘度允许样品的高通量和用于多次分离的毛细管的再利用。

公开(公告)号：WO1996036062A1

公开(公告)日：1996-11-14

申请号：PCT/US1996005120

申请日：1996-04-09

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  KAIN, Robert, C. ,  MILLER, Michael, F. ,  MAJLOF, Lars

IPC: H01J03/14

CPC classification number: G01N21/6452

Abstract: An LED point imaging scanner for stimulating and reading fluorescent and reflective signal radiation from a target sample (10) is disclosed. LED light source (12) is mounted on scan head (20) and focused into pinhole aperture (15) of spatial filter (30), then collimated and focused to a spot (17) on sample (10). The spatial filter, or an optical fiber equivalent, effectively restricts the incoherent LED light and creates a point light source. Signal radiation from sample (10) is collected through scan head (20) and the returning light beam is collimated and focused onto detection means (40). A spatial filter (22) in the path of the returning beam may also be utilized. Detection means (40) is either a small detector directly mounted onto the scan head, or an end of an optical fiber which transmits the point image to a remotely-positioned stationary detector. The scan head is moved along one or two axes relative to the sample.

Abstract translation: 公开了一种用于刺激和读取来自目标样品（10）的荧光和反射信号辐射的LED点成像扫描仪。 LED光源（12）安装在扫描头（20）上并聚焦到空间滤光器（30）的针孔（15）中，然后准直并聚焦到样品（10）上的点（17）上。 空间滤波器或光纤等效物质有效地限制了非相干LED光并产生点光源。 来自样品（10）的信号辐射通过扫描头（20）收集，并且返回的光束被准直并聚焦到检测装置（40）上。 还可以利用返回光束的路径中的空间滤光器（22）。 检测装置（40）是直接安装在扫描头上的小检测器或将点图像传输到远程定位的固定检测器的光纤的端部。 扫描头相对于样品沿着一个或两个轴线移动。

公开(公告)号：WO2018146691A1

公开(公告)日：2018-08-16

申请号：PCT/IL2018/050165

申请日：2018-02-13

Applicant: MOLECULAR DYNAMICS LIMITED

Inventor： KENIG, Tal ,  DEVIR, Zvi

IPC: G01T1/164 ,  G01T1/24 ,  G01T1/161

CPC classification number: G01T1/249 ,  G01T1/161

Abstract: A method of processing a SPECT image of a region of interest is disclosed. The SPECT image was obtained using at least one gamma detector detecting gamma radiation from the region of interest at multiple detector configurations, and the method includes: obtaining data indicative of the detector configurations and their spatial relationships to the region of interest; determining a resolution level for each of a plurality of directions in each point in the image based on the data obtained; and processing the image based on the resolution levels determined.

公开(公告)号：WO2018146673A1

公开(公告)日：2018-08-16

申请号：PCT/IL2018/050129

申请日：2018-02-06

Applicant: MOLECULAR DYNAMICS LIMITED ,  KENIG, Tal ,  DEVIR, Zvi

Inventor： KENIG, Tal ,  DEVIR, Zvi

IPC: G01T1/164

Abstract: The disclosure describes methods and apparatuses for scanning a region of interest (ROI) by a gamma detector. An exemplary method includes determining, for each of multiple detector configurations, a respective weight based on an absorption profile, associating each of a plurality of portions of the ROI with a respective gamma attenuation value; and detecting gamma radiation from multiple detector configurations for time periods allocated among the detector configurations based on the weights determined.

公开(公告)号：WO0168898A2

公开(公告)日：2001-09-20

申请号：PCT/US0108130

申请日：2001-03-14

Applicant: MOLECULAR DYNAMICS INC ,  LIU SHAORONG

Inventor： LIU SHAORONG

IPC: G01N27/447 ,  G01N37/00 ,  C12Q

CPC classification number: G01N27/44791 ,  G01N27/44782

Abstract: A microfabricated capillary array electrophoresis chip includes a planar substrate having a first major surface defining converging first and second elongate separation channels. Each separation channel extends between an associated cathode port and anode port defined by the first major surface. The substrate further comprises a first perimetrical edge segment extending substantially along the first separation channel, and a second perimetrical edge segment extending substantially along the second separation channel. The perimetrical edge segments of a pair of chips of the present invention are cooperatively engageable so as to provide an electrophoresis separation plate having pseudoradially-aligned separation channels.

Abstract translation: 微细毛细管阵列电泳芯片包括具有限定会聚的第一和第二细长分离通道的第一主表面的平面基底。 每个分离通道在由第一主表面限定的相关联的阴极端口和阳极端口之间延伸。 衬底还包括基本沿着第一分离通道延伸的第一周边边缘段和基本上沿着第二分隔通道延伸的第二周边边缘段。 本发明的一对芯片的周边边缘段可协作地接合，以便提供具有伪随机对准分离通道的电泳分离板。

公开(公告)号：WO0109389A3

公开(公告)日：2001-08-16

申请号：PCT/US0021182

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS INC

Inventor： HADD ANDY ,  JOVANOVICH STEVAN

IPC: C12N15/09 ,  B01D57/02 ,  B01J4/00 ,  B01J19/00 ,  B01J19/26 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/38 ,  C12Q1/68 ,  C40B40/02 ,  C40B40/06 ,  C40B50/06 ,  C40B60/04 ,  C40B60/14 ,  G01N1/00 ,  G01N27/447 ,  G01N31/20 ,  G01N33/50 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  G01N37/00

CPC classification number: C12Q1/6869 ,  B01J2219/00286 ,  B01J2219/00369 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00691 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/5025 ,  B01L7/52 ,  B01L2300/0838 ,  B01L2300/1844 ,  B01L2400/0409 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/6834 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/809 ,  Y10T436/113332 ,  Y10T436/143333 ,  Y10T436/2575 ,  C12Q2565/518 ,  C12Q2535/101 ,  C12Q2527/125

Abstract: Methods for preparing nanoscale reactions using nucleic acids are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Alternatively, the saturably bound nucleic acid is eluted, dispensing a metered amount of nucleic acid for subsequent reaction in a separate chamber. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput nucleic acid sequencing reactions are also provided.

Abstract translation: 介绍了使用核酸制备纳米级反应的方法。 在反应室的内表面上，通常是毛细管，可饱和地但可逆地捕获核酸。 除去过量的核酸并直接在毛细管内进行反应。 或者，可饱和结合的核酸被洗脱，分配计量的量的核酸用于在单独的室中进行后续反应。 还提供了用于实现本发明的方法的装置和有利地设计以利用用于高通量核酸测序反应的方法的系统。

公开(公告)号：WO0157270A2

公开(公告)日：2001-08-09

申请号：PCT/US0100661

申请日：2001-01-30

Applicant: MOLECULAR DYNAMICS INC ,  PENN SHARRON G ,  HANZEL DAVID K ,  CHEN WENSHENG ,  RANK DAVID R

Inventor： PENN SHARRON G ,  HANZEL DAVID K ,  CHEN WENSHENG ,  RANK DAVID R

IPC: A61K38/00 ,  C07K14/47 ,  C07K14/705 ,  C12N15/10 ,  C12N15/66 ,  C12Q1/68 ,  G06F19/18 ,  G06F19/20 ,  G06F19/22 ,  G06F19/26 ,  G06F19/00

CPC classification number: C12N15/1089 ,  A01K2217/05 ,  A01K2217/075 ,  A61K38/00 ,  C07K14/47 ,  C07K14/4748 ,  C07K14/705 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/40 ,  C07K2319/60 ,  C12N15/66 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158 ,  G06F19/18 ,  G06F19/20 ,  G06F19/22 ,  G06F19/26 ,  C12Q2565/501 ,  C12Q2539/105

Abstract: A single exon nucleic acid microarray comprising a plurality of single exon nucleic acid probes for measuring gene expression in a sample derived from human HBL 100 cells is described. Also described are single exon nucleic acid probes expressed in the HBL 100 cells and their use in methods for detecting gene expression.

Abstract translation: 描述了包含用于测量衍生自人HBL 100细胞的样品中的基因表达的多个单个外显子核酸探针的单个外显子核酸微阵列。 还描述了在HBL 100细胞中表达的单个外显子核酸探针及其在检测基因表达的方法中的用途。

公开(公告)号：WO1998012536A1

公开(公告)日：1998-03-26

申请号：PCT/US1997016224

申请日：1997-09-12

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  KAIN, Robert, C.

IPC: G01N21/00

CPC classification number: G02B21/02

Abstract: The present invention features two flat-field, telecentric, infinite conjugate, achromatic objectives (32 and 34) each of which has an external pupil lying in a common plane located equidistant from the two objectives, defining a mechanically accessible central pupil (30) of an imaging system centered in the common plane (28). Each of the objectives is afocal in the common plane, with one of the lenses forming a focal plane proximate to a sample. The lenses are adapted to provide varying levels of magnification while keeping constant the number of resolvable points in the field of view. An array detector (14) is positioned proximate to a focal plane formed of the remaining objective lens (34). The double objective lens assembly is described as being included in transillumination and epi-illumination systems.

Abstract translation: 本发明具有两个平场，远心，无限共轭，消色差物体（32和34），每个物体具有位于与两个物镜等距离的公共平面中的外部光瞳，限定了机械可接近的中心瞳孔（30） 以公共平面（28）为中心的成像系统。 每个目标在公共平面中是无焦点的，其中一个透镜形成靠近样本的焦平面。 透镜适于提供不同水平的放大倍率，同时保持视场中可分辨点的数量不变。 阵列检测器（14）被定位成靠近由剩余的物镜（34）形成的焦平面。 双重物镜组件被描述为包括在透照和外照明系统中。

公开(公告)号：WO1996021855A1

公开(公告)日：1996-07-18

申请号：PCT/US1996000348

申请日：1996-01-11

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  NORRIS, Michael, C.

IPC: G01N21/01

CPC classification number: B01L3/5085 ,  B01L9/523 ,  B01L2200/025 ,  B01L2300/0829 ,  G01N21/13 ,  G01N35/028 ,  Y10S436/809

Abstract: A self-aligning mechanism for positioning analyte receptacles (108) comprises a loading carriage (200) which bears a tray (244). In one embodiment, the carriage (200) is slidingly mounted in a structure (202) and includes three guide pins (222, 224, 226) and a stop pin (238). In this embodiment, the tray (244) has a leaf spring (280) attached at the rear thereof, a lateral protuberance for contacting the structure (202), and two frontal and one lateral oblique slots (252, 254, 258). A pair of vertical contact planes (272, 274), orthogonally disposed with respect to each other, are formed on the top surface of the tray (244).

Abstract translation: 用于定位分析物容器（108）的自对准机构包括承载托盘（244）的装载托架（200）。 在一个实施例中，托架（200）滑动地安装在结构（202）中，并且包括三个引导销（222,224,226）和止动销（238）。 在该实施例中，托盘（244）具有附接在其后部的板簧（280），用于接触结构（202）的横向突起以及两个正面和一个横向倾斜槽（252,254,258）。 在托盘（244）的顶表面上形成有一对垂直接触平面（272,274），它们相对于彼此正交设置。