公开(公告)号：WO1996018205A1

公开(公告)日：1996-06-13

申请号：PCT/US1995013531

申请日：1995-10-20

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  KAIN, Robert, C.

IPC: H01J03/14

CPC classification number: G02B21/002 ,  G02B21/16

Abstract: Fluorescence imaging system includes objective (21) which is achromatic and has an external entrance pupil (29), serves as a condenser for the system, and positioned above sample (23) in close proximity to one another. Laser (18) directs collimated light (19) to scan device (20) located at entrance pupil (29). Scan device (20) reflects, refracts, or diffracts light through objective (21) to illuminate a spot (22) on the sample's surface, and illuminates a line or an area on the sample surface by varying the angle of laser light into objective (21). Sample (23) emits fluorescent light (24) in response to the illumination. The fluorescent light (24) is collected by objective (21) and passes through the system along the path of the illumination light. Wavelength-discriminating dichroic filter (25), placed along the optical axis between laser (18) and objective (21), directs fluorescent light (24) onto photodetector (26) to produce a signal representing the sample surface emitting the fluorescent light. Display (37) displays the digitized data in a raster format.

Abstract translation: 荧光成像系统包括不消色的物镜（21），并具有外部入射光瞳（29），用作系统的冷凝器，并且位于彼此靠近的样品（23）上方。 激光器（18）将准直光（19）引导到位于入射光瞳（29）处的扫描装置（20）。 扫描装置（20）通过物镜（21）反射，折射或衍射光以照射样品表面上的斑点（22），并且通过将激光的角度改变为目标来照射样品表面上的线或区域 21）。 样品（23）响应于照明而发射荧光（24）。 荧光灯（24）由物镜（21）收集并沿着照明光的路径通过系统。 沿着激光（18）和物镜（21）之间的光轴放置的波长鉴别二向色滤光器（25）将荧光（24）引导到光电检测器（26）上，以产生表示发射荧光的样品表面的信号。 显示（37）以光栅格式显示数字化数据。

公开(公告)号：WO1998012536A1

公开(公告)日：1998-03-26

申请号：PCT/US1997016224

申请日：1997-09-12

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  KAIN, Robert, C.

IPC: G01N21/00

CPC classification number: G02B21/02

Abstract: The present invention features two flat-field, telecentric, infinite conjugate, achromatic objectives (32 and 34) each of which has an external pupil lying in a common plane located equidistant from the two objectives, defining a mechanically accessible central pupil (30) of an imaging system centered in the common plane (28). Each of the objectives is afocal in the common plane, with one of the lenses forming a focal plane proximate to a sample. The lenses are adapted to provide varying levels of magnification while keeping constant the number of resolvable points in the field of view. An array detector (14) is positioned proximate to a focal plane formed of the remaining objective lens (34). The double objective lens assembly is described as being included in transillumination and epi-illumination systems.

Abstract translation: 本发明具有两个平场，远心，无限共轭，消色差物体（32和34），每个物体具有位于与两个物镜等距离的公共平面中的外部光瞳，限定了机械可接近的中心瞳孔（30） 以公共平面（28）为中心的成像系统。 每个目标在公共平面中是无焦点的，其中一个透镜形成靠近样本的焦平面。 透镜适于提供不同水平的放大倍率，同时保持视场中可分辨点的数量不变。 阵列检测器（14）被定位成靠近由剩余的物镜（34）形成的焦平面。 双重物镜组件被描述为包括在透照和外照明系统中。

公开(公告)号：WO1996021855A1

公开(公告)日：1996-07-18

申请号：PCT/US1996000348

申请日：1996-01-11

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  NORRIS, Michael, C.

IPC: G01N21/01

CPC classification number: B01L3/5085 ,  B01L9/523 ,  B01L2200/025 ,  B01L2300/0829 ,  G01N21/13 ,  G01N35/028 ,  Y10S436/809

Abstract: A self-aligning mechanism for positioning analyte receptacles (108) comprises a loading carriage (200) which bears a tray (244). In one embodiment, the carriage (200) is slidingly mounted in a structure (202) and includes three guide pins (222, 224, 226) and a stop pin (238). In this embodiment, the tray (244) has a leaf spring (280) attached at the rear thereof, a lateral protuberance for contacting the structure (202), and two frontal and one lateral oblique slots (252, 254, 258). A pair of vertical contact planes (272, 274), orthogonally disposed with respect to each other, are formed on the top surface of the tray (244).

Abstract translation: 用于定位分析物容器（108）的自对准机构包括承载托盘（244）的装载托架（200）。 在一个实施例中，托架（200）滑动地安装在结构（202）中，并且包括三个引导销（222,224,226）和止动销（238）。 在该实施例中，托盘（244）具有附接在其后部的板簧（280），用于接触结构（202）的横向突起以及两个正面和一个横向倾斜槽（252,254,258）。 在托盘（244）的顶表面上形成有一对垂直接触平面（272,274），它们相对于彼此正交设置。

公开(公告)号：WO1997046319A1

公开(公告)日：1997-12-11

申请号：PCT/US1997009649

申请日：1997-06-03

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  ROACH, David, J. ,  JOHNSTON, Richard, F.

IPC: B01L03/02

CPC classification number: B01L3/0262 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01L3/0248 ,  B01L3/0255 ,  B01L2300/0838 ,  C40B60/14 ,  G01N2035/1037 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A microspot deposition system (12) featuring a hollow cylindrical wall (26) extending from a closed end (28), terminating in an open end (30) and including a longitudinal gap (34) extending from the open end (30) toward the closed end (28) to allow the rapid exhaustion of the atmosphere and efficient cleaning within the cylindrical wall (26). The cylindrical wall (26) defines a lumen (32) with both the lumen (32) and the gap (34) adapted to facilitate capillary action of liquid in fluid communication therewith to form a meniscus (46) proximate to the open end (30). To facilitate deposition of liquid contained within the lumen (32), the gap (34) may be tapered so that it is narrowest proximate to the open end (30). The narrowed portion of the gap (34) results in a meniscus (46) having a reduced area to ensure preferential fluid flow toward the open end (30), which facilitates deposition via capillary action between the liquid in the lumen (32) and a working surface (52).

Abstract translation: 一种微孔沉积系统（12），其具有从封闭端（28）延伸的中空圆柱形壁（26），终止于开口端（30），并且包括从开口端（30）延伸的纵向间隙（34） 闭合端（28）以允许气氛的快速耗尽并且在圆柱形壁（26）内进行有效的清洁。 圆柱形壁（26）限定内腔（32），内腔（32）和间隙（34）都适于促进与其流体连通的液体的毛细作用，以形成靠近开口端（30）的弯月面（46） ）。 为了便于包含在内腔（32）内的液体的沉积，间隙（34）可以是锥形的，使得其在开口端（30）附近最窄。 间隙（34）的变窄的部分导致弯液面（46）具有减小的面积，以确保朝向开口端（30）的优先流体流动，这有利于通过管腔（32）中的液体和 工作面（52）。

公开(公告)号：WO1997003350A1

公开(公告)日：1997-01-30

申请号：PCT/US1996010311

申请日：1996-06-14

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  BASHKIN, John, S. ,  BARKER, David, L. ,  JOHNSTON, Richard, F.

IPC: G01N27/26

CPC classification number: G01N27/44747

Abstract: A capillary electrophoresis separation matrix for single-stranded nucleic acids, along with methods for using and preparing the matrix, are disclosed. The separation matrix provides denaturing conditions and contains hydroxyethyl cellulose (HEC) in combination with urea, and preferably also includes formamide. The separation matrix may be used for DNA sizing and sequencing applications and provides a single-base resolution to approximately 500 base pairs. In the figure, line (10) represents peak spacing and line (20) representa peak width of the HEC/urea/formamide separation matrix. Line (15) and line (25) represent the peak spacing and peak width, respectively, of the prior art matrix. The separation matrix is inexpensive, easy to prepare, requires no polymerization steps, and is of low enough viscosity to be pumped easily into and out of capillary tubes for electrophoresis. The low viscosity allows for high throughput of samples and reuse of the capillary tubes for numerous separations.

Abstract translation: 公开了用于单链核酸的毛细管电泳分离基质，以及用于使用和制备基质的方法。 分离基质提供变性条件并含有与尿素组合的羟乙基纤维素（HEC），并且优选还包括甲酰胺。 分离矩阵可用于DNA大小和测序应用，并提供大约500个碱基对的单碱基分辨率。 在图中，线（10）表示峰间距，线（20）表示HEC /尿素/甲酰胺分离基质的峰宽。 线（15）和线（25）分别代表现有技术矩阵的峰间距和峰宽。 分离基质便宜，易于制备，不需要聚合步骤，并且具有足够低的粘度以便容易地泵入和流出用于电泳的毛细管。 低粘度允许样品的高通量和用于多次分离的毛细管的再利用。

公开(公告)号：WO1996036062A1

公开(公告)日：1996-11-14

申请号：PCT/US1996005120

申请日：1996-04-09

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  KAIN, Robert, C. ,  MILLER, Michael, F. ,  MAJLOF, Lars

IPC: H01J03/14

CPC classification number: G01N21/6452

Abstract: An LED point imaging scanner for stimulating and reading fluorescent and reflective signal radiation from a target sample (10) is disclosed. LED light source (12) is mounted on scan head (20) and focused into pinhole aperture (15) of spatial filter (30), then collimated and focused to a spot (17) on sample (10). The spatial filter, or an optical fiber equivalent, effectively restricts the incoherent LED light and creates a point light source. Signal radiation from sample (10) is collected through scan head (20) and the returning light beam is collimated and focused onto detection means (40). A spatial filter (22) in the path of the returning beam may also be utilized. Detection means (40) is either a small detector directly mounted onto the scan head, or an end of an optical fiber which transmits the point image to a remotely-positioned stationary detector. The scan head is moved along one or two axes relative to the sample.

Abstract translation: 公开了一种用于刺激和读取来自目标样品（10）的荧光和反射信号辐射的LED点成像扫描仪。 LED光源（12）安装在扫描头（20）上并聚焦到空间滤光器（30）的针孔（15）中，然后准直并聚焦到样品（10）上的点（17）上。 空间滤波器或光纤等效物质有效地限制了非相干LED光并产生点光源。 来自样品（10）的信号辐射通过扫描头（20）收集，并且返回的光束被准直并聚焦到检测装置（40）上。 还可以利用返回光束的路径中的空间滤光器（22）。 检测装置（40）是直接安装在扫描头上的小检测器或将点图像传输到远程定位的固定检测器的光纤的端部。 扫描头相对于样品沿着一个或两个轴线移动。

公开(公告)号：WO1997028439A1

公开(公告)日：1997-08-07

申请号：PCT/US1997001461

申请日：1997-01-31

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  KAIN, Robert, C. ,  ALEXAY, Christopher, C.

IPC: G01N21/55

CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G01N2021/6421 ,  G01N2021/6463 ,  G02B21/002 ,  G02B21/0076

Abstract: Provided is a coaxial illumination and collection laser scanning system designed to provide substantially uniform detection sensitivity across the field of view of an objective lens (27; 127) by reducing lateral chromatic aberrations at the expense of amplifying axial chromatic aberrations. Axial chromatic aberrations in the system are removed in the path of a retro-beam (31). A laser (18; 117, 118) is in optical communication with the objective lens. The laser (18; 117, 118) produces a collimated beam of coherent light (19; 119) that is directed by a scanner (25) through the objective lens (27; 127) to illuminate a raster of spots on the sample's surface (29), thereby stimulating a series of small regions of the sample to emit light.

Abstract translation: 提供了一种同轴照明和采集激光扫描系统，其被设计为通过以放大轴向色差为代价减少横向色差来提供物镜（27; 127）的视场的基本上均匀的检测灵敏度。 系统中的轴向色差在逆光束（31）的路径中被去除。 激光器（18; 117,118）与物镜光学连通。 激光器（18; 117,118）产生由扫描仪（25）引导通过物镜（27; 127）的相干光（19; 119）的准直光束，以照亮样品表面上的光斑 29），从而刺激样品的一系列小区域发光。

公开(公告)号：WO1997004354A1

公开(公告)日：1997-02-06

申请号：PCT/US1996006370

申请日：1996-05-06

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  MILLER, Michael, F. ,  MAJLOF, Lars ,  KAIN, Robert, C.

IPC: G03B42/00

CPC classification number: G01N21/6452 ,  G01J3/4406 ,  G01J2003/1213 ,  G01J2003/1221 ,  G02B26/10

Abstract: A compact, movable scan head (10) has multiple scanning modalities, capable of high speed and high resolution scanning of a variety of samples (20). Stimulation and detection of storage phosphor screens and fluorescent samples (20) are preferably achieved with first and second channels (81, 82) in an optical path of a first side (11) of the scan head (10). The first side (11) preferably has a laser diode light source (14). Reading of reflective and transmissive signals is also possible. A third channel (83) is available in the optical path of a second side (12) of the scan head (10). The third channel preferably provides LED (32) point scanning and reading of fluorescent, reflective and transmissive signals received from the sample (20). The various modalities of the scan head (10) have coincident optical paths.

Abstract translation: 紧凑的可移动扫描头（10）具有多种扫描模式，能够对各种样品（20）进行高速度和高分辨率的扫描。 存储荧光屏和荧光样品（20）的刺激和检测优选通过扫描头（10）的第一侧（11）的光路中的第一和第二通道（81,82）来实现。 第一侧（11）优选地具有激光二极管光源（14）。 读取反射和透射信号也是可能的。 第三通道（83）可用在扫描头（10）的第二侧（12）的光路中。 第三通道优选地提供从样品（20）接收的荧光，反射和透射信号的LED（32）点扫描和读取。 扫描头（10）的各种形式具有一致的光路。

公开(公告)号：WO1994011015A1

公开(公告)日：1994-05-26

申请号：PCT/US1993011021

申请日：1993-11-12

Applicant: MOLECULAR DYNAMICS, INC. ,  SCHLESINGER, David

Inventor： MOLECULAR DYNAMICS, INC.

IPC: A61K37/00

CPC classification number: C07K14/001 ,  A23L3/3463 ,  A23L3/3526 ,  A61K8/64 ,  A61K47/64 ,  A61K47/645 ,  A61K2800/57 ,  A61Q15/00 ,  C07K7/02

Abstract: Composition, methods of synthesis and uses of a peptide-based moiety for oral drug delivery, having the general formula: [X-A]2 - [B]2 -1 - C, wherein X is an amino acid blocking group; A is selected from the group consisting of at least one amino acid; B is selected from the group consisting of lysine, ornithine and combinations thereof; C is selected from the group consisting of a free carboxyl group, or a protected carboxamide (CONH2); and n is the number of cycles of addition of B, including the addition of various xenobiotics and endogenous drugs.

Abstract translation: 具有以下通式的组合物，合成方法和基于肽的部分用于口服药物递送的方法：[XA] 2 - [B] 2 -1-C，其中X是氨基酸封闭 组; A选自由至少一个氨基酸组成的组; B选自赖氨酸，鸟氨酸及其组合组成的组; C选自游离羧基或被保护的甲酰胺（CONH 2）; n是加入B的循环次数，包括加入各种异生物和内源性药物。