公开(公告)号：WO1997046319A1

公开(公告)日：1997-12-11

申请号：PCT/US1997009649

申请日：1997-06-03

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： MOLECULAR DYNAMICS, INC. ,  ROACH, David, J. ,  JOHNSTON, Richard, F.

IPC: B01L03/02

CPC classification number: B01L3/0262 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01L3/0248 ,  B01L3/0255 ,  B01L2300/0838 ,  C40B60/14 ,  G01N2035/1037 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A microspot deposition system (12) featuring a hollow cylindrical wall (26) extending from a closed end (28), terminating in an open end (30) and including a longitudinal gap (34) extending from the open end (30) toward the closed end (28) to allow the rapid exhaustion of the atmosphere and efficient cleaning within the cylindrical wall (26). The cylindrical wall (26) defines a lumen (32) with both the lumen (32) and the gap (34) adapted to facilitate capillary action of liquid in fluid communication therewith to form a meniscus (46) proximate to the open end (30). To facilitate deposition of liquid contained within the lumen (32), the gap (34) may be tapered so that it is narrowest proximate to the open end (30). The narrowed portion of the gap (34) results in a meniscus (46) having a reduced area to ensure preferential fluid flow toward the open end (30), which facilitates deposition via capillary action between the liquid in the lumen (32) and a working surface (52).

Abstract translation: 一种微孔沉积系统（12），其具有从封闭端（28）延伸的中空圆柱形壁（26），终止于开口端（30），并且包括从开口端（30）延伸的纵向间隙（34） 闭合端（28）以允许气氛的快速耗尽并且在圆柱形壁（26）内进行有效的清洁。 圆柱形壁（26）限定内腔（32），内腔（32）和间隙（34）都适于促进与其流体连通的液体的毛细作用，以形成靠近开口端（30）的弯月面（46） ）。 为了便于包含在内腔（32）内的液体的沉积，间隙（34）可以是锥形的，使得其在开口端（30）附近最窄。 间隙（34）的变窄的部分导致弯液面（46）具有减小的面积，以确保朝向开口端（30）的优先流体流动，这有利于通过管腔（32）中的液体和 工作面（52）。

公开(公告)号：EP1200191A1

公开(公告)日：2002-05-02

申请号：EP00952424.0

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： JOVANOVICH, Stevan, B. ,  ROACH, David, J. ,  HADD, Andrew, G. ,  HELLMAN, Bo, E., R.

IPC: B01L7/00 ,  B01L3/00

CPC classification number: C12Q1/6869 ,  B01J2219/00286 ,  B01J2219/00369 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00691 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/5025 ,  B01L7/52 ,  B01L2300/0838 ,  B01L2300/1844 ,  B01L2400/0409 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/6834 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/809 ,  Y10T436/113332 ,  Y10T436/143333 ,  Y10T436/2575 ,  C12Q2565/518 ,  C12Q2535/101 ,  C12Q2527/125

Abstract: An automated system utilizes an array of nanoscale capillary-dimension reaction chambers (12). The ends of the chambers are temporarily sealed with deformable membranes (264a, 264b) and the reactions effected by incubation or temperature cycling. The reaction containers may be filled by capillary action and dispensed by air displacement, centrifugal force or other means. Reaction mixtures may be assembled by using the reaction chambers (12) to meter reaction components that are combined on a substrate. Alternatively, a first reaction component may be immobilized on the interior surface of the reaction container and a second mixture component pumped of drawn into the container to form a final reaction mixture.

公开(公告)号：EP0991472A1

公开(公告)日：2000-04-12

申请号：EP97928828.9

申请日：1997-06-03

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： ROACH, David, J. ,  JOHNSTON, Richard, F.

IPC: B01L3/02 ,  G01N35/10

CPC classification number: B01L3/0262 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01L3/0248 ,  B01L3/0255 ,  B01L2300/0838 ,  C40B60/14 ,  G01N2035/1037 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A microspot deposition system (12) featuring a hollow cylindrical wall (26) extending from a closed end (28), terminating in an open end (30) and including a longitudinal gap (34) extending from the open end (30) toward the closed end (28) to allow the rapid exhaustion of the atmosphere and efficient cleaning within the cylindrical wall (26). The cylindrical wall (26) defines a lumen (32) with both the lumen (32) and the gap (34) adapted to facilitate capillary action of liquid in fluid communication therewith to form a meniscus (46) proximate to the open end (30). To facilitate deposition of liquid contained within the lumen (32), the gap (34) may be tapered so that it is narrowest proximate to the open end (30). The narrowed portion of the gap (34) results in a meniscus (46) having a reduced area to ensure preferential fluid flow toward the open end (30), which facilitates deposition via capillary action between the liquid in the lumen (32) and a working surface (52).