公开(公告)号：KR1020110087674A

公开(公告)日：2011-08-03

申请号：KR1020100007205

申请日：2010-01-27

Applicant: 가톨릭대학교 산학협력단

Inventor： 조영애 ,  오은주 ,  안현영 ,  신종철

IPC: C12N5/071 ,  C12N5/078 ,  C12N5/02

Abstract: PURPOSE: An ex vivo method for proliferating endothelial progenitor cells(EPCs) is provided to effectively proliferate EPCs and prevent or treat anginogenesis and ischemic diseases. CONSTITUTION: A method for proliferating EPCs comprises a step of culturing CD34+ cel fraction in a serum-free medium containing SCF(stem cell factor), FLT-3L(FLT-3 ligand), IL-3, and bFGF(basic fibroblast growth factor). The culture is a non-adhesive ex vivo culture. The EPCs are derived from bone marrow, peripheral blood, or umbilical cord blood.

Abstract translation: 目的：提供增强内皮祖细胞（EPCs）的离体方法，有效增殖EPCs，预防或治疗血管发生和缺血性疾病。 构成：增殖EPCs的方法包括在含有SCF（干细胞因子），FLT-3L（FLT-3配体），IL-3和bFGF（碱性成纤维细胞生长因子）的无血清培养基中培养CD34 +细胞级分的步骤 ）。 培养物是一种非粘性离体培养物。 EPCs来源于骨髓，外周血或脐带血。

公开(公告)号：KR1020110046911A

公开(公告)日：2011-05-06

申请号：KR1020090103622

申请日：2009-10-29

Applicant: 가톨릭대학교 산학협력단

Inventor： 조영애 ,  김현경

IPC: C07K5/10 ,  C07K7/08 ,  A61K38/08 ,  A61P35/00

Abstract: PURPOSE: A peptide having an activity of suppressing angiogenesis is provided to suppress tube formulation, cell adhesion, and cell migration. CONSTITUTION: A peptide for suppressing angiogenesis contains an amino acid sequence of sequence number 1, DGDA(Asp-Gly-Asp-Ala). The peptide has 4-14 amino acid sequences having DGDA(Asp-Gly-Asp-Ala) in an amino acid sequence encoding kringle 2 domain of TK1-2 protein of sequence number 2. The peptide is derived from kringle domain of tissue-type plasminogen activator. A composition for suppressing angiogenesis contains the peptide as an active ingredient. A drug for suppressing angiogenesis for preventing or treating disease caused by angiogenesis contains the composition as an active ingredient.

Abstract translation: 目的：提供具有抑制血管发生活性的肽，以抑制管制剂，细胞粘附和细胞迁移。 构成：用于抑制血管生成的肽含有序列号1的氨基酸序列DGDA（Asp-Gly-Asp-Ala）。 该肽具有编码序列号2的TK1-2蛋白的kringle 2结构域的氨基酸序列中具有DGDA（Asp-Gly-Asp-Ala）的4-14个氨基酸序列。该肽衍生自组织型的三角结构域 纤溶酶原激活剂。 用于抑制血管生成的组合物含有作为活性成分的肽。 用于抑制血管生成预防或治疗由血管发生引起的疾病的药物含有作为有效成分的组合物。

公开(公告)号：KR100855024B1

公开(公告)日：2008-08-28

申请号：KR1020080040936

申请日：2008-04-30

Applicant: 가톨릭대학교 산학협력단

Inventor： 조영애 ,  이상배

IPC: C07K14/00 ,  C12N15/09

Abstract: 본 발명은 크링글 도메인 1 및 2로 이루어진 비-글리코실화 재조합 단백질 에 관한 것으로, 보다 구체적으로 티슈 타입 플라스미노겐 활성인자로부터 유래된 것으로서 상기 활성인자의 117번째와 184번째 아미노산인 아스파라긴이 모두 글루타민으로 치환된 크링글 도메인 1 및 2로 이루어진 비-글리코실화 재조합 단백질에 관한 것이다. 본 발명의 재조합 단백질은 혈관내피 세포의 증식 및 이동을 억제하는 활성을 가지고 있으므로 혈관신생 억제제로서 매우 유용하다. 또한, 본 발명에 따른 제조방법은 진핵세포 발현 시스템을 이용하여 별도의 리폴딩 단계 없이 고수율로 재조합 단백질을 용이하게 수득할 수 있다.
크링글 도메인, 비-글리코실화, 혈관신생

公开(公告)号：KR1020040072141A

公开(公告)日：2004-08-18

申请号：KR1020030008100

申请日：2003-02-10

Applicant: 가톨릭대학교 산학협력단

Inventor： 조영애 ,  이수영

IPC: A61K38/17

CPC classification number: A61K38/49 ,  A61K9/0053 ,  Y10S514/824 ,  Y10S514/825

Abstract: PURPOSE: A composition for anti-angiogenesis and inhibiting cancer growth comprising kringle domain protein TK1-2 and use thereof are provided, thereby inhibiting growth of endothelial cell, inhibiting angiogenesis and inhibiting growth of cancer, so that it can be useful for prevention of disease caused by angiogenesis and cancer. CONSTITUTION: The composition for anti-angiogenesis and inhibiting cancer growth comprises kringle domain protein TK1-2 derived from human tissue type plasminogen activator(t-PA) and having the amino acid sequence set forth in SEQ ID NO: 1. A recombinant E. coli expression plasmid pET-15b/TK1-2 produces the kringle domain protein TK1-2. The recombinant Pichia expression plasmids His-TK1-2/pPICZα-C and N-TK1-2/pPICZα-C produce the kringle domain protein TK1-2. A transformed E. coli produced by transforming E. coli with the plasmid pET-15b/TK1-2. A transformed Pichia sp. (KCTC 10415BP) produced by transforming Pichia with N-TK1-2/pPICZα-C produces the protein TK1-2.

Abstract translation: 目的：提供用于抗血管发生和抑制癌生长的组合物，其包含三角结构域蛋白TK1-2及其用途，从而抑制内皮细胞的生长，抑制血管生成并抑制癌症生长，使其可用于预防疾病 由血管生成和癌症引起。 构成：用于抗血管发生和抑制癌症生长的组合物包括衍生自人组织型纤溶酶原激活剂（t-PA）并具有SEQ ID NO：1所示氨基酸序列的三环结构域蛋白TK1-2。 大肠杆菌表达质粒pET-15b / TK1-2产生三环结构域蛋白TK1-2。 重组毕赤酵母表达质粒His-TK1-2 /pPICZα-C和N-TK1-2 /pPICZα-C产生三环结构域蛋白TK1-2。 通过用质粒pET-15b / TK1-2转化大肠杆菌产生的转化的大肠杆菌。 转化毕赤酵母 （KCTC 10415BP）通过用N-TK1-2 /pPICZα-C转化毕赤酵母产生蛋白质TK1-2。