-
公开(公告)号：KR1020080053256A
公开(公告)日：2008-06-12
申请号：KR1020080040936
申请日：2008-04-30
Applicant: 가톨릭대학교 산학협력단
Abstract: A non-glycosylated recombinant protein comprising kringle domains 1 and 2 is provided to inhibit angiogenesis by inhibiting growth and mobility of vascular endothelial cells, and improve preparation yield of recombinant protein by removing a refolding step through eukaryotic cell expression system. A non-glycosylated recombinant protein comprises kringle domains 1 and 2 derived from human tissue type plasminogen activation factor(t-PA), in which 117 and 184 amino acids of t-PA are substituted from asparagine to glutamine, and has the amino acid sequence of SEQ ID NO:4. A gene encoding the non-glycosylated recombinant protein has the nucleotide sequence of SEQ ID NO:5. A Pichia pastoris(KCTC 11036BP) expresses the non-glycosylated recombinant protein comprising kringle domains 1 and 2.
Abstract translation: 提供了包含三环结构域1和2的非糖基化重组蛋白，通过抑制血管内皮细胞的生长和迁移性来抑制血管生成，并通过通过真核细胞表达系统去除重折叠步骤来提高重组蛋白的制备产率。 非糖基化重组蛋白包含衍生自人组织型纤溶酶原激活因子（t-PA）的三环结构域1和2，其中t-PA的117和184个氨基酸被天冬酰胺取代为谷氨酰胺，并且具有氨基酸序列 的SEQ ID NO：4。 编码非糖基化重组蛋白的基因具有SEQ ID NO：5的核苷酸序列。 巴斯德毕赤酵母（KCTC 11036BP）表达包含三环结构域1和2的非糖基化重组蛋白。
-
公开(公告)号：KR100834561B1
公开(公告)日：2008-06-02
申请号：KR1020060124283
申请日：2006-12-08
Applicant: 가톨릭대학교 산학협력단
Abstract: A method for preparing a non-glycosylated recombinant protein is provided to obtain the recombinant protein easily with high yield without a refolding step using an eukaryotic expression system. A recombinant protein prepared by the method is provided to inhibit proliferation and movement of endothelial cells, thereby being very useful as an angiogenesis inhibitor. A method for preparing a non-glycosylated recombinant protein consisting of a kringle domain 1 and a kringle domain 2 comprises the steps of: (a) culturing Pichia pastoris(deposition no. KCTC 11038BP) which includes an amino acid sequence described as SEQ ID : NO. 4, has the angiogenesis inhibitory activity and expresses the non-glycosylated recombinant protein; and (b) subjecting the culture material of the step(a) to ion chromatography using an FPLC(fast protein liquid chromatography) column to isolate and purify the recombinant protein. A pharmaceutical composition for inhibiting the angiogenesis comprises the non-glycosylated recombinant protein derived from Pichia sp. as an effective ingredient.
Abstract translation: 提供了制备非糖基化重组蛋白的方法，以高产率容易地获得重组蛋白而无需使用真核表达系统的重折叠步骤。 提供了通过该方法制备的重组蛋白质以抑制内皮细胞的增殖和运动，从而作为血管生成抑制剂非常有用。 制备由三环结构域1和三环结构域2组成的非糖基化重组蛋白质的方法包括以下步骤：（a）培养巴斯德毕赤酵母（沉积编号KCTC 11038BP），其包含SEQ ID NO： 没有。 4，具有血管生成抑制活性，并表达非糖基化重组蛋白; 和（b）使用FPLC（快速蛋白质液相色谱）柱对步骤（a）的培养物进行离子色谱以分离和纯化重组蛋白。 用于抑制血管发生的药物组合物包含衍生自毕赤酵母属的非糖基化重组蛋白。 作为有效成分。
-
公开(公告)号：KR100855024B1
公开(公告)日：2008-08-28
申请号：KR1020080040936
申请日：2008-04-30
Applicant: 가톨릭대학교 산학협력단
Abstract: 본 발명은 크링글 도메인 1 및 2로 이루어진 비-글리코실화 재조합 단백질 에 관한 것으로, 보다 구체적으로 티슈 타입 플라스미노겐 활성인자로부터 유래된 것으로서 상기 활성인자의 117번째와 184번째 아미노산인 아스파라긴이 모두 글루타민으로 치환된 크링글 도메인 1 및 2로 이루어진 비-글리코실화 재조합 단백질에 관한 것이다. 본 발명의 재조합 단백질은 혈관내피 세포의 증식 및 이동을 억제하는 활성을 가지고 있으므로 혈관신생 억제제로서 매우 유용하다. 또한, 본 발명에 따른 제조방법은 진핵세포 발현 시스템을 이용하여 별도의 리폴딩 단계 없이 고수율로 재조합 단백질을 용이하게 수득할 수 있다.
크링글 도메인, 비-글리코실화, 혈관신생
-
-
Search Results
3
records
(took 0.021 seconds)
