Abstract:
An extracorporeal perfusion method of transplanted organs is provided to exclude surgical elements due to transplantation operations and to study ischemia without expert skill about the transplantation of organs by developing a reperfusion model using the artery and vein of the thigh. In an organ transplantation method for extracting organs of the animal, cleaning the extracted organs, and storing the organs at low temperature in long-term preserving fluid, an extracorporeal perfusion method of the transplanted organs in the animal comprises a step of separating the artery and vein of the thigh of the animal and a step of connecting the artery and vein of the extracted organ to the artery and vein of the thigh by using a catheter.
Abstract:
The present invention relate to a skin transplantation method for facilitating a collection of skin tissue comprising transplants when transplanting an animal skin. When using the skin transplantation method according to the present invention, a graft is firmly fixed to an implant site whereby risk of loss is small; and abundant samples can be acquired from a small number of animals, thereby enabling an effective application in research of transplant rejection in animals, etc.
Abstract:
PURPOSE: A method for removing heterologous immunogens and retrovirus from a bioscaffold is provided to prevent acute immune rejection or infection of retrovirus. CONSTITUTION: A method for removing heterologous immunogens and retrovirus from a bioscaffold comprises the following steps: preparing an internal organ to make a bioscaffold; and dipping the internal organ in a surfactant. The internal organ is the liver of a pig. The surfactant is 0.1-0.25% concentration of sodium dodecyl sulfate.
Abstract:
PURPOSE: A manufacturing method of bio scaffold is provided to remove major heteroimmunity antigen and manhole cask virus pathogen. CONSTITUTION: A manufacturing method of bio scaffold comprises the following steps: preparing an organ which will be manufactured into bio scaffold; and dipping the organ into SDS (sodium dodecyl sulfate). The organ is selected from a group consisting of anthropoid, pig, cow, sheep, and goat. When the organ is belong to pig, liver is used. The concentration of the SDS is 0.05-0.25 %.