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公开(公告)号：KR1020120052667A
公开(公告)日：2012-05-24
申请号：KR1020100113932
申请日：2010-11-16
Applicant: 대한민국(관리부서:국립수산과학원) , 씨제이제일제당 (주)
CPC classification number: A61K35/742 , A23K10/18 , A23K50/80 , C12R1/07
Abstract: PURPOSE: A novel Bacillus strain with a probiotic activity is provided to ensure antibacterial activity to fish pathogenic bacteria and to secrete useful enzymes. CONSTITUTION: A novel Bacillus 2-4(KCCM11107P) strain has a probiotic activity. A probiotic formulation contains the strain as an active ingredient. A feed or feed additive for cultivating fishes and/or crustaceans contains the strain as an active ingredient. A method for preventing mass mortality caused by pathogen of cultured fishes and/or crustaceans comprises a step of feeding the feed to the fishes and/or crustaceans. The pathogen includes Edwardsiella tarda, Streptococcus iniae, Vibrio anguillarum, Vibrio harveyi or Streptococcus parauberis.
Abstract translation: 目的：提供一种具有益生菌活性的新型芽孢杆菌菌株，以确保对病原菌的抗菌活性，并分泌有用的酶。 构成：一种新型的芽孢杆菌2-4（KCCM11107P）菌株具有益生菌活性。 益生菌制剂含有作为活性成分的菌株。 用于培养鱼类和/或甲壳类动物的饲料或饲料添加剂含有该菌株作为活性成分。 用于预防由鱼类和/或甲壳类动物的病原体引起的大量死亡的方法包括将饲料喂入鱼类和/或甲壳类动物的步骤。 病原体包括Edwardsiella tarda，Streptococcus iniae，Vibrio anguillarum，Vibrio harveyi或Streptococcus parauberis。
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公开(公告)号：KR1020110071057A
公开(公告)日：2011-06-28
申请号：KR1020110057469
申请日：2011-06-14
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: PURPOSE: A novel gene encoding Paralichthys olivaceus-derived beta-defensin is provided to ensure high antibacterial activity and to develop natural antibiotics. CONSTITUTION: A Paralichthys olivaceus-derived antibacterial peptide is a polymepeptide, mutant thereof, or active fragment thereof. The polypeptide is selected from: a polypeptide encoded by cDNA having one of nucleic acids of sequence number s 1-5; a polypeptide encoding a polynucleotide which is hybridized with cDNA having the one of nucleic acids of sequence numbers 1-5; a polypeptide encoded by gDNA having one or nucleic acids of sequence numbers 6-10; a polypeptide encoding the polynucleotide hybridized with the gDNA; or a polypeptide having an amino acid among amino acid sequences of sequence numbers 11-15.
Abstract translation: 目的：提供一种编码Paralichthys olivaceus来源的β-防御素的新基因，以确保高抗菌活性和开发天然抗生素。 构成：来自Paralichthys olivaceus的抗菌肽是多肽，其突变体或其活性片段。 所述多肽选自：由具有序列号为1-5的核酸之一的cDNA编码的多肽; 编码与序列号为1-5的核酸的cDNA杂交的多核苷酸的多肽; 由具有序列号6-10的一个或核酸的gDNA编码的多肽; 编码与所述gDNA杂交的多核苷酸的多肽; 或在序列号11-15的氨基酸序列中具有氨基酸的多肽。
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公开(公告)号：KR100540071B1
公开(公告)日：2006-01-11
申请号：KR1020030053096
申请日：2003-07-31
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: A01H4/00
Abstract: 본 발명은 참지누아리의 포자로부터 종묘를 생산하는 방법에 관한 것으로서, 보다 상세하게는 1) 참지누아리의 성숙엽체를 효율적인 포자 방출을 위하여 음건시킴으로써 포자를 대량으로 방출시켜 종사에 부착시키는 단계; 2) 종사에 부착된 포자를 배양하여 포자의 반상근을 형성하게 하는 단계; 및 3) 반상근이 부착된 종사를 배양하여 반상근을 성장시키는 단계를 포함하는 참지누아리의 포자로부터 종묘를 생산하는 방법에 관한 것이다. 본 발명의 참지누아리의 종묘 생산 방법은 자연 생산량에만 의존하고 최근에는 남획 등으로 인하여 자원량이 급속히 감소하고 있는 참지누아리의 대량 양식에 유용하게 사용될 수 있다.
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公开(公告)号：KR101703540B1
公开(公告)日：2017-02-08
申请号：KR1020130052956
申请日：2013-05-10
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: C12N15/70
Abstract: 본발명은단백질의발현을조절하기위하여, 1) 프로모터; 2) 번역종결코돈을포함하는외래단백질을암호화하는폴리뉴클레오티드의삽입을위한제한효소인식부위; 및 3) 트리뉴클레오티드및 헥사뉴클레오티드의반복체가순차적으로연결된유전자컨스트럭트를포함하는, 활성외래단백질의전체발현을조절하기위한발현벡터를제공한다.
Abstract translation: 本发明提供了用于控制活性异源蛋白质的总体表达的表达载体，其包括：1）启动子; 2）用于插入编码包含翻译终止密码子的异源蛋白质的多核苷酸的限制性位点; 和3）依次结合三核苷酸和六核苷酸重复的基因构建体。
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公开(公告)号：KR101508693B1
公开(公告)日：2015-04-07
申请号：KR1020130120993
申请日：2013-10-11
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C07K14/435 , C12N15/12 , A61K38/17 , A23K20/195
Abstract: 본발명은항미생물성펩타이드에관한것으로서, 어류병원성미생물에대하여효율적인항미생물활성을나타낼뿐만아니라, 적혈구에대한용혈활성은거의없기때문에, 양식어류에대한사료첨가제로사용되거나, 항생제대체제로사용되더라도어류및 인체의건강에대한위협요소를제공하지않는다.
Abstract translation: 本发明涉及一种抗菌肽，其具有对来自鱼类的病原微生物有效的抗微生物活性，对红细胞无溶血活性，因此可用作养鱼的饲料添加剂，并且对人和鱼无害，即使 抗菌肽用作抗生素替代品。
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Patent Agency Ranking
公开(公告)号：KR101049859B1
公开(公告)日：2011-07-19
申请号：KR1020080035162
申请日：2008-04-16
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: C12N15/62 , C12N15/625
Abstract: 본 발명은 pI값이 조절된 신호서열(directional signal)의 N-영역을 포함하는 폴리펩타이드 단편 또는 이의 변이체 및/또는 pI값이 조절된 친수성 폴리펩타이드로 구성된 분비증강자를 이용한 재조합 단백질의 분비효율 향상방법에 관한 것이다. 본 발명의 방법은 재조합 단백질의 불용성 침전을 방지하고, 세포질 밖 또는 페리플라즘으로의 재조합 단백질의 분비 효율을 향상시킴으로써, 재조합 외래단백질의 생산에 유용하게 사용할 수 있을 뿐만 아니라, 강력한 분비증강자를 이용하여 막 침투성을 향상시켜, 유용 치료용 단백질의 이동(transduction)에 이용될 수 있다.
재조합 단백질, N-말단 pI값, MefpI, 헵시딘Abstract translation: 本发明提高使用字符分泌增强剂的重组蛋白由多肽片段的分泌效率或它们的变体和/或与含有的pI值的信号序列的N区的pI一个值控制的亲水性多肽进行调整（定向信号） ＆lt; 本发明的方法中，通过防止重组蛋白的水不溶性的沉淀物，并提高重组蛋白的分泌效率的外细胞质或周质，以及在生产重组外源蛋白的，使用这些强分泌增强子的有用 由此改善膜的渗透性，并且可以用于转导有用的治疗性蛋白质。
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公开(公告)号：KR1020110048746A
公开(公告)日：2011-05-12
申请号：KR1020090105447
申请日：2009-11-03
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: PURPOSE: A novel Photobacterium gaetbulicola Gung47 which produces lipase is provided to be used as a detergent, bleaching agent, food, and feed additive. CONSTITUTION: A novel Photobacterium gaetbulicola Gung47 which produces lipase is deposited by deposit number KACC 91494P. The Photobacterium gaetbulicola strain has 16S rRNA of sequence number 3. The molecular weight of the lipase is 100 kDa. The Photobacterium gaetbulicola strain grows at 10°C-40°C and pH 5.0 to pH 9.5 and under anaerobic condition. A feed additive contains the Photobacterium gaetbulicola Gung47, culture liquid, and lipase as an active ingredient.
Abstract translation: 目的：提供生产脂肪酶的新型光杆菌Gung47，用作洗涤剂，漂白剂，食品和饲料添加剂。 构成：产生脂肪酶的新型光杆菌Gung47由保藏号KACC 91494P保藏。 光杆菌菌株具有序列号3的16S rRNA。脂肪酶的分子量为100kDa。 光杆菌菌株在10℃-40℃，pH5.0至pH9.5和无氧条件下生长。 饲料添加剂含有光杆菌Gung47，培养液和脂肪酶作为活性成分。
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公开(公告)号：KR101618220B1
公开(公告)日：2016-05-18
申请号：KR1020140020365
申请日：2014-02-21
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: 항균활성및 프로바이오틱스활성을갖는신규바실러스속균주가제공된다. 본발명의균주는다양한어류병원성세균뿐만아니라, 화장품표준균주및 치아우식균에대한광범위한항균활성을가지고있을뿐만아니라, 프로테아제, 아밀라제, 셀룰라제, 자일라제등 사료의소화흡수에도움을주는유용한효소를분비하는등 프로바이오틱스로서기능을하기때문에, 어류및 갑각류의사료첨가제는물론, 항균제와식품및 화장품분야의보존용첨가제로유용하게사용될수 있다.
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公开(公告)号：KR101395942B1
公开(公告)日：2014-05-15
申请号：KR1020130030496
申请日：2013-03-21
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: Y02A50/473
Abstract: The present invention relates to antimicrobial peptides, and provides an antibiotic peptide having a length of more than 12 amino acids selected from the amino acid sequence of SEQ ID: 3 and comprising the amino acid sequences of SEQ ID: 21 and uses thereof. According to an aspect of the present invention, a polynucleotide encoding an antimicrobial peptide according to an embodiment of the present invention is provided. In addition, a vector comprising a polynucleotide according to an embodiment of the present invention is provided.
Abstract translation: 本发明涉及抗微生物肽，并提供长度多于12个氨基酸的抗生素肽，其选自SEQ ID：3的氨基酸序列并且包含SEQ ID：21的氨基酸序列及其用途。 根据本发明的一个方面，提供了编码本发明实施方案的抗微生物肽的多核苷酸。 此外，提供了包含根据本发明实施方案的多核苷酸的载体。
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公开(公告)号：KR1020090055457A
公开(公告)日：2009-06-02
申请号：KR1020080035162
申请日：2008-04-16
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: C12N15/62 , C12N15/625
Abstract: A method for producing a water soluble recombinant fusion protein from an exogenous gene and collecting a protein having an original amino terminal is provided to prevent insoluble precipitation of the recombinant protein and improve the secretion efficiency of the recombinant protein to cytoplasm outside or periplasm. A method for improving the secretion efficiency of a foreign protein comprises: a step of constructing a leader sequence containing N-region of the signal sequence and/or leader sequence of foreign protein, of which pI value is 9.90-11.28; a step of adjusting the distance of amino acid which makes an impact on pI value in the leader sequence; a step of constructing the leaser sequence, distance adjusting site, restriction enzyme recognition site and a polynucleotide coding fusion protein containing foreign protein; a step of inserting the constructed gene into an expression vector to produce a recombinant expression vector; a step of transforming a host cell with the recombinant expression vector; and a step of screening a transformant having high water-soluble expression.
Abstract translation: 提供从外源基因产生水溶性重组融合蛋白并收集具有原始氨基末端的蛋白质的方法，以防止重组蛋白的不溶性沉淀，并提高重组蛋白在细胞质外周或周质中的分泌效率。 提高外源蛋白质分泌效率的方法包括：构建含有pI值为9.90-11.28的信号序列的N-区和/或外来蛋白的前导序列的前导序列的步骤; 调整导致序列中对pI值产生影响的氨基酸的距离的步骤; 构建leaser序列，距离调整位点，限制酶识别位点和含有外源蛋白的多核苷酸编码融合蛋白的步骤; 将构建的基因插入表达载体以产生重组表达载体的步骤; 用重组表达载体转化宿主细胞的步骤; 以及筛选具有高水溶性表达的转化体的步骤。
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