公开(公告)号：KR1020000073245A

公开(公告)日：2000-12-05

申请号：KR1019990016428

申请日：1999-05-08

Applicant: 대한민국(농촌진흥청장)

Inventor： 김삼은 ,  조세연 ,  최지영 ,  지상덕 ,  김종길 ,  임수호

IPC: A01G1/04

CPC classification number: C12N1/04 ,  A01K67/033

Abstract: PURPOSE: An artificial cultivation method of Cordyceps using Nd mutant silkworm instead of silkworm is provided to increase the output with reduced labor and improve the fruit body forming rate. CONSTITUTION: The method comprises the following steps: (i) diluting a spore of Paecilomyces japonica to 107-108 spores/ml; (ii) inoculating the diluted spore to a host silkworm in the larval stage which cast off the skin 4times by spraying; (iii) leaving the host at 26-30°C under 95% of relative humidity for 12hr and breeding with mulberry leaves; and then (iv) if infections is confirmed, forming a fruit body by protecting the host at 21-25°C under 95% of relative humidity. The first hybrid of Nd mutant silkworm is used for the host.

Abstract translation: 目的：提供使用Nd突变体蚕代替蚕的冬虫夏草的人造栽培方法，以减少劳动力提高产量，提高果实体形成率。 构成：该方法包括以下步骤：（i）将拟青孢子孢子稀释至107-108孢子/ ml; （ii）将稀释的孢子接种在幼虫阶段，通过喷洒4次皮肤脱落皮肤的宿主蚕; （iii）将主体在26-30℃下在95％相对湿度下离心12小时，并用桑叶进行育种; 然后（iv）如果感染被证实，通过在相对湿度95％的21-25℃下保护宿主形成水果体。 Nd突变体蚕的第一个杂交体用于宿主。

公开(公告)号：KR100187895B1

公开(公告)日：1999-06-01

申请号：KR1019960062055

申请日：1996-12-05

Applicant: 대한민국(농촌진흥청장)

Inventor： 조세연 ,  지상덕 ,  임수호

IPC: A01G1/04

Abstract: 본 발명은 항암·면역증강 등 각종 효능이 뛰어난 것으로 알려져 있으나 자연에서의 채집이 극히 어려운 관계로 인간에게 유용하게 이용되지 못하고 있는 동충하초(Paecilomyces sp. J300)를 인공재배하는 방법에 관한 것으로, 동충하초의 일종인 페실로마이세스속 균주 J300의 포자를 분무기를 이용하여 가잠의 5령기 유충 표피에 경피 접종한 후, 균사체를 형성한 번데기에서 자실체를 형성시키는 것을 특징으로 한다.

公开(公告)号：KR1019980025197A

公开(公告)日：1998-07-06

申请号：KR1019970065094

申请日：1997-12-01

Applicant: 대한민국(농촌진흥청장)

Inventor： 지상덕 ,  임수호 ,  조세연

IPC: A01G1/04

Abstract: 본 발명은 항암·면역증강 등 각종 효능이 뛰어난 것으로 알려져 있으나, 자연에서의 채집이 극히 어려운 관계로 인간에게 유용하게 이용되지 못하고 있는 동충하초(Paecilomyces farinosa)를 인공재배하는 방법에 관한 것으로, 동충하초의 일종인 번데기 봉형 눈꽃동충하초 균주의 포자를 가잠의 5령기 유충 표피에 경피 접종한 후, 감염되어 균사체를 형성한 번데기에서 자실체를 형성시키는 것을 특징으로 한다.

公开(公告)号：KR1020050002704A

公开(公告)日：2005-01-10

申请号：KR1020040095669

申请日：2004-11-22

Applicant: 문창현 ,  대한민국(농촌진흥청장)

Inventor： 안미영 ,  조세연 ,  지상덕 ,  이용기 ,  문창현 ,  정이숙 ,  이보경 ,  김찬식

IPC: A23L1/29

CPC classification number: A23L33/105 ,  A23V2002/00 ,  A23V2200/326 ,  A23V2250/21

Abstract: PURPOSE: A food composition containing Isaria sinclairii(Paecilomyces cicadae) as an effective ingredient is provided. It is useful for preventing or improving disease selected from hypertension, cerebral apoplexy, arteriosclerosis, coronary artery disease, myocardial infarction mediated rise in blood pressure. CONSTITUTION: The food composition for reducing blood pressure contains Isaria sinclairii, a water soluble extract or an alcohol extract. The Isaria sinclairii is selected from infected silkworms, infected pupae, fruit body-forming pupae, artificial or natural cultures. The pharmaceutical composition for reducing blood pressure contains Isaria sinclairii, a water soluble extract or an alcohol extract and a pharmaceutically acceptable carrier. The daily dosage level of the composition is 1 to 10g in single or divided doses.

Abstract translation: 目的：提供含有Isaria sinclairii（Paecilomyces cicadae）作为有效成分的食品组合物。 可用于预防或改善选自高血压，脑中风，动脉硬化，冠状动脉疾病，心肌梗死介导的血压升高的疾病。 构成：用于降低血压的食物组合物含有Isaria sinclairii，水溶性提取物或酒精提取物。 Isaria sinclairii选自感染的蚕，感染蛹，果实体形蛹，人造或天然培养物。 用于降低血压的药物组合物含有Isaria sinclairii，水溶性提取物或醇提取物和药学上可接受的载体。 组合物的日剂量水平为单剂量或分剂量为1至10g。

公开(公告)号：KR100434843B1

公开(公告)日：2004-06-07

申请号：KR1020010018665

申请日：2001-04-09

Applicant: 대한민국(농촌진흥청장) ,  학교법인 한동대학교

Inventor： 조세연 ,  지상덕 ,  송성규 ,  이관희

IPC: C07D207/12

Abstract: PURPOSE: Provided are 4-methyl-2-((pyrrolidine-2-carbonyl)-2-amino)-pentanoic acid extracted and purified from the J300 Cordyceps militaris, and a pharmaceutical composition having anti-HIV activity, an agent for treating AIDS and a supplementary food containing the same as an active ingredient. CONSTITUTION: The 4-methyl-2-((pyrrolidine-2-carbonyl)-2-amino)-pentanoic acid is manufactured by the steps of: extracting Cordyceps militaris powders with water; performing HPLC with the extract using distilled water; isolating the distilled water fraction by HPLC using methanol; and isolating the methanol fraction by activated carbon chromatography to obtain J300 Cordyceps militaris water extract Dong1-2-5 having anti-HIV activity.

Abstract translation: 目的：本发明提供了4-甲基-2 - （（吡咯烷-2-羰基）-2-氨基） - 戊酸萃取并​​从J300蛹虫草纯化，并具有抗HIV活性的药物组合物的药剂，用于治疗AIDS 和含有其作为活性成分的补充食品。 组成：4-甲基-2 - （（吡咯烷-2-羰基）-2-氨基） - 戊酸通过以下步骤制备：用水提取蛹虫草粉末; 用蒸馏水对提取物进行HPLC; 用甲醇通过HPLC分离蒸馏水部分; 并通过活性炭层析分离甲醇部分，得到具有抗HIV活性的J300北冬虫夏草水提物Dong1-2-5。

公开(公告)号：KR1020040012396A

公开(公告)日：2004-02-11

申请号：KR1020020045966

申请日：2002-08-03

Applicant: 대한민국(농촌진흥청장) ,  이강노

Inventor： 조세연 ,  정이연 ,  이강노 ,  권학철 ,  김선녀

IPC: C07C233/16

Abstract: PURPOSE: Provided is a beauveria bassiana 101A extract containing an active compound which has excellent activity in nerve cell outgrowth without cytotoxicity. The extract and active compound separated therefrom can reduce atrophy and damages caused by degenerative cerebral diseases including Parkinson's disease and stroke. CONSTITUTION: The active compound from beauveria bassiana extract is represented by formula 1, wherein R is C17 or C19 of alkyl group. The pharmaceutical composition using the active compound from beauveria bassiana extract for treatment or prophylaxis of cerebral diseases comprises one or more selected from a group consisting of 1,7-dimethyl-xanthine, uracil, urea, betain, and tyrosine, as an active compound.

Abstract translation: 目的：本发明提供一种含有活性化合物的白僵菌101A提取物，其在没有细胞毒性的神经细胞生长中具有优异的活性。 从其分离的提取物和活性化合物可以减少由退行性脑疾病（包括帕金森病和中风）引起的萎缩和损伤。 构成：来自白僵菌提取物的活性化合物由式1表示，其中R为烷基的C17或C19。 使用白僵菌提取物的活性化合物用于治疗或预防脑疾病的药物组合物包含选自1-7-二甲基 - 黄嘌呤，尿嘧啶，尿素，甜菜碱和酪氨酸作为活性化合物的一种或多种。

公开(公告)号：KR100416912B1

公开(公告)日：2004-02-05

申请号：KR1020010018664

申请日：2001-04-09

Applicant: 대한민국(농촌진흥청장) ,  학교법인 한동대학교

Inventor： 조세연 ,  지상덕 ,  송성규 ,  이관희

IPC: C07D241/08

Abstract: PURPOSE: Provided are 3-(5-(methoxy-ethyl)-3,6-dioxo-piperazine-2-yl)-propionic acid of the formula(1) extracted and purified from the J300 Cordyceps militaris, and a pharmaceutical composition having anti-HIV activity, an agent for treating AIDS and a supplementary food containing the same. CONSTITUTION: The 3-(5-(methoxy-ethyl)-3,6-dioxo-piperazine-2-yl)-propionic acid of the formula(1) is manufactured by the steps of: extracting Cordyceps militaris powders with water; performing HPLC with the extract using distilled water; isolating the distilled water fraction by HPLC using methanol; and isolating the methanol fraction by activated carbon chromatography to obtain J300 Cordyceps militaris water extract Dong1-2-4 having anti-HIV activity.

Abstract translation: 用途：提供从J300蛹虫草中提取和纯化的式（1）的3-（5-（甲氧基 - 乙基）-3,6-二氧代 - 哌嗪-2-基） - 丙酸和一种药物组合物， 抗HIV活性，治疗艾滋病的药剂和含有它的补充食品。 构成：式（1）的3-（5-（甲氧基 - 乙基）-3,6-二氧代 - 哌嗪-2-基） - 丙酸通过以下步骤制备：用水提取蛹虫草粉末; 用蒸馏水对提取物进行HPLC; 用甲醇通过HPLC分离蒸馏水部分; 并通过活性炭层析分离甲醇部分，得到具有抗HIV活性的J300北冬虫夏草水提物Dong1-2-4。

公开(公告)号：KR1020030078549A

公开(公告)日：2003-10-08

申请号：KR1020020017665

申请日：2002-03-30

Applicant: 문창현 ,  대한민국(농촌진흥청장)

Inventor： 안미영 ,  조세연 ,  지상덕 ,  이용기 ,  문창현 ,  정이숙 ,  이보경 ,  김찬식

IPC: A61K36/066 ,  A61P9/12

Abstract: PURPOSE: Provided is a composition comprising Isaria sinclairii itself, and its water soluble extract or alcohol extract as an active ingredient, thereby reducing blood pressure and body weight. CONSTITUTION: A composition for decreasing blood pressure is characterized by containing Isaria sinclairii itself, and its water soluble extract or alcohol extract as an active ingredient. It is also capable of being applied to health foods.

Abstract translation: 目的：提供包含Isaria sinclairii本身及其水溶性提取物或醇提取物作为活性成分的组合物，从而降低血压和体重。 构成：用于降低血压的组合物的特征在于含有Isaria sinclairii本身，其水溶性提取物或醇提取物作为活性成分。 它也可以应用于保健食品。

公开(公告)号：KR100382166B1

公开(公告)日：2003-04-26

申请号：KR1020010007899

申请日：2001-02-16

Applicant: 대한민국(농촌진흥청장)

Inventor： 남성희 ,  조세연 ,  정이연 ,  지상덕 ,  최영숙

IPC: A01G1/04

Abstract: PURPOSE: Provided is a preparation method of a medium for Entomopathogenic fungi effectively and economically by using silkworm powder used as a hypoglycemic agent. The medium increases the growth rate of Entomopathogenic fungi. CONSTITUTION: The medium for Entomopathogenic fungi is characterized by dissolving 24g of silkworm powder in 1L of distilled water; sterilizing it at 121 deg.C for 15 minutes; filtering the sterilized solution with gauze and a sieve sequentially; adding 15g of agar the pure solution; sterilizing it; and pipetting 21mL of the solution into each petridish the solidifying them.

Abstract translation: 用途：本发明提供一种利用蚕粉作为降血糖剂有效经济地制备用于昆虫病原真菌的培养基的方法。 培养基增加了Entomopathogenic真菌的生长速度。 构成：昆虫病原真菌的培养基的特征是将24g家蚕粉溶于1L蒸馏水中; 在121℃灭菌15分钟; 依次用纱布和筛子过滤灭菌的溶液; 加入15g琼脂纯溶液; 消毒; 并将21mL溶液移入每个培养皿中使其固化。

公开(公告)号：KR1020020078323A

公开(公告)日：2002-10-18

申请号：KR1020010018665

申请日：2001-04-09

Applicant: 대한민국(농촌진흥청장) ,  학교법인 한동대학교

Inventor： 조세연 ,  지상덕 ,  송성규 ,  이관희

IPC: C07D207/12

Abstract: PURPOSE: Provided are 4-methyl-2-((pyrrolidine-2-carbonyl)-2-amino)-pentanoic acid extracted and purified from the J300 Cordyceps militaris, and a pharmaceutical composition having anti-HIV activity, an agent for treating AIDS and a supplementary food containing the same as an active ingredient. CONSTITUTION: The 4-methyl-2-((pyrrolidine-2-carbonyl)-2-amino)-pentanoic acid is manufactured by the steps of: extracting Cordyceps militaris powders with water; performing HPLC with the extract using distilled water; isolating the distilled water fraction by HPLC using methanol; and isolating the methanol fraction by activated carbon chromatography to obtain J300 Cordyceps militaris water extract Dong1-2-5 having anti-HIV activity.

Abstract translation: 目的：提供从J300冬虫夏草提取和纯化的4-甲基-2 - （（吡咯烷-2-羰基）-2-氨基） - 戊酸和具有抗HIV活性的药物组合物，用于治疗AIDS 和含有与活性成分相同的补充食品。 构成：4-甲基-2 - （（吡咯烷-2-羰基）-2-氨基） - 戊酸通过以下步骤制造：用水提取冬虫夏草粉末; 使用蒸馏水用提取物进行HPLC; 使用甲醇通过HPLC分离蒸馏水部分; 并通过活性炭色谱分离甲醇馏分，得到具有抗HIV活性的J300冬虫夏草水提取物Dong1-2-5。