公开(公告)号：KR1020120038576A

公开(公告)日：2012-04-24

申请号：KR1020100100091

申请日：2010-10-14

Applicant: 대한민국(농촌진흥청장)

Inventor： 김둘이 ,  윤인선 ,  변명옥 ,  신동진 ,  한세연

IPC: A01H5/00 ,  C12N15/82 ,  C12N15/11

CPC classification number: C12N15/8273 ,  A01H4/008 ,  C07K14/415 ,  C12N15/8205 ,  C12N15/8216

Abstract: PURPOSE: A disaster-inducible pDREB1 promoter derived from potato and an expression vector containing the promoter are provided to efficiently produce crops. CONSTITUTION: A disaster-inducible pDREB1 promoter contains a sequence of sequence number 1. The promoter is derived from potato. The promoter is isolated using a primer set of a sense primer, pDREB1-S(pDREB1-S : 5-TGCGTTACATGTTGGTACCA-3') and an antisense primer, pDREB1-AS(pDREB1-AS: 5-TGTAACAAGGCAAAACACTCA-3'). An expression vector for plant transformation contains the pDREB1 promoter. A method for producing a transgenic plant comprises: a step of preparing the plant expression vector; a step of transforming plant cells with the expression vector; and a step of redifferentitating the transgenic plant.

Abstract translation: 目的：提供源自马铃薯的灾难诱导型pDREB1启动子和含有启动子的表达载体，以有效生产作物。 构成：灾害诱导型pDREB1启动子含有序列号1的序列。启动子来源于马铃薯。 使用有义引物pDREB1-S（pDREB1-S：5-TGCGTTACATGTTGGTACCA-3'）和反义引物pDREB1-AS（pDREB1-AS：5-TGTAACAAGGCAAAACACTCA-3'）的引物组分离启动子。 植物转化的表达载体含有pDREB1启动子。 一种生产转基因植物的方法包括：制备植物表达载体的步骤; 用表达载体转化植物细胞的步骤; 以及重新分化转基因植物的步骤。

公开(公告)号：KR1020090121131A

公开(公告)日：2009-11-25

申请号：KR1020080047271

申请日：2008-05-21

Applicant: 대한민국(농촌진흥청장)

Inventor： 변명옥 ,  정미정 ,  박수철 ,  이성곤 ,  권택륜 ,  조우석 ,  윤혜진 ,  신동진 ,  이진옥 ,  박상렬 ,  김범기 ,  한세연 ,  권혁빈 ,  문석준

IPC: C12N15/29 ,  C12N15/09 ,  C12N15/67

Abstract: PURPOSE: A broad host range disease resistance gene OsRBI1 is provided to promote expression of genes the enhance plant disease-resistance. CONSTITUTION: A plant has enhanced disease-resistance by improving intracellular level of polypeptide with an amino acid sequence of the sequence number 1. A method for producing a plant with enhanced disease-resistance comprises: a step of preparing an expression vector which overexpresses a polypeptide with the amino acid of the sequence number 1; and a step of transforming the plant with the expression vector. The expression vector comprises a promoter and a polynucleotide which is operably linked to the promoter and encodes the polypeptide of the sequence number 1.

Abstract translation: 目的：提供广泛的宿主范围抗病基因OsRBI1，以促进基因的表达，增强植物的抗病性。 构成：植物通过用序列号1的氨基酸序列改善多肽的细胞内水平来增强抗病性。一种具有增强的抗病性的植物的制备方法包括：制备过表达多肽的表达载体的步骤 与序列号1的氨基酸; 和用表达载体转化植物的步骤。 表达载体包含启动子和可操作地连接到启动子并编码序列号1的多肽的多核苷酸。