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公开(公告)号：KR1020130055050A
公开(公告)日：2013-05-28
申请号：KR1020110119572
申请日：2011-11-16
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8273 , A01G22/00 , C12N15/8205 , C12N15/8209 , C12N15/8216
Abstract: PURPOSE: A transgenic plant which is transformed by a freezing injury and drought tolerant gene is provided to improve freezing injury and drought tolerance, and to remarkably improve productivity of Solanaceae. CONSTITUTION: A method for improving freezing injury and drought tolerance in a plant comprises a step of overexpressing a polypeptide with an amino acid sequence of sequence number 2. The polypeptide is overexpressed by transforming a recombinant expression vector containing a promoter and a gene encoding the polypeptide in a plant or by introducing the gene into a plant cell. The gene encoding the polypeptide has a base sequence of sequence number 1. The plant includes potato, tomato, Capsicum annuum, bean, eggplant, or tobacco. A method for producing a plant with freezing injury and drought tolerance comprises a step of transforming the recombinant expression vector to the plant. A transformant with improved freezing injury and drought tolerance is obtained by transforming with the recombinant expression vector.
Abstract translation: 目的：提供通过冷冻损伤和耐旱基因转化的转基因植物，以改善冷冻伤害和耐旱性，并显着提高茄科的生产力。 构成：用于改善植物中的冷冻损伤和耐旱性的方法包括用序列号2的氨基酸序列过多表达多肽的步骤。通过转化含有启动子和编码多肽的基因的重组表达载体，所述多肽被过表达 在植物中或通过将基因导入植物细胞。 编码该多肽的基因具有序列号1的碱基序列。该植物包括马铃薯，番茄，辣椒，豆，茄子或烟草。 用于生产具有冷冻损伤和耐旱性的植物的方法包括将重组表达载体转化至植物的步骤。 通过用重组表达载体转化获得具有改善的冷冻损伤和耐旱性的转化体。
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公开(公告)号：KR1020040050634A
公开(公告)日：2004-06-16
申请号：KR1020020078495
申请日：2002-12-10
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/29
CPC classification number: C12N15/8273
Abstract: PURPOSE: A gene related to dehydration tolerance of potato and dehydration-tolerant plant using the same are provided, thereby amplifying the expression of the gene when the plant is faced with dehydration, so that the plant can grow under dehydration condition. CONSTITUTION: The gene StRD22 related to dehydration tolerance of potato is isolated from potato and has the nucleotide sequence set forth in SEQ ID NO: 1, wherein the gene StRD22 is isolated by the steps of: freezing potato treated at 4 deg. C for 8 hours and untreated potato and pulverizing them; extracting RNA from the pulverized potatoes; isolating poly A+RNA from the extracted total RNA; subjecting the poly A+RNA to RNase H-reverse transcriptase to synthesize first strand cDNA; removing RNA using RNase and synthesizing second strand cDNA using DNA polymerase I; inserting the synthesized cDNA into Uni-zap XR vector; in vitro packaging the cDNA inserted vector; infecting E. coli with the cDNA inserted vector to determine Phage titer and amplify the genes; and sequencing the genes.
Abstract translation: 目的：提供一种与马铃薯和脱水耐受性相关的脱水耐性相关基因，从而在植物面临脱水时扩增基因的表达，使植物在脱水条件下生长。 构成：与马铃薯的脱水耐性相关的基因StRD22与马铃薯分离，具有SEQ ID NO：1所示的核苷酸序列，其中通过以下步骤分离基因StRD22：将4℃处理的马铃薯冷冻。 8小时，未处理的马铃薯并粉碎; 从粉碎的马铃薯中提取RNA; 从提取的总RNA中分离聚A + RNA; 将聚A + RNA转染至RNA酶H逆转录酶合成第一链cDNA; 使用RNA酶除去RNA并使用DNA聚合酶I合成第二链cDNA; 将合成的cDNA插入到Uni-zap XR载体中; 体外包装cDNA插入载体; 用cDNA插入载体感染大肠杆菌以确定噬菌体滴度并扩增基因; 并对基因进行测序。
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3.发明公开느타리 버섯으로부터 분리된 신규한 ＧＰＤ유전자를 형질전환시킨 오스모틱 스트레스 내성 효모 有权由包含从PLEUROTUS SAJOR-CAJU分离的GPD基因的重组载体转化的耐摩擦重组酵母
公开(公告)号：KR1020010011673A
公开(公告)日：2001-02-15
申请号：KR1019990031152
申请日：1999-07-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/81
Abstract: PURPOSE: An osmotic stress resistant yeast transformed by a recombinant vector containing the GPD gene that isolated from Pleurotus sajor-caju is provided for improving the osmotic stress resistance of yeast. CONSTITUTION: The osmotic stress resistant yeast transformed by a recombinant vector containing glycerol-3-phosphate dehydrogenase(GPD) gene as an osmotic stress related gene is obtained by the following steps of: incubating Pleurotus sajor-caju ASI 2070 in an MCM medium containing 20g of peptone, 2g of yeast extract, 2g of K2HPO4, 1.0g of KH2PO4, 0.46g of MgSO47H2O, 0.5g of DW1L at 25 deg. C for 15 days; filtering with mira cloth; separating total RNA and DNA of Pleurotus sajor-caju ASI 2070 by using a Graham method and a Sambrook method, respectively; preparing the pYES2 vector containing the GPD gene by using restriction enzymes; and transforming Saccharomyces cerevisiae INVSC2 with the pYES2 vector.
Abstract translation: 目的：提供通过含有从侧耳属sajor-caju分离的GPD基因的重组载体转化的渗透胁迫酵母，用于改善酵母的渗透胁迫性。 构成：通过含有甘油-3-磷酸脱氢酶（GPD）基因的重组载体转化的渗透压胁迫酵母作为渗透胁迫相关基因，通过以下步骤获得：将Pleurotus sajor-caju ASI 2070在含有20g的MCM培养基中培养 的蛋白胨，2g酵母提取物，2g K 2 HPO 4，1.0g KH 2 PO 4，0.46g MgSO 4·7H 2 O，0.5g DW1L，25℃。 C 15天; 用米拉布过滤; 分别使用Graham方法和Sambrook方法分离了侧耳菇sajor-caju ASI 2070的总RNA和DNA; 通过使用限制酶制备含有GPD基因的pYES2载体; 并用pYES2载体转化酿酒酵母INVSC2。
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公开(公告)号：KR101049874B1
公开(公告)日：2011-07-19
申请号：KR1020090062798
申请日：2009-07-10
Applicant: 대한민국(농촌진흥청장)
Inventor： 김범기 , 변명옥 , 정미정 , 박수철 , 이성곤 , 권택륜 , 조우석 , 윤혜진 , 박상렬 , 이혜은 , 김둘이 , 신동진 , 이정원 , 한세연 , 권혁빈 , 문석준
Abstract: 본 발명은 내염성 또는 내한발성 유전자 StMyb 및 이의 용도에 관한 것으로서 보다 상세하게는 서열번호 1로 표시되는 아미노산 서열을 갖는 StMyb 폴리펩티드의 세포내 수준을 조절하여 식물의 내염성 또는 내한발성을 증진시키는 방법, 상기 방법에 따라 제조된 식물 및 이의 종자에 관한 것이다. 본 발명의 StMyb을 식물체에 과발현하는 경우 식물의 내염성 또는 내한발성을 증진시키는 효과가 있다. 또한 본 발명의 StMyb를 과발현하는 벡터로 식물을 형질전환하는 방법은 내염성 또는 내한발성이 증진된 식물 및 이의 종자를 제조할 수 있게 하여 내염성 또는 내한발성이 필요한 환경에서 재배할 수 있는 식물 품종 개발에 효과적이다.
감자, StMyb, 내염성, 내한발성, 형질전환체Abstract translation: 本发明耐盐性的或冷的发声基因StMyb并且更详细地涉及它们的使用是如何控制StMyb多肽内的水平包含在SEQ ID NO：2所示的氨基酸序列：1，以提高植物的耐盐性的或冷的发声，则 本发明涉及一种通过该方法生产的植物及其种子。 当本发明的StMyb在植物中过表达时，具有提高植物的耐盐性或耐冷性的效果。 还开发可以在转化植物的载体的过表达本发明耐盐性的或冷的话语的StMyb的方法生长的植物品种是促进植物并且使得有可能制造出计数器种子的耐盐性，或需要冷发声应用 这是有效的。
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公开(公告)号：KR100994422B1
公开(公告)日：2010-11-15
申请号：KR1020080047271
申请日：2008-05-21
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 벼 광역기주 병 방어 유전자 OsRBI1 및 이의 용도에 관한 것으로서 보다 상세하게는 서열번호 1로 표시되는 아미노산 서열을 갖는 OsRBI1 폴리펩티드의 세포내 수준을 조절하여 식물의 병-저항성을 증진시키는 방법, 상기 방법에 따라 제조된 식물에 관한 것이다. 본 발명의 OsRBI1은 식물의 병-저항성에 관여하며, NPR1 등과 상호작용하여 여러 하위 유전자의 발현을 촉진한다. 따라서, 본 발명의 OsRBI1의 세포내 수준을 조절하는 경우 식물의 병-저항성을 증진할 수 있으며, 병-저항성이 증진된 식물 및 이의 종자를 얻을 수 있다.
벼, OsRBI1 ,광역기주 병저항성, 형질전환-
Patent Agency Ranking
公开(公告)号：KR100826140B1
公开(公告)日：2008-04-29
申请号：KR1020070039612
申请日：2007-04-24
Applicant: 대한민국(농촌진흥청장) , 권혁빈
Abstract: An StEREBP gene derived from potato is provided to show tolerance against abiotic disaster such as salt and cold-weather damage and biotic disaster such as bacterial disease tolerance and phytophthora blight tolerance, thereby being used in order to prepare a stress tolerant crop with disaster resistance and disease tolerance. A crop disease and disaster tolerant gene derived from potato is described in a sequence listing under SEQ ID : NO. 1. A crop having diseases and disaster tolerance is transformed by the gene. A transgenic Agrobacterium tumefaciens LBA4404 is characterized in that it is transformed by allowing the gene to be controlled by a 35S promoter in a pBI121 vector and is deposited as a deposition no. KACC 95058P.
Abstract translation: 提供了一种来源于马铃薯的StEREBP基因，以表现出对非生物灾害的耐受性，如盐和寒冷天气损害以及细菌病害耐受性和疫病抗性等生物灾害，从而用于制备具有耐灾性的耐胁迫作物， 疾病耐受性。 来自马铃薯的作物病害和耐受性基因在SEQ ID NO：1的序列表中描述。 具有疾病和抗灾能力的作物被基因转化。 转基因根癌土壤杆菌LBA4404的特征在于通过使pBI121载体中的35S启动子控制该基因而进行转化，并将其沉积为沉积物。 KACC 95058P。
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公开(公告)号：KR101341932B1
公开(公告)日：2013-12-16
申请号：KR1020110119572
申请日：2011-11-16
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은동해및 가뭄저항성폴리펩티드의세포내 수준을증가시켜식물의동해및 가뭄저항성을향상시키는방법에관한것으로, 본발명에따라동해및 가뭄에대한저항성유전자로형질전환된식물체는동해및 가뭄저항성품종으로육성가능하다.
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公开(公告)号：KR1020100007772A
公开(公告)日：2010-01-22
申请号：KR1020090062798
申请日：2009-07-10
Applicant: 대한민국(농촌진흥청장)
Inventor： 김범기 , 변명옥 , 정미정 , 박수철 , 이성곤 , 권택륜 , 조우석 , 윤혜진 , 박상렬 , 이혜은 , 김둘이 , 신동진 , 이정원 , 한세연 , 권혁빈 , 문석준
Abstract: PURPOSE: An StMyb gene having salt-resistance or drought-resistance of plant is provided to use in developing plant species. CONSTITUTION: An StMyb(Solanum tuberosum Myb) polypeptide comprises an amino acid of sequence number 1. An StMyb polynucleotide has a gene sequence of sequence number 2. The intracellular increase of StMyb polypeptide is performed by transforming a plant with a vector containing the polynucleotide and a promoter. A method for producing the plant enhancing salt tolerance or drought tolerance comprises: a step of overexpressing StMyb polypeptide having amino acid of sequence number 1; and a step of transforming the plant with the expression vector.
Abstract translation: 目的：提供植物耐盐性或抗旱性的StMyb基因，用于开发植物物种。 构成：StMyb（Solanum tuberosum Myb）多肽包含序列号1的氨基酸.StMyb多核苷酸具有序列号2的基因序列。通过用含有多核苷酸的载体转化植物，进行StMyb多肽的细胞内增加， 一个启动子 用于产生植物增强耐盐性或耐旱性的方法包括：过表达具有序列号1的氨基酸的StMyb多肽的步骤; 和用表达载体转化植物的步骤。
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公开(公告)号：KR100514433B1
公开(公告)日：2005-09-14
申请号：KR1020020078495
申请日：2002-12-10
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/29
Abstract: 본 발명은 감자 한발적응 유전자에 관한 것으로서, 본 발명의 감자 한발 적응 유전자인 StRD22 유전자는 식물이 한발상태에 처할 때 발현이 증폭되는 유전자로서, 이 유전자를 식물형질전환벡터에 실어 담배에 형질전환한 결과 한발후 회복능력이 크게 증진됨이 확인됨으로서 한발내성 작물개발에 활용할 수 있게된다.
이러한 본 발명에서는 저온처리된 감자에서 cDNA유전자은행을 작성하고 reverse northern에 의해 선발된 유전자의 염기서열분석 및 상동성 분석 결과에 의해 StRD22 유전자로 추정되고 그의 발현분석 및 이 유전자를 환경특이 발현 promoter에 결합시켜 담배에 형질전환을 실시한 후 형질전환체의 한발 적응 능력을 검정하였다.-
公开(公告)号：KR1020040050633A
公开(公告)日：2004-06-16
申请号：KR1020020078494
申请日：2002-12-10
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/29
CPC classification number: C12N15/8273
Abstract: PURPOSE: A gene related to freezing tolerance of potato and freezing-tolerant plants using the same are provided, thereby operating transcription factor through the signal transfer process when the plant is placed at low temperature to amplify the expression of other genes, so that the plant can grow at low temperature. CONSTITUTION: The gene StEREBP related to freezing tolerance of potato is isolated from potato and has the nucleotide sequence set forth in SEQ ID NO: 1, wherein the gene StEREBP is isolated by the steps of: freezing potato treated at 4 deg. C for 8 hours and untreated potato and pulverizing them; extracting RNA from the pulverized potatoes; isolating poly A+RNA from the extracted total RNA; subjecting the poly A+RNA to RNase H-reverse transcriptase to synthesize first strand cDNA; removing RNA using RNase and synthesizing second strand cDNA using DNA polymerase I; inserting the synthesized cDNA into Uni-zap XR vector; in vitro packaging the cDNA inserted vector; infecting E. coli with the cDNA inserted vector to determine Phage titer and amplify the genes; and sequencing the genes.
Abstract translation: 目的：提供与使用其相同的马铃薯和耐寒植物的耐寒性相关基因，从而当植物放置在低温下以扩增其他基因的表达时，通过信号转移过程来操作转录因子，从而使植物 可以在低温下生长。 构成：从马铃薯中分离与马铃薯的耐寒性相关的基因StEREBP，并且具有SEQ ID NO：1所示的核苷酸序列，其中通过以下步骤分离基因StEREBP：冷冻马铃薯处理4℃。 8小时，未处理的马铃薯并粉碎; 从粉碎的马铃薯中提取RNA; 从提取的总RNA中分离聚A + RNA; 将聚A + RNA转染至RNA酶H逆转录酶合成第一链cDNA; 使用RNA酶除去RNA并使用DNA聚合酶I合成第二链cDNA; 将合成的cDNA插入到Uni-zap XR载体中; 体外包装cDNA插入载体; 用cDNA插入载体感染大肠杆菌以确定噬菌体滴度并扩增基因; 并对基因进行测序。
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