Abstract:
The present invention relates to an oligonucleotide probe set to specifically diagnose or detect Tobacco necrosis virus (TNV), genechips comprising the oligonucleotide probe set to specifically diagnose and detect the TNV, a method to specifically diagnose or detect the TNV using the oligonucleotide probe set, and a kit comprising the oligonucleotide probe set and a hybridization solution to specifically diagnose or detect the TNV. Since the TNV is specifically detectable using the probe set of the present invention, the probe set is expected to be useful in increasing the production of domestic agricultural products by minimizing the viral diseases of the domestic agricultural products.
Abstract:
The present invention relates to a method for detecting and distinguishing a host-specific strain of a Tomato yellow leaf curl virus (TYLCV) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). More specifically, the present invention relates to a method for detecting and distinguishing Korea, Israel, Spain, USA, Jordan 1, Jordan 2, and Jordan 3 which are seven strains of the Tomato yellow leaf curl virus (TYLCV) from an amplified product, which is amplified using a primer set for PCR for detecting a host-specific strain of a TYLCV, using Dde I, Fau I, or Bss SI which is a restriction enzyme. By means of the method according to the present invention, the infection of the TYLCV and the strain can be effectively confirmed within a short period of time without high-priced technical equipment such as sequence analysis equipment. Accordingly, the spread of the TYLCV is domestically prevented, and the inflow of domestically non-occurred strains can be prevented in a plant quarantine step, so that a huge economical loss can be remarkably reduced.