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公开(公告)号：KR101423395B1
公开(公告)日：2014-07-24
申请号：KR1020120124504
申请日：2012-11-05
Applicant: 성균관대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 토마토황화잎말림바이러스를 검출하기 위한 등온증폭반응용 프라이머 세트, 이를 포함하는 조성물, 및 상기 조성물을 이용한 토마토황화잎말림바이러스 검출방법에 관한 것으로, 보다 구체적으로는 토마토황화잎말림바이러스의 검출을 위한 등온증폭 반응용 4개의 프라이머 세트와 이를 포함하는 프라이머 조성물 및 검출방법에 관한 것이다. 본 발명에 따른 검출방법은 등온증폭법을 이용하여 단시간 내에 전문장비 없이 효과적으로 토마토황화잎말림바이러스를 검출할 수 있다. 또한 고농도의 SYBR Green I을 이용하여 자연광하에서 육안으로 신속하게 진단할 수 있다. 따라서 토마토재배농가에 막대한 피해를 줄 수 있는 토마토 감염성 바이러스를 조기에 검출 가능하게 하여 보다 신속하고 효율적인 토마토 바이러스 진단 시스템을 구축할 수 있을 것으로 기대된다.
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公开(公告)号：KR1020130049764A
公开(公告)日：2013-05-14
申请号：KR1020120124503
申请日：2012-11-05
Applicant: 성균관대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C12Q1/6844 , C12Q1/04 , C12Q2527/101
Abstract: PURPOSE: A primer composition for loop-mediated isothermal amplification reaction for detecting curtovirus, and a use thereof are provided to quickly diagnose the virus using SYBR green I with naked eye, and to early detect the virus. CONSTITUTION: A primer set for loop-mediated isothermal amplification reaction for detecting curtovirus contains sequence numbers 1-4. The cultovirus is selected from the group consisting of beet curly top virus(BCTV), beet severe curly top virus(BSCTV), and beet mild curly top virus(BMCTV). A primer composition for loop-mediated isothermal amplification reaction for detecting curtovirus contains a primer set, and further contains DNA polymerase, dNTPs, and a reaction buffer.
Abstract translation: 目的：用于检测曲霉病毒的环介导等温扩增反应的底漆组合物，并提供其用途，以用肉眼快速诊断病毒，并使用SYBR green I进行早期检测。 构成：用于检测curtovirus的环介导等温扩增反应的引物组包含序列号1-4。 火鸡病毒选自甜菜卷曲病毒（BCTV），甜菜严重卷曲病毒（BSCTV）和甜菜轻度卷曲病毒（BMCTV）组成。 用于检测curtovirus的环介导的等温扩增反应的引物组合含有引物组，并且还含有DNA聚合酶，dNTP和反应缓冲液。
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3.发明公开중합효소 연쇄 반응 및 제한효소 절편길이 다형성을 이용하여 토마토황화잎말림바이러스의 기주특이적 변이주를 검출 및 구별하는 방법 无效使用聚合酶链反应限制片段长度多态性检测和主要特异性菌株分离TOMATO黄叶病毒病毒的方法
公开(公告)号：KR1020140062936A
公开(公告)日：2014-05-27
申请号：KR1020120129258
申请日：2012-11-15
Applicant: 성균관대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: The present invention relates to a method for detecting and distinguishing a host-specific strain of a Tomato yellow leaf curl virus (TYLCV) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). More specifically, the present invention relates to a method for detecting and distinguishing Korea, Israel, Spain, USA, Jordan 1, Jordan 2, and Jordan 3 which are seven strains of the Tomato yellow leaf curl virus (TYLCV) from an amplified product, which is amplified using a primer set for PCR for detecting a host-specific strain of a TYLCV, using Dde I, Fau I, or Bss SI which is a restriction enzyme. By means of the method according to the present invention, the infection of the TYLCV and the strain can be effectively confirmed within a short period of time without high-priced technical equipment such as sequence analysis equipment. Accordingly, the spread of the TYLCV is domestically prevented, and the inflow of domestically non-occurred strains can be prevented in a plant quarantine step, so that a huge economical loss can be remarkably reduced.
Abstract translation: 本发明涉及使用聚合酶链反应限制性片段长度多态性（PCR-RFLP）检测和鉴别番茄黄叶卷曲病毒（TYLCV）的宿主特异性菌株的方法。 更具体地说，本发明涉及一种用于从扩增产物中检测和区分来自番茄黄叶卷曲病毒（TYLCV）的7株的韩国，以色列，西班牙，美国，约旦1号，约旦2号和约旦3号的方法， 其使用用于PCR的引物组进行扩增，用于检测TYLCV的宿主特异性菌株，使用DdeI，FauI或BssI， SI是限制酶。 通过本发明的方法，可以在短时间内有效地确认TYLCV和菌株的感染，而不需要高价位的技术设备如序列分析设备。 因此，TYLCV的扩散在国内被防止，在植物检疫步骤中可以防止国内未发生菌株的流入，从而显着降低了巨大的经济损失。
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公开(公告)号：KR1020130049765A
公开(公告)日：2013-05-14
申请号：KR1020120124504
申请日：2012-11-05
Applicant: 성균관대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C12Q1/6844 , C12Q1/04 , C12Q2527/101
Abstract: PURPOSE: A primer composition for loop-mediated isothermal amplification reaction for detecting tomato yellow leaf curl virus is provided to quickly diagnose the virus using high concentration SYBR Green I under natural light with naked eye. CONSTITUTION: A primer set for loop-mediated isothermal amplification reaction for detecting tomato yellow leaf curl virus has sequence numbers 1-4. The primer set has sequence numbers 11-14. The virus is selected from the group consisting of TYLCV Jordan1 mutant strains, Jordan3 mutant strains, Israel mutant strains, Spain mutant strains, USA mutant strains, and Korea mutant strains. A primer composition for loop-mediated isothermal amplification reaction for detecting the virus contains the primer set, DNA polymerase, dNTPs, and a reaction buffer.
Abstract translation: 目的：提供用于检测番茄黄叶卷曲病毒的环介导等温扩增反应的底漆组合物，用肉眼自然光快速诊断病毒，使用高浓度SYBR Green I。 构成：用于检测番茄黄叶卷曲病毒的环介导等温扩增反应的引物组具有序列号1-4。 引物组序列号为11-14。 病毒选自TYLCV Jordan1突变株，Jordan3突变株，以色列突变菌株，西班牙突变菌株，USA突变菌株和韩国突变菌株。 用于检测病毒的环介导的等温扩增反应的引物组合物含有引物组，DNA聚合酶，dNTP和反应缓冲液。
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5.发明公开중합효소 연쇄 반응 및 제한효소 절편길이 다형성을 이용하여 컬토바이러스 속에 속하는 바이러스 종을 검출 및 구별하는 방법 无效使用聚合酶链反应限制片段长度多态性检测和染色病毒性病毒的方法
公开(公告)号：KR1020140062937A
公开(公告)日：2014-05-27
申请号：KR1020120129263
申请日：2012-11-15
Applicant: 성균관대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: The present invention relates to a method for detecting and distinguishing a beet curly top virus, a beet severe curly top virus, and a beet mild curly top virus which are viruses of the genus curtovirus using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). More specifically, the present invention relates to a method for detecting and distinguishing each of the viruses by confirming pieces after treating Dra I, which is a restriction enzyme, on a byproduct that is amplified using a primer set for PCR that can amplify all of the three viruses. By means of the method according to the present invention, three species of the curtovirus can be effectively diagnosed and distinguished within a short period of time without high-priced technical equipment such as sequence analysis equipment. The species are not reported to have occurred in Korea but the viruses often occur in major agricultural importers including North America. Accordingly, the curtovirus that can severely damage farms is rapidly and accurately detected in a preemptive plant quarantine step so that a huge economical loss can be remarkably reduced.
Abstract translation: 本发明涉及使用聚合酶链反应限制性片段长度多态性（PCR-PCR）检测和鉴别作为曲霉病病毒的甜菜卷曲病毒，甜菜重度卷曲病毒和甜菜轻度卷曲病毒的方法， RFLP）。 更具体地说，本发明涉及一种用于通过将作为限制性内切酶处理DraI之后的片段在使用引物组扩增的副产物上确认片段来检测和区分每种病毒的方法， 可以扩增所有三种病毒的PCR。 通过本发明的方法，可以在短时间内有效地诊断和鉴别三种curtovirus，而不需要高价位的技术设备如序列分析设备。 该物种不报告在韩国发生，但病毒通常发生在包括北美在内的主要农业进口国。 因此，可以在抢先的植物检疫步骤中快速，准确地检测到严重损害农场的curtovirus，从而可以显着降低巨大的经济损失。
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Patent Agency Ranking
公开(公告)号：KR101481245B1
公开(公告)日：2015-01-12
申请号：KR1020120156472
申请日：2012-12-28
Applicant: 성균관대학교산학협력단
Abstract: 본 발명은 스쿼시모자이크바이러스를 검출하기 위한 4개의 등온증폭반응용 프라이머 세트, 이를 포함하는 조성물, 및 상기 조성물을 이용한 스쿼시모자이크바이러스 검출방법에 관한 것이다. 본 발명의 프라이머 세트를 사용하면 등온증폭법을 이용하여 단시간 내에 전문장비 없이 효과적으로 스쿼시모자이크바이러스의 5종의 변이주를 모두 검출할 수 있다. 또한 고농도의 SYBR Green I을 이용하여 자연광하에서 육안으로 신속하게 진단할 수 있다. 따라서 호박, 멜론, 수박 등의 작물의 재배농가에 막대한 피해를 주고 있는 바이러스를 조기에 검출 가능하게 하여 보다 신속하고 효율적인 스쿼시모자이크바이러스 진단 시스템을 구축할 수 있을 것으로 기대된다.
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公开(公告)号：KR1020140086236A
公开(公告)日：2014-07-08
申请号：KR1020120156473
申请日：2012-12-28
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/6844 , C12Q2527/101 , C12Q2563/107 , C12Q2565/125
Abstract: The present invention relates to a set consisting of four primers for an isothermal amplification reaction for detecting a watermelon mosaic virus, a composition comprising the same, and a method for detecting watermelon mosaic virus using the composition. The detecting method according to the present invention uses the isothermal amplification method to effectively detect all seven kinds of mutant strains of watermelon mosaic virus without specialized equipment in a short time. Moreover, the virus can be rapidly detected with the naked eye under natural light by using a high concentration of SYBR Green I. Therefore, the virus incurring huge damage to farms growing crops like pumpkin, melon, and watermelon can be detected early, thus the development of a more rapid and effective watermelon mosaic virus diagnosis system is expected.
Abstract translation: 本发明涉及由用于检测西瓜花叶病毒的等温扩增反应的四个引物，包含该引物的组合物以及使用该组合物检测西瓜花叶病毒的方法。 根据本发明的检测方法使用等温扩增方法在短时间内有效地检测所有七种不具有专门设备的西瓜花叶病毒突变菌株。 此外，通过使用高浓度的SYBR Green I，可以在自然光下用肉眼快速检测病毒。因此，可以及早检测到生长在南瓜，西瓜和西瓜等农作物上的巨大损害，因此， 开发更快速有效的西瓜花叶病毒诊断系统。
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8.发明公开
公开(公告)号：KR1020130044375A
公开(公告)日：2013-05-02
申请号：KR1020110108063
申请日：2011-10-21
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/6858 , C12N15/11
Abstract: PURPOSE: A primer set for detecting begomovirus beta satellite DNA and a method for detecting begomovirus beta satellite DNA is provided to have different DNA host plants, capable of detecting a respective primer set, thereby specifically detecting diagnosis of some begomovirus. CONSTITUTION: A primer set for detecting begomovirus beta satellite DNA is selected from a primer set of sequence number 1 and sequence number 2, a primer set of sequence number 3 and sequence number 4, a primer set of sequence number 5 and sequence number 6, and a primer set of sequence number 7 and sequence number 8. The primer set of sequence number 1 and sequence number 2 is for generally detecting begomovirus beta satellite DNA. The primer set of sequence number 3 and sequence number 4 is for detecting HYVMV(Honeysuckle yellow vein mosaic virus). The primer set of sequence number 5 and sequence number 6 is for detecting HYVV(Honeysuckle yellow vein virus). A method for detecting begomovirus beta satellite DNA comprises a step of separating total DNA from a plant sample; a step of amplifying a target sequence by using a primer set; and a step of detecting the amplified product.
Abstract translation: 目的：提供一种用于检测绒毛病毒β卫星DNA的引物组和一种检测酵母病毒β卫星DNA的方法，以具有不同的DNA宿主植物，能够检测各自的引物组，从而特异性检测某些枯叶病毒的诊断。 构成：从序列号1和序列号2的引物组，序列号3和序列号4的引物组，序列号5和序列号6的引物组中选择用于检测蛋白病毒β卫星DNA的引物组， 序列号为7和序列号8的引物组。序列号1和序列号2的引物组通常用于检测艾滋病毒β卫星DNA。 序列号3和序列号4的引物组用于检测HYVMV（金银花黄色静脉花叶病毒）。 序列号5和序列号6的引物组用于检测HYVV（金银花黄色静脉病毒）。 用于检测霉菌病毒β卫星DNA的方法包括从植物样品中分离总DNA的步骤; 通过使用引物组扩增靶序列的步骤; 以及检测扩增产物的步骤。
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