Abstract:
본 발명은 티로시나아제를 이용한 카테콜형 구조 물질의 생합성 반응에 관한 기술로, 티로시나아제의 이차 산화 반응을 효과적으로 억제하여, 모노페놀형 구조 물질의 일차 수산화 반응만을 선택적으로 촉매화하는 기술이다. 본 발명은 이를 이용한 다양한 기능성 카테콜형 물질들을 높은 생산성과 수율로 생산하는 기술을 제공한다.
Abstract:
PURPOSE: A method for preparing ginsenoside using a novel oxidoreductase or novel Rhizobium sp. GIN611 is provided to ensure produce various glycons. CONSTITUTION: A novel Rhizobium sp. GIN611(KCTC 11708P) is isolated from soil. A method for glycolysis of a natural product comprises a step of decomposing glucose of ginsenoside. A substrate is ginsenoside compound K and the product is ginsenoside PPD(S). An oxidoreductase contains an amino acid sequence of sequence number 3. A DNA encoding the oxidoreductase contains a sequence of sequence number 2. The oxidoreductase is derived from Agrobacterium sp., Sphingobacterium sp. or Stenotrophomonase sp.
Abstract:
PURPOSE: A bead-based microchip with a temperature control function is provided to shorten chemical reaction time and to simplify test procedure. CONSTITUTION: A bead-based microchip with a temperature control function comprises: a microchip body(140) with a first reaction chamber(142) and second reaction chamber(144) which are connected through a fluid channel; a bead filled in the reaction chamber in which a ligand is fixed on the surface; a heater(150) which is attached on the lower side of the microchip body; and a cover(130) which is attached at the upper side of the microchip body and has a sample inlet and enzyme inlet.