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公开(公告)号：KR1020080054955A
公开(公告)日：2008-06-19
申请号：KR1020060127701
申请日：2006-12-14
Applicant: 고려대학교 산학협력단
Abstract: A polydiacetylene sensor chip comprising protein is provided to detect rapidly protein-protein or protein-cell reaction without labeling of the subject to be analyzed, and reduce the amount of polydiacetylene and subject to be analyzed. A polydiacetylene sensor chip comprises a substrate, a vesicle bound to the substrate and having polymerized diacetylene represented by the formula(1): A-(L1)d-(CH2)e-C=C-C=C-(CH2)f-(L2)g-B, and a probe protein bound to the vesicle, wherein a sum d and g is 0, 1 or 2; a sum of e and f is an integer from 2 to 50; e and f are each independently an integer of 1 or more; A and B are each independently methyl group, amine group, carboxyl group, hydroxyl group, maleimide group, biotin group, hydroxysuccinimide group, benzoic acid group or ester group; and L1 and L2 are each independently alkyl group having carbon number of 2 or more, at least one ethylene oxide group, amine group, amide group, ester group or carbonyl group.
Abstract translation: 提供了包含蛋白质的聚二乙炔传感器芯片，用于快速检测蛋白质 - 蛋白质或蛋白质 - 细胞反应，而不需要分析对象的标记，并减少聚二乙炔的量并进行分析。 聚二乙炔传感器芯片包括基底，与基底结合并具有由式（1）表示的聚合的二乙炔的囊泡：A-（L1）d-（CH2）eC = CC = C-（CH2）f-（L2） gB和与小泡结合的探针蛋白，其中和d和g为0,1或2; e和f之和为2至50的整数; e和f各自独立地为1或更大的整数; A和B各自独立地为甲基，胺基，羧基，羟基，马来酰亚胺基，生物素基，羟基琥珀酰亚胺基，苯甲酸基或酯基; 且L1和L2各自独立地为碳数为2以上的烷基，至少一个环氧乙烷基，胺基，酰胺基，酯基或羰基。
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公开(公告)号：KR1020070115118A
公开(公告)日：2007-12-05
申请号：KR1020060049045
申请日：2006-05-30
Applicant: 한양대학교 산학협력단 , 고려대학교 산학협력단
Abstract: A polydiacetylene sensor chip is provided to emit fluorescence by sensing a change in the environment, and thus be applicable to manufacture of various biochips. A method for manufacturing a polydiacetylene array sensor chip includes the steps of: (i) dissolving a diacetylene monomer having a structure represented by the following formula: A-(L1)d-(CH2)e-C=C-C=C-(CH2)f-(L2)g-B in an organic solvent to mix the solution with water or mixing the diacetylene monomer with water directly, and then treating the mixture with ultrasonic waves to obtain an aqueous diacetylene dispersion; and (ii) exposing the aqueous diacetylene dispersion to ultraviolet rays to obtain polydiacetylene, and then fixing the polydiacetylene to a solid substrate using a microarray manufacture apparatus, or fixing the aqueous diacetylene dispersion to the solid substrate using a microarray manufacture apparatus, and then exposing the resultant to ultraviolet rays under the same condition to obtain polydiacetylene.
Abstract translation: 提供聚二乙炔传感器芯片以通过感测环境变化来发射荧光，因此可用于制造各种生物芯片。 制备聚二乙炔阵列传感器芯片的方法包括以下步骤：（i）溶解具有由下式表示的结构的联乙炔单体：A-（L1）d-（CH2）eC = CC = C-（CH2）f - （L2）gB在有机溶剂中混合溶液与水或直接混合二乙炔单体，然后用超声波处理混合物，得到二乙炔水分散体; 和（ii）将水性双乙炔分散体暴露于紫外线以获得聚二乙炔，然后使用微阵列制造装置将聚二乙炔固定在固体基质上，或者使用微阵列制造装置将该二乙炔分散体固定在固体基质上，然后暴露 在相同条件下产生紫外线，得到聚二乙炔。
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公开(公告)号：KR101829677B1
公开(公告)日：2018-02-19
申请号：KR1020160051761
申请日：2016-04-27
Applicant: 고려대학교 산학협력단
Abstract: 본발명은표적물질-특이적핵산이고정된유기반도체를포함하는, 형광복합체, 이의제조방법및 이를이용한표적물질의검출방법에관한것이다. 본발명에따른형광복합체는표적물질-특이적핵산이고정된유기반도체와표적물질의특이적반응을이용함으로써, 표적물질에대한민감도및 선택도가우수하여표적물질의분석이용이하고, 상기형광복합체를이용하여표적물질을검출하는경우, 단순하고경제적인방법으로우수한표적물질검출효과를나타낸다.
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公开(公告)号：KR1020170122587A
公开(公告)日：2017-11-06
申请号：KR1020160051761
申请日：2016-04-27
Applicant: 고려대학교 산학협력단
CPC classification number: C09K11/06 , C12Q1/6816 , C12Q2563/107 , H01L51/50
Abstract: 본발명은표적물질-특이적핵산이고정된유기반도체를포함하는, 형광복합체, 이의제조방법및 이를이용한표적물질의검출방법에관한것이다. 본발명에따른형광복합체는표적물질-특이적핵산이고정된유기반도체와표적물질의특이적반응을이용함으로써, 표적물질에대한민감도및 선택도가우수하여표적물질의분석이용이하고, 상기형광복합체를이용하여표적물질을검출하는경우, 단순하고경제적인방법으로우수한표적물질검출효과를나타낸다.
Abstract translation: 本发明的目标材料 - 涉及用于检测特定核酸，其包含固定的有机半导体，荧光复合物，及其制备方法和使用相同的靶材料的方法。 根据本发明的目标材料的荧光复合物 - 通过使用特定的有机半导体和目标材料核酸的特异性反应是固定的，优异的灵敏度和选择性靶材料度，并且用于目标材料的分析，荧光复 使用检测目标物质，简单，当与表示在目标材料探测经济的方式的优异效果。
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Patent Agency Ranking
公开(公告)号：KR1020120135888A
公开(公告)日：2012-12-17
申请号：KR1020120060864
申请日：2012-06-07
Applicant: 고려대학교 산학협력단
CPC classification number: C07C209/00 , B01J19/10 , B01J2219/0877 , B82Y30/00 , B82Y40/00 , C07C51/412 , Y10T428/2982
Abstract: PURPOSE: Porous diacetylene particles contain larger surface area than the existing type of polydiacetylene sensor, thereby improving the detection limit and detecting time of chemicals and biological molecules harmful to a human body. CONSTITUTION: Porous diacetylene particles are obtained by ion bonding the dicarbocylic acid or diamine monomer containing diacetylene expressed by the chemical equation 1 mentioned below: G-(A)x-C≡C-C≡C-(B)y-G with the diamine or dicarboxylic acid monomer expressed by the chemical equation 2 mentioned below: Q-(D)m-Z-(E)n-Q. For the chemical equation 1 mentioned above, A and B are selected among the substituted or nonsubstituted alkyl, ethylene oxide, amide, and ester, G is selected from COOH or NH2, and x and y are integers from 1 to 20. For the chemical equation 2 mentioned above, Z is selected among the substituted or nonsubstituted alkyl, benzene, cycloalkyl, pyridine, pyrimidine, and naphthalene, D and E are selected among the substituted or nonsubstituted alkyl, benzene, cycloalkyl, pyridine, pyrimidine, and naphthalene, Q is selected from COOH or NH2, and m and n are integers from 0 to 20.
Abstract translation: 目的：多孔乙炔颗粒比现有类型的聚二乙炔传感器具有更大的表面积，从而提高对人体有害的化学物质和生物分子的检测限和检测时间。 构成：通过将下述化学式1表示的含有二乙炔的二羧酸或二胺单体与二胺或二羧酸单体进行离子键合获得多孔乙炔颗粒：G-（A）xC≡CC≡C-（B）yG 通过下述化学方程式2：Q-（D）mZ-（E）nQ。 对于上述化学方程式1，A和B选自取代或未取代的烷基，环氧乙烷，酰胺和酯，G选自COOH或NH 2，x和y为1至20的整数。对于化学 取代或非取代的烷基，苯，环烷基，吡啶，嘧啶和萘中的Z选自取代或未取代的烷基，苯，环烷基，吡啶，嘧啶和萘中的D，E，Q 选自COOH或NH 2，m和n为0〜20的整数。
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公开(公告)号：KR100953182B1
公开(公告)日：2010-04-15
申请号：KR1020060049045
申请日：2006-05-30
Applicant: 한양대학교 산학협력단 , 고려대학교 산학협력단
Abstract: 본 발명은 환경의 변화를 인식하여 형광을 발색시킬 수 있는 다이아세틸렌의 중합체가 고체기판위에 고정된 폴리다이아세틸렌 센서칩 및 그의 제조방법을 제공한다. 본 발명의 폴리다이아세틸렌 센서칩의 제조방법은 (ⅰ) 다이아세틸렌 단량체를 유기용매에 용해시키고, 이를 수용액에 혼합한 다음, 초음파 처리하여 분산된 다이아세틸렌 수용액을 수득하는 공정; 및, (ⅱ) 전기 분산된 다이아세틸렌 수용액을 자외선에 노광하여 폴리다이아세틸렌을 수득하고, 이를 마이크로어레이 제조장치에 적용하여 고체기판 위에 고정시키나, 또는 전기 분산된 다이아세틸렌 용액을 마이크로어레이 제조장치에 적용하여 고체기판 위에 고정시키고, 동일한 조건으로 자외선에 노광하여 폴리다이아세틸렌을 수득하는 공정을 포함한다. 본 발명의 폴리다이아세틸렌 센서칩은 온도, 탄수화물, 단백질 및 DNA인식과 같은 환경의 변화를 감지할 수 있으므로, 다양한 바이오 칩의 제작에 널리 활용될 수 있을 것이다.
다이아세틸렌 단량체, 폴리다이아세틸렌, 센서칩-
公开(公告)号：KR1020100078268A
公开(公告)日：2010-07-08
申请号：KR1020080136478
申请日：2008-12-30
Applicant: 주식회사농심 , 고려대학교 산학협력단
CPC classification number: G01N33/573 , C12Q1/6834 , C12Q1/6888 , G01N33/5308 , G01N33/535
Abstract: PURPOSE: A detection method of 2,4,5-trichlorophenoxyacetic acid is provided to effectively detect agricultural pesticides remaining in agricultural products and processed food by quickly analyze a large number of samples and to save analyzing time and analyzing costs. CONSTITUTION: A detection method of 2,4,5-trichlorophenoxyacetic acid comprises the following steps: combining an antibody, produced from a sample including 2,4,5-trichlorophenoxyacetic acid and a complex in which a hapten compound of the 2,4,5-trichlorophenoxyacetic acid and protein are combined through an amide bond; and detecting the bond of the antibody to the 2,4,5-trichlorophenoxyacetic acid which is fixed to the solid supporter using a secondary antibody marked as an enzyme which is specifically combined with the antibody.
Abstract translation: 目的：提供2,4,5-三氯苯氧乙酸的检测方法，通过快速分析大量样品，节省分析时间和分析成本，有效检测农产品和加工食品中残留的农药残留。 构成：2,4,5-三氯苯氧基乙酸的检测方法包括以下步骤：将由包含2,4,5-三氯苯氧基乙酸的样品和其中2,4,6-三氯苯氧基乙酸的半抗原化合物， 5-三氯苯氧基乙酸和蛋白质通过酰胺键合并; 并使用标记为与抗体特异性结合的酶的二抗检测抗体与固定于固体支持物上的2,4,5-三氯苯氧基乙酸的键。
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公开(公告)号：KR1020100058204A
公开(公告)日：2010-06-03
申请号：KR1020080116938
申请日：2008-11-24
Applicant: 고려대학교 산학협력단
Inventor： 안동준
CPC classification number: H05B3/12 , H05B3/0033 , H05B3/146 , H05B3/56 , H05B6/00
Abstract: PURPOSE: An actively controllable heat distributor is provided to control heat emission of a nano wire by forming a simuli-responsive substance near the nano wire. CONSTITUTION: A heat distributor(100) includes a nano wire(110) and a simuli-responsive material(120). The nano wire is adjacent to a magnetic pole reactive material. The nano wire is a metal nano wire, a semiconductor nano wire, an insulation nano wire, or an inorganic nano wire. The molecular orientation of the magnetic pole reactive material is varied by electric field, magnetic field, light, heat, or pH. The magnetic reactive material is high molecular polyelectrolyte or light-reactive material.
Abstract translation: 目的：提供一种主动控制的热分布器，通过在纳米线附近形成模拟响应物质来控制纳米线的散热。 构成：热分配器（100）包括纳米线（110）和模拟响应材料（120）。 纳米线与磁极反应性材料相邻。 纳米线是金属纳米线，半导体纳米线，绝缘纳米线或无机纳米线。 磁极反应性材料的分子取向由电场，磁场，光，热或pH变化。 磁性反应性材料是高分子聚电解质或光反应性材料。
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公开(公告)号：KR1020090101649A
公开(公告)日：2009-09-29
申请号：KR1020080026922
申请日：2008-03-24
Applicant: 고려대학교 산학협력단
CPC classification number: G01N25/20 , B82Y35/00 , G01N1/28 , G01N21/6402
Abstract: PURPOSE: A method for measuring the thermal characteristic of a micro/nano element by using polydiacetylene supermolecules is provided to remove the necessity of correcting the emissivity coefficient of an inspected object and an infrared camera. CONSTITUTION: A method for measuring the thermal characteristic of a micro/nano element by using polydiacetylene supermolecules comprises the following steps of: making a dispersion solution by mixing the polydiacetylene supermolecules with high molecular solution(S2); coating the dispersion solution on an inspected object(S3); exposing the coated dispersion solution to make the supermolecules into macromolecules(S4); and analyzing the temperature change according to the time by using the phenomenon that the supermolecules self-fluoresces due to the increase of temperature which is generated as a voltage is applied to the inspected object(S5).
Abstract translation: 目的：提供一种通过使用聚二乙炔超分子测量微/纳元件的热特性的方法，以消除校正被检物和红外摄像机的辐射系数的必要性。 构成：使用聚二乙炔超分子测量微/纳元件的热特性的方法包括以下步骤：通过将聚二乙炔超分子与高分子溶液（S2）混合来制备分散溶液; 将分散溶液涂布在检查对象上（S3）; 暴露涂覆的分散液使超分子成为大分子（S4）; 并且通过使用超分子由于随着电压被施加到检查对象而产生的温度的升高而自发荧光的现象来分析温度变化（S5）。
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