公开(公告)号：KR1020130049756A

公开(公告)日：2013-05-14

申请号：KR1020120123844

申请日：2012-11-02

Applicant: 성균관대학교산학협력단

Inventor： 이석찬 ,  박정안 ,  정유철 ,  길의준 ,  김재덕 ,  최홍수 ,  이경열

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

CPC classification number: C12Q1/6844 ,  C12Q1/04 ,  C12Q2527/101

Abstract: PURPOSE: A primer composition for loop-mediated isothermal amplification reaction for beta-satellite DNA of Honeysuckle yellow vein mosaic virus is provided to enable quick diagnosis using high concentration SYBR green I with naked eye. CONSTITUTION: A primer set for loop-mediated isothermal amplification reaction for beta-satellite DNA of Honeysuckle yellow vein mosaic virus has sequence numbers 1-4. The virus is selected from the group consisting of Korea mutant strains, NC005052 mutant strains, and AJ543430 mutant strains. A primer composition for loop-mediated isothermal amplification reaction for beta-satellite DNA of Honeysuckle yellow vein mosaic virus contains the primer set. The composition further contains DNA polymerase, dNTPs, and a reaction buffer.

Abstract translation: 目的：提供用于金银花黄色静脉花叶病毒的β卫星DNA的环介导等温扩增反应的底漆组合物，以便用肉眼可以快速诊断高浓度SYBR绿I。 构成：金银花黄色静脉花叶病毒的β卫星DNA的环介导等温扩增反应的引物组序列号为1-4。 该病毒选自韩国突变株，NC005052突变菌株和AJ543430突变菌株。 金银花黄色静脉花叶病毒的β卫星DNA的环介导等温扩增反应的引物组合含有引物组。 该组合物还含有DNA聚合酶，dNTP和反应缓冲液。