公开(公告)号：KR1020100002586A

公开(公告)日：2010-01-07

申请号：KR1020080062532

申请日：2008-06-30

Applicant: 충남대학교산학협력단 ,  연세대학교 산학협력단

Inventor： 조상래 ,  신성재 ,  김화중 ,  신아름 ,  이승섭

IPC: C12N15/12 ,  C12N15/09 ,  C12Q1/68

CPC classification number: C12Q1/6883

Abstract: PURPOSE: A novel biomarker of serological diagnosis of Crohn's disease is provided to analyze serological relation with M. paratuberculosis and specifically react to serum from Crohn's disease patient. CONSTITUTION: An antigen for diagnosing Crohn's disease is derived from Mycobacterium avium subsp. Paratuberculosis isolated from human and specifically reacts with serum of Crohn's disease patient. A method for isolating the antigen for diagnosing Crohn's disease comprises: a step of detecting protein which specifically reacts with IgG of Crohn's disease patient using pooling serum(n=10) with Mycobacterium Paratuberculosis secretion antigen, and cell extract antigen from human; a step of identifying protein which specifically reacts to the Crohn's disease patient through LC-ESI-MS method; a step of analyzing protein through matching score and bioinformatic analysis; and a step of an identifying protein which specifically reacts to serum of Crohn's disease patient through LC-ESI-MS analysis.

Abstract translation: 目的：提供克罗恩病血清学诊断的新型生物标志物，以分析与副结核分枝杆菌的血清学关系，并与克罗恩病患者的血清特异性反应。 构成：用于诊断克罗恩病的抗原来自鸟分枝杆菌（Mycobacterium avium subsp。）。 从人体分离的特异性结核分枝杆菌与克罗恩病患者的血清特异性反应。 用于分离用于诊断克罗恩病的抗原的方法包括：使用与分枝杆菌分泌抗原的合并血清（n = 10）和来自人的细胞提取物抗原来检测与克罗恩病患者的IgG特异性反应的蛋白质的步骤; 通过LC-ESI-MS方法鉴定与克罗恩病患者特异性反应的蛋白质的步骤; 通过匹配得分和生物信息学分析分析蛋白质的一个步骤; 以及通过LC-ESI-MS分析与克罗恩病患者血清特异性反应的鉴定蛋白质的步骤。

公开(公告)号：KR101893947B1

公开(公告)日：2018-08-31

申请号：KR1020160070510

申请日：2016-06-07

Applicant: 충남대학교산학협력단

Inventor： 김화중 ,  최한규 ,  최승아 ,  백용우

IPC: C12N5/0786 ,  C07K14/35 ,  A61K38/16

Abstract: 본발명은결핵균의 Rv2882c 단백질을유효성분으로포함하는대식세포의활성화조성물에관한것이다. 또한본 발명은결핵균의 Rv2882c 단백질을비활성화된대식세포에처리하여대식세포를활성화시키는방법에관한것이다. 또한상기의대식세포의활성화조성물을포함하는면역증강용조성물에관한것이다.

公开(公告)号：KR101749165B1

公开(公告)日：2017-06-23

申请号：KR1020160070517

申请日：2016-06-07

Applicant: 충남대학교산학협력단 ,  연세대학교 산학협력단

Inventor： 김화중 ,  최한규 ,  신성재

IPC: C12N5/0784 ,  C07K14/35 ,  A61K38/16

CPC classification number: A61K38/16 ,  C07K14/35 ,  C12N5/0639 ,  A61K38/164 ,  C07K2319/00 ,  C12N2501/998

Abstract: 본발명은수지상세포성숙화유도조성물및 수지상세포를성숙시키는방법에관한것이다. 더욱구체적으로는결핵균(M. tuberculosis) 유래의 Rv2299c 또는 Rv2299c 와 ESAT-6를융합한단백질을유효성분으로포함하는수지상세포성숙화유도조성물및 이를이용하여미성숙수지상세포를수지상세포로분화시키는방법에관한것이다. 본발명의방법에의하여수지상세포에의한신체의면역반응을증강시킬수 있다.

Abstract translation: 本发明涉及树突细胞成熟诱导组合物和用于成熟树突细胞的方法。 更具体地说，它使用它们，包括ESAT-6的融合蛋白作为活性成分的树突囚禁涉及euroneun结核分枝杆菌（结核分枝杆菌），或Rv2299c和的树突状细胞成熟的起源和来源的组合物的未成熟树突状细胞的Rv2299c分化的方法 。 通过本发明的方法可以增强树突细胞对身体的免疫反应。

公开(公告)号：KR101290230B1

公开(公告)日：2013-07-30

申请号：KR1020110010165

申请日：2011-02-01

Applicant: 충남대학교산학협력단

Inventor： 신성재 ,  정인덕 ,  박영민 ,  김화중

IPC: A61K39/39 ,  A61K38/10 ,  A61P35/00

Abstract: 본 발명은 수지상 세포 암 치료제에 관한 것이다. 더욱 구체적으로는 결핵균의 Rv0652를 아쥬반트로 이용한 수지상 세포 암 치료제에 관한 것이다. 본 발명의 결핵균의 Rv0652를 아쥬반트로 이용한 수지상 세포 암 치료제는 종양의 성장을 효과적으로 억제시킨다.

公开(公告)号：KR1020130039061A

公开(公告)日：2013-04-19

申请号：KR1020110103498

申请日：2011-10-11

Applicant: 충남대학교산학협력단

Inventor： 신성재 ,  최한규 ,  변의홍 ,  김우식 ,  김종석 ,  김화중

IPC: C12N5/0784 ,  C12N1/38 ,  C07K14/195

Abstract: PURPOSE: A method for maturing dendritic cells is provided to promote differentiation of immature dendritic cells using Mycobacterium tuberculosis Rv2299c, to effectively mature the dendriric cells, and to effectively enhance immune response. CONSTITUTION: A composition for inducing maturation of dendritic cells contains 1-5 microgram/ml of Rv2299c protein derived from Mycobacterium tuberculosis as an active ingredient. A method for inducing maturation of immature dendritic cells comprises: a step of treating Rv2299c to the immature dendritic cells; and a step of culturing the treated dendritic cells for 12-36 hours. The maturation of the immature dendritic cells indicates enhancement of TNF-alpha, IL-12p70, IL-6, and IL-1beta.

Abstract translation: 目的：提供成熟树突状细胞的方法，以促进使用结核分枝杆菌Rv2299c的未成熟树突状细胞的分化，以有效地成熟树突状细胞，并有效增强免疫应答。 构成：用于诱导树突细胞成熟的组合物含有1-5微克/ ml来自结核分枝杆菌的Rv2299c蛋白作为活性成分。 用于诱导未成熟树突状细胞成熟的方法包括：将Rv2299c处理至未成熟树突状细胞的步骤; 以及培养经处理的树突状细胞12-36小时的步骤。 未成熟树突细胞的成熟表明TNF-α，IL-12p70，IL-6和IL-1beta的增强。

公开(公告)号：KR1020130037884A

公开(公告)日：2013-04-17

申请号：KR1020110102394

申请日：2011-10-07

Applicant: 충남대학교산학협력단

Inventor： 신성재 ,  변의홍 ,  김우식 ,  김종석 ,  김화중

IPC: C12N5/0784 ,  C12N1/38 ,  C07K14/35

Abstract: PURPOSE: A method for maturing dendritic cells using Mycobacterium paratuberculosis MAP1948 is provided to promote the differentiation of immature dendritic cells and to effectively mature the dendritic cells. CONSTITUTION: A method for maturing dendritic cells using Mycobacterium paratuberculosis MAP1948 comprises: a step of treating immature resin cells with MAP1948; and a step of culturing the cell for 12-36 hours. The immature resin cells are matured through the increase of IL-6, IL-1beta, and IFN-gamma generation.

Abstract translation: 目的：提供使用分枝杆菌MAP1948成熟树突状细胞的方法，以促进未成熟树突状细胞的分化并有效地成熟树突状细胞。 构成：使用分枝杆菌MAP1948成熟树突状细胞的方法包括：用MAP1948处理未成熟树脂细胞的步骤; 以及培养细胞12-36小时的步骤。 未成熟树脂细胞通过增加IL-6，IL-1β和IFN-γ产生而成熟。

公开(公告)号：KR101082816B1

公开(公告)日：2011-11-11

申请号：KR1020110095102

申请日：2011-09-21

Applicant: 충남대학교산학협력단

Inventor： 신성재 ,  김화중 ,  신아름 ,  오재희

IPC: G01N33/569 ,  G01N33/68

Abstract: 본발명은비결핵항산균중 하나인감염진단용조성물및진단용키트,진단에대한정보를제공하는방법에관한것이다. 보다구체적으로본 발명은수퍼옥사이드디스뮤타제(superoxide dismutase) 또는카탈라아제(catalase)를포함하는진단용조성물, 키트, 진단에대한정보를제공하는방법에관한것이며, 항산균감염증중에서특히고병원성인만을특이적으로진단할수 있다.

公开(公告)号：KR101052350B1

公开(公告)日：2011-07-27

申请号：KR1020100009747

申请日：2010-02-02

Applicant: 충남대학교산학협력단 ,  부산대학교 산학협력단

Inventor： 박영민 ,  정인덕 ,  신성재 ,  김화중

IPC: C07K14/35 ,  C12N5/0784

Abstract: PURPOSE: A method for maturing dendritic cells using heparin binding hemagglutin(HBHA) is provided to effectively activate immune response. CONSTITUTION: A method for maturing dendritic cells comprises a step of treating 0.5-1.0 ug/ml of HBHA(heparin-binding hemagglutin) to immature dendritic cells and a step of culturing for 24 hours. The HBHA is a recombinant HBHA(rMS-HBHA) expressed in M. smegmatis.

Abstract translation: 目的：提供使用肝素结合血凝素（HBHA）成熟树突状细胞的方法，以有效激活免疫应答。 构成：成熟树突细胞的方法包括将0.5-1.0ug / ml的HBHA（肝素结合血凝素）处理成未成熟树突状细胞的步骤和培养24小时的步骤。 HBHA是在耻垢分枝杆菌中表达的重组HBHA（rMS-HBHA）。

公开(公告)号：KR1020110020881A

公开(公告)日：2011-03-03

申请号：KR1020110003716

申请日：2011-01-13

Applicant: 충남대학교산학협력단 ,  연세대학교 산학협력단

Inventor： 조상래 ,  신성재 ,  김화중 ,  신아름 ,  이승섭

IPC: G01N33/53 ,  G01N33/68 ,  C12Q1/68 ,  C12N15/12

CPC classification number: G01N33/6893 ,  C12Q1/6883 ,  C12R1/325 ,  G01N33/5302

Abstract: PURPOSE: A specific protein for serological diagnosis for Crohn's disease is provided to specifically response to a serum from a patient with Crohn's disease. CONSTITUTION: A marker for diagnosing Crohn's disease contains TrpC(Indole-3-glycerolphosphate synthase) which is an antigen specifically responding to serum of Crohn's disease and is derived from human Mycobacterium avium subsp. Paratuberculosis. The marker also contains FbpC1(Fibronectin binding protein C1), FadB1(Fatty oxidation protein B1), LprG(Probable conserve lipoprotein LprG), Gap(Glyceraldehyde 3-phosphate dehydrogenase), Tpi(Triosephosphate isomerase), Tal(Transaldorase), aconitate hydratase, ArgJ(Arginine biosynthesis bifunctional protein), FbpB(Fibronectin binding protein B), HisG(Histidine synthesis protein G), Wag31(Conserved hypothetical protein), Mdh(Malate dehydrogenase), LppZ(Putative lipoprotein), or superoxide dismutase antigen. A composition or kit for diagnosing Crohn's disease contains the marker.

Abstract translation: 目的：提供克罗恩病血清学诊断的特异性蛋白质，以特异性应答克罗恩病患者的血清。 构成：用于诊断克罗恩病的标记物含有TrpC（吲哚-3-磷酸甘油合成酶），其是特异性响应于克罗恩病血清的抗原，来自人类鸟分枝杆菌亚种。 副结核病。 该标记还含有FbpC1（纤连蛋白结合蛋白C1），FadB1（脂肪氧化蛋白B1），LprG（可能保存的脂蛋白LprG），Gap（Glyceraldehyde 3-phosphate dehydrogenase），Tpi（Triosephosphate isomerase），Tal（Transaldorase），itate酸水合酶 ，ArgJ（精氨酸生物合成双功能蛋白），FbpB（纤连蛋白结合蛋白B），HisG（组氨酸合成蛋白G），Wag31（保守假想蛋白），Mdh（苹果酸脱氢酶），LppZ（推定脂蛋白）或超氧化物歧化酶抗原。 用于诊断克罗恩病的组合物或试剂盒含有标记物。

公开(公告)号：KR1020100115927A

公开(公告)日：2010-10-29

申请号：KR1020090034587

申请日：2009-04-21

Applicant: 충남대학교산학협력단

Inventor： 신성재 ,  김화중 ,  신아름 ,  오재희

IPC: G01N33/569 ,  C07K14/005 ,  G01N33/68

Abstract: PURPOSE: A method for screening an antigen for diganosing nonturberculous acid-fast bacteria M. abscessus infection is provided. CONSTITUTION: A method for screening an antigen for diagnosing M. abscessus infection comprises: a step of infecting M. abscessus with epithelial cells or large phagocytes or infecting to epithelial cells and then large phagocytes; a step of confirming specifically expressed M. abscessus protein; a step of reacting the protein and serum from a patient with pulmonary diseases caused by M. abscessus infection; and a step of selecting and identifying the protein.

Abstract translation: 目的：提供一种用于筛选非糖尿病抗性细菌脓肿感染的抗原的方法。 构成：用于筛选用于诊断脓肿脓肿感染的抗原的方法包括：用上皮细胞或大吞噬细胞感染脓肿的感染步骤或感染上皮细胞，然后感染大吞噬细胞的步骤; 确定特异性表达的脓肿蛋白的步骤; 使来自患者的蛋白质和血清与由脓肿脓肿感染引起的肺部疾病反应的步骤; 以及选择和鉴定蛋白质的步骤。