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公开(公告)号：KR1020110039093A
公开(公告)日：2011-04-15
申请号：KR1020090096373
申请日：2009-10-09
Applicant: 한국과학기술연구원 , 한양대학교 산학협력단
CPC classification number: C12Q1/37 , G01N33/533 , G01N33/54306 , G01N2800/102
Abstract: PURPOSE: An in vitro kit for quantitative measurement of protease activity and a method for manufacturing the same are provided to specifically emit strong fluorescence. CONSTITUTION: An in vitro kit for quantitative measurement of protease activity comprises a complex in which fluorescence and quencher are combined and a peptide substrate is conjugated to a polymer. The complex has structural formula 1. The quencher is a blackhole quencher, blackberry quencher, or derivative thereof. A method for manufacturing the kit comprises: a first step of synthesizing a specific peptide substrate to a specific protease and reacting with near infrared ray fluorescence; a second step of binding the peptide substrate with quencher; a third step of binding polymers to fluorescence-peptide substrate-quencher; and a fourth step of fixing the fluorescence-peptide substrate-quencher-polymers onto a 96-well.
Abstract translation: 目的：提供用于定量测定蛋白酶活性的体外试剂盒及其制备方法，以特异性发射强荧光。 构成：用于定量测定蛋白酶活性的体外试剂盒包括其中结合荧光和猝灭剂并且将肽底物与聚合物缀合的复合物。 络合物具有结构式1.猝灭剂是黑孔淬灭剂，黑莓猝灭剂或其衍生物。 制备试剂盒的方法包括：将特定肽底物合成特定蛋白酶并与近红外线荧光反应的第一步骤; 用猝灭剂结合肽底物的第二步; 将聚合物结合到荧光肽底物猝灭剂的第三步骤; 以及将荧光肽底物淬灭聚合物固定在96孔上的第四步骤。
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Patent Agency Ranking
公开(公告)号：KR102228272B1
公开(公告)日：2021-03-17
申请号：KR1020190145221
申请日：2019-11-13
Applicant: 한국과학기술연구원
Abstract: 본발명은항암상승효과를나타내는종양세포특이적자기조립나노약물복합체에관한것으로, 보다구체적으로는종양세포에존재하는효소에의해서분해되어, 활성성분인항암약물및 약물내성억제펩타이드(SMAC)를동시에방출할수 있는나노약물복합체에관한것이다. 본발명에따른약물복합체또는자기조립나노입자는종양조직에서특이적으로과발현하는카텝신 B 효소에의해분해되어, 독소루비신및 SMAC을방출하는프로드러그(prodrug)로, 종양세포에특이적으로반응하여활성화되기때문에, 암예방또는치료과정에서유도되는심각한정상조직손상및 독성등의부작용을해결할수 있다. 또한, 본발명에따른약물복합체또는자기조립나노입자는수성상태에서추가적인고분자및 전달체없이안정한나노입자를형성하므로기존의고분자및 전달체기반의약물전달제형보다높은약물봉입및 전달효율, 대량합성및 QC가용이하다. 또한, 본발명에따른약물복합체또는자기조립나노입자는독소루비신및 SMAC을암 세포에방출하여상승효과에의해우수한항암효능을나타낼뿐만아니라, 기존항암화학치료의문제였던약물내성을극복할수 있다.
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公开(公告)号：KR101467825B1
公开(公告)日：2014-12-04
申请号：KR1020120112522
申请日：2012-10-10
Applicant: 한국과학기술연구원
Abstract: 본발명은표피생장인자수용체 (EGFR)-양성종양표적화소광나노프로브및 이의제조방법에관한것으로서, 더욱구체적으로는, 표피생장인자 (EGF)에, 형광체및 소광체가컨주게이션된것을특징으로하는표피생장인자수용체 (EGFR)-양성종양표적화소광나노프로브및 이의제조방법에관한것이다. 본발명에따른표피생장인자수용체 (EGFR)-양성종양표적화소광나노프로브 (EGF-NP)는표피생장인자수용체-양성종양을특이적으로검출할수 있으며, 따라서표피생장인자수용체-표적화된약물의치료효과를모니터링하는데매우효과적이다. 또한, 짧은영상화시간을갖기때문에, 반복적인영상획득을쉽게간단하게수행할수 있도록하며, 시간경과에따른연속적영상데이터를획득하는데유용하다. 더불어, 본발명에따른 EGF-NP는소정시간경과후 체내에서소멸되기때문에, 신체안전성측면에서도우수하다.
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公开(公告)号：KR101452775B1
公开(公告)日：2014-10-21
申请号：KR1020120090911
申请日：2012-08-20
Applicant: 한국과학기술연구원
Abstract: 본 발명은 단백질 분해 효소 활성의 정량적 측정용 시험관내 키트 및 그 제조방법에 관한 것이다. 보다 상세하게는, 형광체 및 소광체가 결합되고, 단백질 분해효소에 의하여 특이적으로 분해되는 펩타이드 기질이 고분자에 결합된 복합체를 포함하는 시험관내 단백질 분해효소 활성의 정량적 측정용 키트에 관한 것이다.
본 발명에 따른 복합체는 소광체의 형광물질에 대한 높은 소광 능력으로 형광이 발광하지 않은 상태로 있다가 특정 단백질 분해효소에 의하여 펩타이드 기질이 분해가 되어야만 강한 형광을 특이적으로 발광함으로써, 특히 단백질 분해효소 과다 발현을 억제하는 억제제와 같은 신약을 스크리닝하는 방법, 암, 골 관절염, 류마티스 관절염, 치매 등 자가면역질환 등의 다양한 질병 및 난치병의 조기 진단에 유용하게 사용될 수 있다.-
公开(公告)号：KR1020140072273A
公开(公告)日：2014-06-13
申请号：KR1020120137013
申请日：2012-11-29
Applicant: 한국과학기술연구원
CPC classification number: A61K9/127 , C12N15/88 , C12Q1/686 , A61K47/50 , A61K48/00 , C12Q2563/159 , C12Q2563/161
Abstract: The present invention relates to a gene delivery carrier for effectively delivering a gene to cells without cytotoxicity and a method for preparing the same and, more specifically, to a PCR-based gene delivery carrier which comprises: a shell composed of neutral liposomes; and template DNA and PCR components including polymerase, dNTPs, and primers for amplification of the template DNA by PCR within an inner space defined by the shell. According to the present invention, the gene delivery carrier with improved gene loading efficiency without cytotoxicity and the method for preparing the same are provided.
Abstract translation: 本发明涉及一种将基因有效递送至无细胞毒性的细胞的基因递送载体及其制备方法，更具体地涉及基于PCR的基因递送载体，其包含：由中性脂质体组成的壳; 以及模板DNA和PCR组分，包括聚合酶，dNTP和引物，用于通过PCR在壳内限定的内部空间内扩增模板DNA。 根据本发明，提供了具有改善的基因负载效率而没有细胞毒性的基因递送载体及其制备方法。
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公开(公告)号：KR1020140024193A
公开(公告)日：2014-02-28
申请号：KR1020120090911
申请日：2012-08-20
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/37 , G01N15/14 , G01N33/56972 , G01N33/582 , G01N2800/102
Abstract: The present invention relates to an in vitro kit for quantitatively detecting protease activity and a method for manufacturing the same and more specifically, to an in vitro kit comprising a complex, in which a fluorescent substance and a quencher are combined and a peptide substrate specifically degraded by the protease is combined with a polymer nanoparticle, for quantitatively detecting protease activity. Since the complex according to the present invention has a high quenching effect against the fluorescent material of a quencher, the complex specifically emits strong fluorescence if the peptide substrate is degraded by a certain protease, while the complex emits no fluorescence if not, thereby being able to be useful for screening of novel drugs such as an inhibitor suppressing the overexpression of the protease and early diagnosis with various diseases like autoimmune diseases such as cancer, ostarthritis, rheumatoid arthritis, Alzheimer's disease, etc. and incurable disease. [Reference numerals] (AA) In case of using linear polymer; (BB) In case of using nanoparticles; (CC) Extinction state (-) enzyme; (DD) Restoration state (+) enzyme 5 min; (EE) Restoration state (+) enzyme 60 min; (FF) Difference in contrast; (GG) 170 fold; (HH) 81 fold
Abstract translation: 本发明涉及一种用于定量检测蛋白酶活性的体外试剂盒及其制备方法，更具体地说，涉及一种体外试剂盒，其包含组合物，其中荧光物质和猝灭剂组合，并且肽底物特异性降解 通过蛋白酶与聚合物纳米颗粒结合，用于定量检测蛋白酶活性。 由于根据本发明的复合物对猝灭剂的荧光材料具有高淬灭效果，所以如果肽底物被某些蛋白酶降解，则复合物特异性地发射强荧光，而如果不能，复合物不发出荧光，从而能够 可用于筛选抑制蛋白酶过表达的抑制剂和诸如癌症，骨关节炎，类风湿性关节炎，阿尔茨海默氏病等自身免疫性疾病等各种疾病的早期诊断等新药。 （标号）（AA）使用线性聚合物的情况; （BB）使用纳米粒子时; （CC）消光状态（ - ）酶; （DD）恢复状态（+）酶5分钟; （EE）恢复状态（+）酶60分钟; （FF）对比差异 （GG）170倍; （HH）81折
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