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    심장 부정맥 진단용 바이오마커 및 이를 이용한 심장 부정맥 진단 방법
    26.
    发明公开
    심장 부정맥 진단용 바이오마커 및 이를 이용한 심장 부정맥 진단 방법 有权
    用于诊断心脏病的生物标志物及其诊断方法

    公开(公告)号：KR1020130036588A

    公开(公告)日：2013-04-12

    申请号：KR1020110100761

    申请日：2011-10-04

    Applicant: 한국과학기술연구원

    Inventor： 송은주 ,  다스타누자 ,  유영숙

    IPC: G01N33/68 ,  G01N33/53

    Abstract: PURPOSE: A bio-marker for diagnosing cardiac arrhythmia and a cardiac arrhythmia diagnosing method using the same are provided to be used as a tool for examining the onset of the cardiac arrhythmia by using a biological sample. CONSTITUTION: A bio-marker for diagnosing the cardiac arrhythmia comprises at least one of selected proteins below, and relatively increases the expression of cardiac muscle cells of the cardiac arrhythmia onset compared to normal cardiac muscle cells. The below group comprises ATP synthase D-chain having an amino acid sequence in sequence number 1, mitochondrial, proteasome beta 4-subunit having an amino acid sequence in sequence number 2, myosin light chain-2 having an amino acid sequence in sequence number 3, peripherin having an amino acid sequence in sequence number 4, glutaredoxin-3 having an amino acid sequence in sequence number 5, cytochrome b-c1 complex subunit-1 having an amino acid sequence in sequence number 6, and Nogo-A having an amino acid sequence in sequence number 7.

    Abstract translation: 目的：提供用于诊断心律失常的生物标志物和使用其的心律失常诊断方法，以用作通过使用生物样品检查心律失常发作的工具。 构成：用于诊断心律失常的生物标志物包含下列选定蛋白质中的至少一种，并且与正常心肌细胞相比，心脏心律不齐发作的心肌细胞的表达相对增加。 下面的组包括具有序列号1的氨基酸序列的ATP合成酶D链，具有序列号2中的氨基酸序列的线粒体，蛋白酶体β4亚基，具有序列号3的氨基酸序列的肌球蛋白轻链-2 ，具有序列号4的氨基酸序列的外周蛋白，具有序列号5的氨基酸序列的谷氧还蛋白-3，具有序列号6的氨基酸序列的细胞色素b-c1复合子亚基-1和具有氨基酸的氨基酸A 序列号7中的酸序列。

    개선된 시료 주입법을 이용한 모세관 전기영동의 검출 감도향상 방법
    27.
    发明公开
    개선된 시료 주입법을 이용한 모세관 전기영동의 검출 감도향상 방법 失效
    使用改进样品注射模式增强毛细管电泳检测灵敏度的方法

    公开(公告)号：KR1020100024107A

    公开(公告)日：2010-03-05

    申请号：KR1020080082804

    申请日：2008-08-25

    Applicant: 한국과학기술연구원

    Inventor： 오을식 ,  유영숙 ,  송은주 ,  나비울

    IPC: G01N33/50 ,  G01N27/447

    Abstract: PURPOSE: A method for improving the detection sensitivity of capillary electrophoresis is provided to control the width and concentration of a sample and to improve detection sensitivity. CONSTITUTION: A method for improving the detection sensitivity of capillary electrophoresis comprises: a step of injecting a running buffer to a capillary(step 1); a step of injecting pH 11.0-13.0 to form a base area(step 2); a step of injecting a sample to be measured(step 3); a step of re-injecting the running buffer of step 1(step 4); and a step of detecting a sample stacked through steps 1 to 4(step 5). The running buffer of step 1 is an organic acid such as formic acid. The base of step 2 is a base such as ammonium hydroxide. The sample is a sample of which a charge amount changes based on the pH of a solvent.

    Abstract translation: 目的：提高毛细管电泳检测灵敏度的方法，以控制样品的宽度和浓度，提高检测灵敏度。 构成：改善毛细管电泳检测灵敏度的方法包括：将运行缓冲液注入毛细管的步骤（步骤1）; 注入pH 11.0-13.0以形成基区的步骤（步骤2）; 注射待测样品的步骤（步骤3）; 重新注入步骤1的运行缓冲器的步骤（步骤4）; 以及检测通过步骤1〜4堆叠的样品的步骤（步骤5）。 步骤1的运行缓冲液是有机酸如甲酸。 步骤2的碱是碱如氢氧化铵。 样品是电荷量基于溶剂的pH而变化的样品。

    신경세포에서 신경돌기 생성을 유도하는 방법
    28.
    发明授权
    신경세포에서 신경돌기 생성을 유도하는 방법 失效
    神经细胞神经元诱导方法

    公开(公告)号：KR100838826B1

    公开(公告)日：2008-06-17

    申请号：KR1020070006241

    申请日：2007-01-19

    Applicant: 한국과학기술연구원

    Inventor： 송은주 ,  유영숙

    IPC: C12N5/07 ,  C12N5/071 ,  C12N5/00

    Abstract: A method for inducing generation of neurite from a neuronal cell is provided to use a proteasome inhibitor for inducing the neurite generation of the neuronal cell by directly activating a TrkA(tropomyosin-related kinase A) receptor, thereby being usefully used in order to study a generation process of the neurite, improving healing of various kinds of neuronal disease or the neuronal cell damage or treating neuron damage. A method for inducing generation of neurite from a neuronal cell comprises the steps of: (a) phosphorylating a TrkA receptor in the neurite with MG132(carbobenzoxy-L-leucyl-L-leucyl-L-leucinal, Z-LLL-CHO) as a proteasome inhibitor; (b) allowing the activated TrkA receptor to phosphorylate MAP(mitogen-activated protein) kinase and AKT(protein kinase B); and (c) inducing transcription of a purine-rich DNA by activating Elk-1(ETS domain containing protein) using the phosphorylated kinase to promote the growth of the neurite, wherein the concentration of the proteasome inhibitor is 0.7-10muM.

    Abstract translation: 提供从神经元细胞诱导神经突的方法，以使用蛋白酶体抑制剂通过直接激活TrkA（原肌球蛋白相关激酶A）受体来诱导神经元细胞的神经突生成，由此有用地用于研究 神经突的产生过程，改善各种神经元疾病的愈合或神经元细胞损伤或治疗神经元损伤。 用于诱导神经元细胞产生神经突的方法包括以下步骤：（a）用MG132（苄氧羰基-L-亮氨酰-L-亮氨酰-L-亮氨酰-L-亮氨酸，Z-LLL-CHO）将神经突中的TrkA受体磷酸化为 蛋白酶体抑制剂; （b）使活化的TrkA受体磷酸化MAP（丝裂原活化蛋白）激酶和AKT（蛋白激酶B）; 和（c）通过使用磷酸化激酶激活Elk-1（含有ETS结构域的蛋白质）诱导富含嘌呤的DNA的转录，以促进神经突的生长，其中蛋白酶体抑制剂的浓度为0.7-10muM。

    생화학 경로의 모델링과 시뮬레이션 방법 및 시스템
    29.
    发明公开
    생화학 경로의 모델링과 시뮬레이션 방법 및 시스템 无效
    用于建模和模拟生物化学途径的方法和系统

    公开(公告)号：KR1020060098657A

    公开(公告)日：2006-09-19

    申请号：KR1020050017886

    申请日：2005-03-03

    Applicant: 한국과학기술연구원

    Inventor： 슈레쉬바부 ,  전수진 ,  송은주 ,  유영숙

    IPC: G06F17/00 ,  C12Q1/68 ,  G06F9/455

    CPC classification number: G06F19/12

    Abstract: 본 발명은 생화학 경로의 모델링과 시뮬레이션 방법 및 시스템에 관한 것으로서, 더욱 상세하게는 생물 시스템의 신호 전달 경로에 포함되는 단백질 농도 등의 생물학적 데이터를 변수로 한 수학적인 동력학 모델을 개발하고 이를 이용하여 시뮬레이션 함으로써 다양한 환경에서 단백질의 기능과 활성화 상태, 단백질 사이의 상호작용 및 신호 전달 경로 등을 예상할 수 있어 신호 전달 네트워크의 정량적 및 정성적 이해가 가능한, 생화학 경로의 모델링과 시뮬레이션 방법 및 시스템에 관한 것이다. 본 발명의 시뮬레이션 모델을 이용하면 약제 설계 등 특정 목적을 위한 신물질 발굴에 있어 실험의 횟수를 최소화할 수 있고 그 적용을 쉽게 예측함으로써 품질을 높일 수 있다.
    동력학 모델, 신경 전달 경로, 시뮬레이션, 단백질 농도, 반응속도식, 효소반응

    효소에 의한 단백질 인산화 분석 방법
    30.
    发明授权
    효소에 의한 단백질 인산화 분석 방법 有权
    효소에의한단백질인산화분석방법

    公开(公告)号：KR100451594B1

    公开(公告)日：2004-10-08

    申请号：KR1020020006554

    申请日：2002-02-05

    Applicant: 한국과학기술연구원

    Inventor： 유영숙 ,  남희선 ,  반은미 ,  유은아 ,  이세윤

    IPC: C12Q1/48

    Abstract: PURPOSE: A determination method of protein phosphorylation reaction by enzyme activity is provided, thereby easily and rapidly analyzing the protein phosphorylation reaction. CONSTITUTION: A determination method of protein phosphorylation reaction by enzyme activity comprises the steps of: determining the optimal pH and concentration of a buffer solution for phosphorylation reaction of myelin basic protein(MBP) by extracellular signal-regulated kinase(ERK); adding the optimal concentration of compound to the buffer solution in order to inhibit the adsorption of peptide to the inner wall of a capillary tube and increase the detection sensitivity; determining MBP peptide from the active region of MBP peptide by ERK, -PRTP- as a center for effectively carrying out the phosphorylation reaction by ERK under the same condition; detecting the phosphorylation reaction of MBP peptide by ERK using a capillary tube electrophoresis using the buffer solution; and additionally confirming the phosphorylation reaction detected using a matrix-supported laser removing/ionizing mass analyzer.

    Abstract translation: 目的：提供一种通过酶活性进行蛋白质磷酸化反应的测定方法，从而可以容易且快速地分析蛋白质磷酸化反应。 构成：通过酶活性确定蛋白质磷酸化反应的方法包括以下步骤：通过细胞外信号调节激酶（ERK）确定用于磷酸化髓磷脂碱性蛋白（MBP）反应的缓冲溶液的最佳pH和浓度; 将最佳浓度的化合物添加到缓冲液中以抑制肽吸附到毛细管内壁并提高检测灵敏度; 通过ERK以-PRTP-从MBP肽的活性区域测定MBP肽作为用于在相同条件下通过ERK有效进行磷酸化反应的中心; 使用缓冲溶液使用毛细管电泳检测由ERK检测MBP肽的磷酸化反应; 并且另外确认使用基质支持的激光去除/电离质量分析仪检测到的磷酸化反应。

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