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公开(公告)号：KR101277696B1
公开(公告)日：2013-06-24
申请号：KR1020110042826
申请日：2011-05-06
Applicant: 한국식품연구원
IPC: G01N33/569 , G01N33/553 , G01N33/52 , C12Q1/24
Abstract: 본 발명은 타겟팅-금 자성 나노입자 및 이의 용도에 관한 것이다. 본 발명의 나노입자는 (ⅰ) 자성물질을 포함하는 코어, (ⅱ) 금을 포함하는 쉘 및 (ⅲ) 상기 쉘에 결합된 타겟팅 모이어티(targeting moiety)로 이루어져 있으며, 상기 쉘에 결합된 타겟팅 모이어티(예컨대, 타겟 미생물의 표면 항원에 대한 항체)를 이용하여 타겟 미생물을 효과적으로 분리, 농축 및 검출을 원-스텝으로 할 수 있다. 따라서, 본 발명의 자성 나노입자-기반된 방법은 종래의 복잡하고 시간-소모적인 방법들에 비해 미생물의 분리 및 농축을 매우 간편하게 실시할 수 있을 뿐 아니라, 동시에 효율적인 검출도 할 수 있다.
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公开(公告)号：KR1020120124902A
公开(公告)日：2012-11-14
申请号：KR1020110042826
申请日：2011-05-06
Applicant: 한국식품연구원
IPC: G01N33/569 , G01N33/553 , G01N33/52 , C12Q1/24
Abstract: PURPOSE: A method for isolating, concentrating, and detecting targeting-gold magnetic nanoparticles and gold magnetic nanoparticle-based microorganisms in one-step is provided to simultaneously detect target microorganisms in a sample. CONSTITUTION: A method for isolating, concentrating, and detecting microorganisms using gold magnetic nanoparticles comprises: a step of contacting a sample targeting-gold magnetic nanoparticles comprising a core, a shell, and a targeting moiety; and a step of applying the resultant to a column. The magnet is applied by a permanent magnet or electromagnet. The targeting moiety is an antibody or a fragment thereof.
Abstract translation: 目的：提供一步法分离，浓缩和检测靶向金磁性纳米颗粒和金磁性纳米粒子微生物的方法，以同时检测样品中的目标微生物。 构成：使用金磁性纳米颗粒分离，浓缩和检测微生物的方法包括：使包含芯，壳和靶向部分的样品靶向金磁性纳米颗粒接触的步骤; 以及将结果应用于柱的步骤。 磁铁由永久磁铁或电磁铁施加。 靶向部分是抗体或其片段。
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23.发明公开식중독균 검출을 위한 자성 코어 금 나노입자 및 그제조방법, 상기 나노입자를 이용한 식중독균 검출방법 有权用于检测食物杆状病毒的磁性金纳米颗粒的制造方法，以及使用磁性金纳米颗粒检测方法食物杆状病毒
公开(公告)号：KR1020090116142A
公开(公告)日：2009-11-11
申请号：KR1020080041885
申请日：2008-05-06
Applicant: 한국식품연구원
CPC classification number: G01N33/56916 , B82Y5/00 , B82Y15/00 , G01N33/543 , G01N33/54326 , G01N33/54346 , G01N33/553
Abstract: PURPOSE: A manufacturing method of magnetic gold nanoparticles for detecting foodborne pathogens is provided to increase reaction activity of foodborne pathogens to separate or concentrate the foodborne pathogens. CONSTITUTION: A manufacturing method of magnetic gold nanoparticles for detecting foodborne pathogens comprises the following steps of: preparing a magnetic core(11) including magnetic materials; modifying the surface of the magnetic core with amine groups; and forming a gold nanoparticle layer(13) on the surface of the modified magnetic core. The magnetic core represents Fe2O3 or Fe3O4. The magnetic core is obtained as follows. NaOH, FeCl2·4H2O, Fe3Cl6·4H2O, and HCl are mixed and stirred by a mechanical stirrer. The supernatant is separated using a permanent magnet and neutralized with HCl. The obtained product is washed with distilled water and ethanol, and dispersed in the ethanol.
Abstract translation: 目的：提供用于检测食源性病原体的磁性金纳米颗粒的制造方法，以提高食源性病原体分离或浓缩食源性病原体的反应活性。 构成：用于检测食源性病原体的磁性金纳米颗粒的制造方法包括以下步骤：制备包括磁性材料的磁芯（11）; 用胺基改性磁芯表面; 以及在所述改性磁芯的表面上形成金纳米颗粒层（13）。 磁芯代表Fe2O3或Fe3O4。 磁芯如下获得。 NaOH，FeCl 2·4H 2 O，Fe 3 Cl 6·4H 2 O和HCl通过机械搅拌器混合并搅拌。 使用永磁体分离上清液并用HCl中和。 所得产物用蒸馏水和乙醇洗涤，分散在乙醇中。
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24.发明公开
公开(公告)号：KR1020090106817A
公开(公告)日：2009-10-12
申请号：KR1020080032185
申请日：2008-04-07
Applicant: 한국식품연구원
IPC: G01N33/52 , G01N33/53 , G01N33/533 , G01N33/02
Abstract: PURPOSE: A zearalenone-fluorescence tracer for detecting zearalenone and a method for manufacturing method thereof are provided to use widely through massive production. CONSTITUTION: A method for manufacturing a zearalenone-fluorescence tracer for detecting zearalenone through fluorescence-polarized light comprises: a step of inducing oxime group to ketone group in molecular structure of the zearlenone to produce a zearalenone-oxime; a step of reacting a linker compound to synthesize fluorescein derivative; and a step of mixing the synthesized fluorescein derivative to the zearalenone-oxime and maintaining at room temperature for two hours.
Abstract translation: 目的：提供用于检测玉米赤霉烯酮的玉米赤霉烯酮 - 荧光示踪剂及其制备方法，以通过大量生产广泛使用。 构成：通过荧光偏振光检测玉米赤霉烯酮的玉米赤霉烯酮 - 荧光示踪剂的制造方法，其特征在于，包括：在所述锆石的分子结构中诱导肟基与酮基的步骤，生成玉米赤霉烯酮肟; 使接头化合物反应合成荧光素衍生物的步骤; 以及将合成的荧光素衍生物与玉米赤霉烯酮肟混合并在室温下维持2小时的步骤。
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公开(公告)号：KR1020090032784A
公开(公告)日：2009-04-01
申请号：KR1020070098279
申请日：2007-09-28
Applicant: 한국식품연구원
IPC: C12P21/08
CPC classification number: C12N15/815 , C07K16/44 , C07K2317/622
Abstract: A method for preparing single-chain antibody against zearalenone using recombinant methylotrophic yeast is provided to produce stabilized antibody with excellent expression rates, solubility and antigen affinity. A method for preparing single-chain antibody against zearalenone using recombinant methylotrophic yeast comprises the following steps of: amplifying single-chain antibody against zearalenone using a PCR(polymerase chain reaction) method; cloning the amplified single-chain antibody against zearalenone to an expression vector and verifying existence of the cloning; transforming the cloned expression vector to methylotrophic yeast and selecting recombinant yeast; and expressing single-chain antibody against zearalenone among selected recombinant yeast.
Abstract translation: 提供使用重组甲基营养酵母制备针对玉米赤霉烯酮的单链抗体的方法以产生具有优异表达速率，溶解度和抗原亲和力的稳定的抗体。 使用重组甲基营养酵母制备针对玉米赤霉烯酮的单链抗体的方法包括以下步骤：使用PCR（聚合酶链式反应）方法扩增针对玉米赤霉烯酮的单链抗体; 将扩增的针对玉米赤霉烯酮的单链抗体克隆到表达载体并验证克隆的存在; 将克隆的表达载体转化为甲基营养酵母并选择重组酵母; 并在选择的重组酵母中表达针对玉米赤霉烯酮的单链抗体。
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Patent Agency Ranking
公开(公告)号：KR102121817B1
公开(公告)日：2020-06-26
申请号：KR1020180108713
申请日：2018-09-12
Applicant: 한국화학연구원 , 한국식품연구원 , 한양대학교 산학협력단 , 고려대학교 산학협력단
IPC: C12N15/67 , C12N15/10 , C12N15/90 , C12N9/22 , C12N15/113 , C07K14/005
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公开(公告)号：KR101738391B1
公开(公告)日：2017-05-23
申请号：KR1020130047349
申请日：2013-04-29
Applicant: 한국식품연구원
IPC: G01N21/00
Abstract: 본발명의일 실시예와관련된스캐닝(scanning) 모듈은광원으로부터입사되는전자기파의경로를변경시키는제 1 경로변경부와, 상기제 1 경로변경부를이동시켜상기전자기파의경로를조절하는제 1 구동부및, 상기제 1 경로변경부에의해경로가변경된전자기파를이용하여피검물의적어도일부분에베셀빔이형성되도록하는베셀빔 형성부를포함포함한다.
Abstract translation: 涉及本发明的一个实施例中扫描（扫描）模块从第一路径改变单元，用于改变所述的电磁波作为从光源单元入射的改变所述第一路径的第一驱动单元，用于控制电磁波的路径的路径移动，并且 它包括包括波束形成，使得贝塞尔形成在检查对象的带有电磁波路径至少部分光束的第一容器通过的路径改变部分改变。
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公开(公告)号：KR1020170037909A
公开(公告)日：2017-04-05
申请号：KR1020170036281
申请日：2017-03-22
Applicant: 한국식품연구원
IPC: A61K36/752 , A23K10/30 , A23K20/195
CPC classification number: A61K36/752 , A23K10/30 , A23K20/195
Abstract: 본발명은유자추출물을유효성분으로포함하는정족수인식억제용조성물에관한것이다. 본발명에따른유자추출물은독성이없으며, 미생물간의의사소통을방해하는정족수인식억제활성이있어, 미생물의번식과생물막의형성등을효과적으로차단한다. 따라서본 발명의조성물은미생물의감염을차단하거나생물막의형성을방지할필요가있는각종생활도구및 의료기구등에다양하게이용될수 있으며, 항균제및 미생물에의해발생하는질병의치료제또는사료첨가제로유용하게사용될수 있다.
Abstract translation: 本发明涉及用于抑制含柑桔皮提取物作为活性成分的法定量水的组合物。 没有按照本发明提取物香橼毒性，存在阻止微生物，有效地阻止这样形成的微生物和生物膜生长的群体感应的抑制活性之间的通信。 因此，本发明的组合物可以不同地用于阻断感染的微生物或各种工具寿命的，并且在医疗装置，有必要防止生物膜的形成，可用于治疗或饲料添加剂引起的杀生物剂和微生物疾病的 可以使用。
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公开(公告)号：KR1020170034017A
公开(公告)日：2017-03-28
申请号：KR1020150132135
申请日：2015-09-18
Applicant: 한국식품연구원
IPC: G01N21/31 , G01N21/3586
Abstract: 본발명의일 실시예와관련된전자기파변환모듈은경로변경부로부터입사되는전자기파를검사대상물체로집속시키는스캐닝렌즈및 상기검사대상물체를투과한전자기파의단면적의크기를, 검출부가검출할수 있는단면적의크기와같거나작게변환시키는단면적변환부;를포함한다.
Abstract translation: 涉及本发明的实施例的电磁转换的横截面的模块，它可以通过扫描透镜透射的光的横截面尺寸和用于聚焦的电磁波入射从副路径改变到所述电磁波检测部检测所述试样中的试样 以及截面积转换单元，用于将截面积转换单元转换成等于或小于该尺寸的尺寸。
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