Abstract:
PURPOSE: A novel Micrococcus alkanovora SL-010 with lipid lysis ability is provided to live even at high temperature, acid, and salinity. CONSTITUTION: A Micrococcus alkanovora SL-010(deposite number: KCTC 18130P) has stress resistance and high lipid lysis ability. The Micrococcus alkanovora SL-010(deposite number: KCTC 18130P) is isolated from insect(scolopendra subspinipes). A gene(DNA) which encodes lipid lysis enzyme is derived from Micrococcus alkanovora SL-010(deposite number: KCTC 18130P).
Abstract:
PURPOSE: A probe for detecting bacteria causing food poisoning, Cronobacter sakazakii, and a DNA chip containing the same are provided to quickly and accurately detect Cronobacter sakazakii. CONSTITUTION: A probe for detecting bacteria causing food poisoning, Cronobacter sakazakii contains one or more among sequence lists 40-50. A DNA chip for detecting Cronobacter sakazakii contains the probe and quickly and accurately detects Cronobacter sakazakii. Cronobacter sakazakii is prepared from ATCC(American Type Culture Collection).
Abstract:
PURPOSE: A probe for detecting Staphylococcus aureus and a DNA chip containing the same are provided to quickly and accurately detect Staphylococcus aureus. CONSTITUTION: An oligonucleotide probe for a DNA chip for detecting bacteria causing foot poisoning, Staphylococcus aureus, contains one or more among sequence lists 25-40. The DNA chip contains the probe. The oligonucleotide probe quickly and accurately detects Staphylococcus aureus.
Abstract:
PURPOSE: A probe for detecting bacteria causing food poisoning, Campylobacter jejuni, and a DNA chip containing the same are provided to quickly and accurately detect Campylobacter jejuni. CONSTITUTION: An oligonucleotide probe for a DNA chip for detecting Campylobacter jejuni contains one or more among sequence lists 26-50. The DNA chip contains the probe. The oligonucleotide probe quickly and accurately detects bacteria causing food poisoning, Campylobacter jejuni.
Abstract:
PURPOSE: A diagnosis method of virus using RAPD method is provided to simply and efficiently diagnose virus which do not have index gene which informs phylogenic location. CONSTITUTION: A diagnosis method of virus uses RAPD method(random amplified polymorphic DNA). A diagnosis method of virus comprises the following steps: amplifying DNA pieces of virus by applying RAPD PCR to virus which will be diagnosed; and comparing banding patterns of band by performing electrophoresis the amplified DNA pieces. A random primer manufacturing method used in RAPD PCR comprises the following steps: manufacturing primers comprised of 10 nucleotides by varying G+C content of primer base sequence and
Abstract:
A collection and detection method of microorganism in food through ultrafiltration is provided to analyze various kinds of microorganism in a small amount of sample and reduce time and expense for analysis of microorganism. A microorganism in food is collected and concentrated through ultrafiltration using hollow-fiber. The hollow-fiber is pretreated with bovine calf serum, NaPP, glycine-added FBS, beef extract, bovine serum albumin (BSA), or nutrient broth blocking solution. The microorganism is bacteriophage, virus, germ, prokaryote or spore. The virus is rotavirus or noroviurs. The microorganism is detected by using PCR (polymerase chain reaction) of collected and concentrated microorganism sample.
Abstract:
PURPOSE: A kit for detecting Giardia lamblia in a test sample is provided to effectively detect or diagnose Giardia lamblia causing digestive disorders. CONSTITUTION: A kit for detecting Giardia lamblia contains a primer or probe which specifically binds to a nucleotide sequence of sequence number 2 or 3. The kit is a kit for amplifying a gene containing the primer pair of sequence numbers 6 and 7 or 8 and 9. The kit is a microarray including the probe having a nucleotide sequence selected from sequence numbers 6-9.
Abstract:
PURPOSE: A kit for specifically detecting Giardia lamblia, Cryptosporidium parvum and Entamoeba histolytica is provided to specifically detect protozoa causing diseases. CONSTITUTION: A kit for specifically detecting Giardia lamblia has a primer or a probe which specifically binds to a nucleotide of sequence number 13 or 14. A kit for specifically detecting Cryptosporidium parvum contains a primer or a probe which specifically binds to a nucleotide sequence of sequence number 15 or 16. A kit for specifically detecting Entamoeba histolytica contains a primer or a probe which specifically binds to a nucleotide sequence of sequence number 17 or 18.
Abstract:
본 발명은 수인성 감염으로 인해 질병을 유발시킬 수 있는 주요 원생동물로 알려진 람블편모충( Giardia lamblia ), 작은와포자충( Cryptosporidium parvum ) 및 이질아메바( Entamoeba histolytica )의 검출용 키트에 관한 것으로, 특이적으로 결합하는 프라이머 또는 프로브를 포함하는 람블편모충, 작은와포자충 및 이질아메바를 특이적으로 검출하기 위한 키트에 관한 것이다. 본 발명에 따르면, 본 발명은 람블편모충, 작은와포자충 및 이질아메바를 특이적을 검출할 수 있는 기술을 제공한다. 바람직하게는, 본 발명은 멀티플렉스 PCR을 통해 람블편모충, 작은와포자충 및 이질아메바를 동시 검출할 수 있는 키트를 제공 할 수 있다.