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公开(公告)号：KR1020130046328A
公开(公告)日：2013-05-07
申请号：KR1020110111453
申请日：2011-10-27
Applicant: 한국외국어대학교 연구산학협력단
CPC classification number: Y02A50/451 , C12Q1/689 , C12Q1/6837 , C12R1/63
Abstract: PURPOSE: A probe for detecting bacteria causing food poisoning, Vibrio parahaemolyticus, and a DNA chip containing the same are provided to quickly and accurately detect Vibrio parahaemolyticus. CONSTITUTION: A probe for detecting bacteria causing food poisoning, Vibrio parahaemolyticus, contains one or more among sequence lists 29-50. A DNA chip for detecting Vibrio parahaemolyticus contains the probe. The DNA chip is used for quick and accurate detection of Vibrio parahaemolyticus.
Abstract translation: 目的：提供用于检测引起食物中毒的细菌，副溶血弧菌和含有其的DNA芯片的探针，以快速准确地检测副溶血弧菌。 构成：用于检测引起食物中毒的细菌，副溶血弧菌的探针在序列表29-50中含有一个或多个。 用于检测副溶血弧菌的DNA芯片含有探针。 DNA芯片用于快速准确检测副溶血弧菌。
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公开(公告)号：KR1020130046327A
公开(公告)日：2013-05-07
申请号：KR1020110111452
申请日：2011-10-27
Applicant: 한국외국어대학교 연구산학협력단
CPC classification number: Y02A50/451 , C12Q1/689 , C12Q1/6837 , C12R1/63
Abstract: PURPOSE: A probe for detecting bacteria causing food poisoning, Vibrio vulnificus, and a DNA chip containing the same are provided to quickly and accurately detect Vibrio vulnificus. CONSTITUTION: A probe for detecting bacteria causing food poisoning, Vibrio vulnificus, contains one or more among sequence lists 40-50. A DNA chip for detecting Vibrio vulnificus contains the probe. The DNA chip is used for quickly and accurately detecting Vibrio vulnificus. The Vibrio vulnificus strain is prepared from ATCC(American Type Culture collection) in the number of (ATCC14035).
Abstract translation: 目的：提供用于检测引起食物中毒的细菌，创伤弧菌和含有其的DNA芯片的探针，以快速准确地检测创伤弧菌。 构成：用于检测引起食物中毒的细菌，创伤弧菌的探针，在序列表40-50中含有一个或多个。 用于检测创伤弧菌的DNA芯片含有探针。 DNA芯片用于快速准确地检测创伤弧菌。 创伤弧菌菌株由ATCC（美国典型培养物保藏中心）制备（ATCC14035）。
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3.发明公开
公开(公告)号：KR1020130046325A
公开(公告)日：2013-05-07
申请号：KR1020110111450
申请日：2011-10-27
Applicant: 한국외국어대학교 연구산학협력단
CPC classification number: C12Q1/689 , C12Q1/6837 , C12R1/01
Abstract: PURPOSE: A probe for detecting bacteria causing food poisoning, Yersinia enterocolitica, and a DNA chip containing the same are provided to quickly and accurately detect Yersinia enterocolitica. CONSTITUTION: A probe for detecting bacteria causing food poisoning, Yersinia enterocolitica, contains one or more among sequence lists 30-50. A DNA chip for detecting Yersinia enterocolitica contains the probe. The probe and the DNA chip quickly and accurately detect Yersinia enterocolitica.
Abstract translation: 目的：提供用于检测引起食物中毒的细菌，小肠结肠炎耶尔森菌和含有其的DNA芯片的探针，以快速准确地检测小肠结肠炎耶尔森菌。 构成：用于检测引起食物中毒的细菌的小肠结肠炎耶尔森菌（Yersinia enterocolitica）的探针包含30-50个序列表中的一个或多个。 用于检测小肠结肠炎耶尔森氏菌的DNA芯片含有探针。 探针和DNA芯片快速准确地检测小肠结肠炎耶尔森菌。
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公开(公告)号：KR1020130046319A
公开(公告)日：2013-05-07
申请号：KR1020110111444
申请日：2011-10-27
Applicant: 한국외국어대학교 연구산학협력단
CPC classification number: C12Q1/689 , C12Q1/6837 , C12R1/085
Abstract: PURPOSE: A probe for detecting Bacillus cereus and a DNA chip containing the same are provided to quickly and accurately detect Bacillus cereus which is bacteria causing foot poisoning. CONSTITUTION: A probe for detecting bacteria causing food poisoning, Bacillus cereus, contains one or more among sequence lists 20-50. A DNA chip for detecting Bacillus cereus contains one or more probes. The DNA chip quickly and accurately detects Bacillus cereus. Bacillus cereus is obtained from ATCC(American Type Culture Collection).
Abstract translation: 目的：提供用于检测蜡状芽孢杆菌的探针和含有它的DNA芯片，以快速准确地检测是引起足部中毒的细菌的蜡状芽孢杆菌。 构成：用于检测引起食物中毒的细菌，蜡样芽孢杆菌的探针，含有20-50个序列表中的一个或多个。 用于检测蜡状芽孢杆菌的DNA芯片含有一种或多种探针。 DNA芯片快速准确地检测蜡状芽孢杆菌。 蜡状芽孢杆菌获自ATCC（American Type Culture Collection）。
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公开(公告)号：KR1020090124769A
公开(公告)日：2009-12-03
申请号：KR1020080051165
申请日：2008-05-30
Applicant: 한국외국어대학교 연구산학협력단 , 국민대학교산학협력단
Abstract: PURPOSE: A method for detecting bacteria causing food poisoning of fruit juice through real time PCR is provided to simply and quickly detect bacteria in fruit juice. CONSTITUTION: A method for detecting bacteria causing food poisoning of fruit juice comprises: a step of performing lysis of fruit juice through ultrasonic wave to prepare sample; a step of adding chelating resin to the sample then centrifuging; a step of purifying the sample through gel filtration column to prepare DNA template; a step of performing realtime PCR with prepared DNA template. The gel filtration column is sephadex column.
Abstract translation: 目的：通过实时PCR检测引起果汁食物中毒的细菌，简单快速地检测果汁中的细菌。 构成：用于检测导致果汁食物中毒的细菌的方法，包括：通过超声波进行果汁溶解以制备样品的步骤; 向样品中加入螯合树脂然后离心的步骤; 通过凝胶过滤柱纯化样品以制备DNA模板的步骤; 用制备的DNA模板进行实时PCR的步骤。 凝胶过滤柱是sephadex柱。
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Patent Agency Ranking
公开(公告)号：KR1020130046326A
公开(公告)日：2013-05-07
申请号：KR1020110111451
申请日：2011-10-27
Applicant: 한국외국어대학교 연구산학협력단
CPC classification number: Y02A50/451 , Y02A50/52 , C12Q1/689 , C12Q1/6837 , C12R1/63
Abstract: PURPOSE: A probe for detecting bacteria causing food poisoning, Vibrio cholerae, a DNA chip containing the same are provided to quickly and accurately detect Vibrio cholerae. CONSTITUTION: A probe for detecting bacteria causing food poisoning, Vibrio cholerae, contains one or more among sequence lists 40-50. A DNA chip for detecting Vibrio cholerae contaisn the probe. The DNA chip is used for quickly and accurately detecting Vibrio cholerae. Vibrio cholerae strain is prepared from ATCC(American Type Culture Collection) in the number ATCC 14035.
Abstract translation: 目的：提供用于检测引起食物中毒的细菌的探针，霍乱弧菌，含有其的DNA芯片，用于快速准确地检测霍乱弧菌。 构成：用于检测引起食物中毒的细菌霍乱弧菌的探针在序列表40-50中含有一个或多个。 用于检测霍乱弧菌的DNA芯片。 DNA芯片用于快速准确检测霍乱弧菌。 霍乱弧菌菌株由编号ATCC 14035的ATCC（美国典型培养物保藏中心）制备。
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公开(公告)号：KR1020130046322A
公开(公告)日：2013-05-07
申请号：KR1020110111447
申请日：2011-10-27
Applicant: 한국외국어대학교 연구산학협력단
CPC classification number: Y02A50/451 , C12Q1/689 , C12Q1/6837 , C12R1/42
Abstract: PURPOSE: A probe for detecting bacteria causing food poisoning, Salmonella enterica, and a DNA chip containing the same are provided to quickly and accurately detect Salmonella enterica. CONSTITUTION: An oligonucleotide probe for a DNA chip for detecting bacteria causing food poisoning, Salmonella enterica, contains one or more among sequence lists 40-50. The DNA chip for detecting Salmonella enterica contains the probe. The DNA chip quickly and accurately detects Salmonella enterica.
Abstract translation: 目的：提供用于检测引起食物中毒的细菌，肠炎沙门氏菌和含有该菌的DNA芯片的探针，以快速准确地检测肠炎沙门氏菌。 构成：用于检测导致食物中毒的细菌的DNA芯片的寡核苷酸探针，肠道沙门氏菌在序列表40-50中含有一个或多个。 用于检测肠炎沙门氏菌的DNA芯片含有探针。 DNA芯片快速，准确地检测肠炎沙门氏菌。
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公开(公告)号：KR100906160B1
公开(公告)日：2009-07-03
申请号：KR1020070058637
申请日：2007-06-15
Applicant: 한국외국어대학교 연구산학협력단
IPC: C12Q1/68
Abstract: 본 발명은 중합효소연쇄반응(polymerase chain reaction)과 효소결합면역반응(enzyme-linked immunosorbent assay)을 이용한 식중독 세균
Listeria
monocytogenes 의 검출법 및 검출키트에 관한 것으로, 더욱 상세하게는
Listeria
monocytogenes 의 병원성 인자(virulence factor)인 리스테리오라이신(listeriolysin)을 코딩하는
hly 유전자를 선택적으로 검출할 수 있는 PCR 프라이머로 중합효소연쇄반응을 시키고, 위 PCR 반응의 산물에 특이적으로 결합할 수 있는 탐침자(capture probe)를 넣어 혼성화시킨 후, 위 혼성화된 PCR 반응의 산물에 효소결합면역반응(ELISA)을 적용시켜서 위 식중독 세균을 검출하는 방법 및 키트에 관한 것이다.
이때, 중합효소연쇄반응에 사용하는 PCR 프라이머의 염기서열은 서열목록 1과 2인 것을 특징으로 한다. 또한 이때, 탐침자(capture probe)의 염기서열은 서열목록 3인 것을 특징으로 한다.
본 발명에 따른 검출검사에 소요되는 시간은 총 5시간 미만이며 휴대가 가능하고 검사절차가 간편하며 검사결과를 육안으로 관찰할 수 있기 때문에 현장 검사용으로도 유용하다 하겠다.
중합효소연쇄반응(PCR), 효소결합면역반응(ELISA), Listeria monocytogenes-
公开(公告)号：KR1020130046323A
公开(公告)日：2013-05-07
申请号：KR1020110111448
申请日：2011-10-27
Applicant: 한국외국어대학교 연구산학협력단
CPC classification number: C12Q1/689 , C12Q1/6837 , C12R1/145
Abstract: PURPOSE: A probe for detecting bacteria causing food poisoning, and a DNA chip containing the same are provided to quickly and accurately detect Clostridium perfringens. CONSTITUTION: A probe for detecting bacteria causing food poisoning, Clostridium perfringens, contains one or more among sequence lists 13-50. A DNA chip for detecting Clostridium perfringens contains the probe. The DNA chip quickly and accurately detects Clostridium perfringens. Clostridium perfringens is prepared from ATCC(American Type Culture Collection).
Abstract translation: 目的：提供用于检测引起食物中毒的细菌的探针和含有此类物质的DNA芯片，以快速准确地检测产气荚膜梭菌。 构成：检测产生食物中毒的细菌产气荚膜梭菌的探针在序列表13-50中含有一个或多个。 用于检测产气荚膜梭菌的DNA芯片含有探针。 DNA芯片快速准确地检测产气荚膜梭菌。 产气荚膜梭菌由ATCC（美国典型培养物保藏中心）制备。
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公开(公告)号：KR1020130013175A
公开(公告)日：2013-02-06
申请号：KR1020110074667
申请日：2011-07-27
Applicant: 한국외국어대학교 연구산학협력단
CPC classification number: C12Q1/689 , C12Q1/6837
Abstract: PURPOSE: An oligonucleotide probe for a DNA chip for detecting Listeria monocytogenes is provided to quickly and accurately detect Listeria monocytogenes which is a food poisoning bacterium. CONSTITUTION: An oligonucleotide probe for a DNA chip to detec Listeria monocytogenes which is a food poisoning bacterium contains one or more sequences among sequence numbers 21-42. The DNA chip contains one or more probes. Listeria monocytogenes is deposited by deposit number ATCC19117. Listeria monocytogenes is sub-cultured in Brain Heart Infusion Agar.
Abstract translation: 目的：提供用于检测单核细胞增生利斯特氏菌的DNA芯片的寡核苷酸探针，以快速准确地检测作为食物中毒细菌的单核细胞增生利斯特氏菌（Listeria monocytogenes）。 构成：用于检测作为食物中毒细菌的单核细胞增生利斯特氏菌的DNA芯片的寡核苷酸探针含有序列号21-42中的一个或多个序列。 DNA芯片包含一个或多个探针。 单核细胞增多性李斯特菌由保藏号ATCC19117保藏。 单核细胞增多性李斯特菌在脑心脏输液琼脂中亚培养。
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