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    SORF CONSTRUCTS AND MULTIPLE GENE EXPRESSION
    21.
    发明专利
    SORF CONSTRUCTS AND MULTIPLE GENE EXPRESSION 未知

    公开(公告)号：CA2776990A1

    公开(公告)日：2011-05-05

    申请号：CA2776990

    申请日：2010-10-28

    Applicant: ABBOTT LAB

    Inventor： CARSON GERALD R ,  GION WENDY R ,  KUNES YUNE Z ,  LEISE WALTER F III ,  DAVIS-TABER RACHEL A ,  FUNG EMMA

    IPC: C07K16/00 ,  C12P21/02 ,  C12P21/06

    Abstract: Embodiments of the invention relate to vector constructs and methods for expression of polypeptides including multimeric products such as therapeutic antibodies. Particular constructs allow for the generation of expression products from a single open reading frame (sORF). An embodiment provides an isolated or purified expression vector for generating one or more recombinant protein products comprising a single open reading frame insert; said insert comprising a signal peptide nucleic acid sequence encoding a signal peptide; a first nucleic acid sequence encoding a first polypeptide; a first intervening nucleic acid sequence encoding a first protein cleavage site, wherein said first protein cleavage site is provided by an intein segment of a Ion protease gene of Pyrococcus or a klbA gene of Pyrococcus or Methanococcus, or a modified intein segment derived therefrom; and a second nucleic acid sequence encoding a second polypeptide. Certain embodiments of constructs and methods employ an intein segment of a Ion protease gene of Pyrococcus abyssi, Pyrococcus furiosus, or Pyrococcus horikoshii OT3; or an intein segment of a klbA gene of Pyrococcus abyssi, Pyrococcus furiosus, or Methanococcus jannaschii; or other intein segment.

    CONSTRUCCIONES DE SORF Y EXPRESION DE GEN MULTIPLE.
    25.
    发明专利
    CONSTRUCCIONES DE SORF Y EXPRESION DE GEN MULTIPLE. 未知

    公开(公告)号：MX2012005117A

    公开(公告)日：2012-06-14

    申请号：MX2012005117

    申请日：2010-10-28

    Applicant: ABBOTT LAB

    Inventor： FUNG EMMA ,  CARSON GERALD R ,  GION WENDY R ,  KUNES YUNE Z ,  LEISE WALTER F III ,  DAVIS-TABER RACHEL A

    IPC: C07K16/00 ,  C12P21/02 ,  C12P21/06

    Abstract: Las modalidades de la invención se refieren a construcciones de vector y a métodos para expresión de polipéptidos incluyendo productos multiméricos tales como anticuerpos terapéuticos. Las construcciones particulares permiten la generación de productos de expresión a partir de un marco de lectura abierto individual (sORF). Una modalidad provee un vector de expresión aislado o purificado para generar uno o más productos de proteína recombinante que comprenden un inserto de marco de lectura abierto individual; dicho inserto comprende una secuencia de ácido nucleico de péptido de señal que codifica para un péptido de señal; una primera secuencia de ácido nucleico que codifica para un primer polipéptido; una primera secuencia de ácido nucleico de intercalado que codifica para un primer sitio de corte de proteína, en la cual dicho primer sitio de corte de proteína es provisto por un segmento de inteína de un gen de proteasa Ion de Pyrococcus o un gen klbA de Pyrococcus o Methanococcus, o un segmento de inteína modificada derivado a partir de los mismos; y una segunda secuencia de ácido nucleico que codifica para un segundo polipéptido. Ciertas modalidades de construcciones y métodos emplean un segmento de inteína de un gen de proteasa Ion de Pyrococcus abyssi, Pyrococcus furiosus, o Pyrococcus horikoshii 0T3; o un segmento de inteína de un gen klbA de Pyrococcus abyssi, Pyrococcus furiosus, o Methanococcus jannaschii; u otro segmento de inteína.

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    26.
    发明专利
    "SORF CONSTRUCTS AND MULTIPLE GENE EXPRESSION" 未知

    公开(公告)号：IN3281DEN2012A

    公开(公告)日：2015-10-23

    申请号：IN3281DEN2012

    申请日：2012-04-16

    Applicant: ABBOTT LAB

    Inventor： CARSON GERALD R ,  GION WENDY R ,  KUNES YUNE Z ,  LEISE III WALTER F ,  DAVIS-TABER RACHEL A ,  FUNG EMMA

    IPC: C07K16/00

    Abstract: Embodiments of the invention relate to vector constructs and methods for expression of polypeptides including multimeric products such as therapeutic antibodies. Particular constructs allow for the generation of expression products from a single open reading frame (sORF). An embodiment provides an isolated or purified expression vector for generating one or more recombinant protein products comprising a single open reading frame insert; said insert comprising a signal peptide nucleic acid sequence encoding a signal peptide; a first nucleic acid sequence encoding a first polypeptide; a first intervening nucleic acid sequence encoding a first protein cleavage site, wherein said first protein cleavage site is provided by an intein segment of a Ion protease gene of Pyrococcus or a klbA gene of Pyrococcus or Methanococcus, or a modified intein segment derived therefrom; and a second nucleic acid sequence encoding a second polypeptide. Certain embodiments of constructs and methods employ an intein segment of a Ion protease gene of Pyrococcus abyssi, Pyrococcus furiosus, or Pyrococcus horikoshii OT3; or an intein segment of a klbA gene of Pyrococcus abyssi, Pyrococcus furiosus, or Methanococcus jannaschir, or other intein segment.

    IL-12/P40 BINDING PROTEINS
    29.
    发明专利
    IL-12/P40 BINDING PROTEINS 未知

    公开(公告)号：SG163559A1

    公开(公告)日：2010-08-30

    申请号：SG2010047561

    申请日：2006-06-29

    Applicant: ABBOTT LAB

    Inventor： LACY SUSAN E ,  FUNG EMMA ,  BELK JONATHAN P ,  DIXON RICHARD W ,  ROGUSKA MICHAEL ,  HINTON PAUL R ,  KUMAR SHANKAR

    Abstract: The present invention encompasses IL-12p40 binding proteins, particularly antibodies that bind human interleukin-12 (hIL-12) and/or human IL-23 (hIL- 23). Specifically, the invention relates to antibodies that are chimeric, CDR grafted and humanized antibodies. Preferred antibodies have high affinity for hIL-12 and/or hIL-23 and neutralize h IL-12 and/or hIL-23 activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. Method of making and method of using the antibodies of the invention are also provided. The antibodies, or antibody portions, of the invention are useful for detecting hIL-12 and/or hIL- 23 and for inhibiting hIL-12 and/or hIL-23 activity, e.g., in a human subject suffering from a disorder in which hIL-12 and/or hIL-23 activity is detrimental.

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