公开(公告)号：CA2592309A1

公开(公告)日：2006-07-20

申请号：CA2592309

申请日：2005-12-20

Applicant: AJINOMOTO KK

Inventor： SUGIYAMA MASAKAZU ,  ABE ISAO ,  SUZUKI EIICHIRO ,  YUUJI REIKO ,  KODAMA YUYA ,  SUZUKI SHUNICHI ,  KASHIWAGI TATSUKI ,  NAKAMURA TAKEFUMI ,  TAKESHITA RIE ,  SHIMBA NOBUHISA ,  XU NINGCHUN ,  KAI YUKO ,  HARA SEIICHI ,  WATANABE KUNIHIKO ,  TAGAMI UNO ,  ONOYE HIROMI ,  SUZUKI SONOKO ,  YOKOZEKI KENZO

IPC: C12N15/09 ,  C12N9/00 ,  C12P13/00 ,  C12P21/02

Abstract: The present invention aims at providing an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. The peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II). (I) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations 1 to 68, and the mutations 239 to 290 and 324 to 377 in an amino acid sequence of SEQ ID NO:2. (II) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations L1 to L335 and M1 to M642 in an amino acid sequence of SEQ ID NO:208

公开(公告)号：BR0316927A

公开(公告)日：2005-10-18

申请号：BR0316927

申请日：2003-12-09

Applicant: AJINOMOTO KK

Inventor： SUGIYAMA MASAKAZU ,  WATANABE KUNIHIKO ,  KASHIWAGI TATSUKI ,  SUZUKI EI-ICHIRO

IPC: C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N9/10 ,  C12P17/10 ,  C12N15/09 ,  C12N5/10 ,  C12P13/04

Abstract: A D-aminotransferase can be modified so as to efficiently produce (2R, 4R) -monatin having high sweetness intensity from 4- (indol-3-ylmethyl) -4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiently producing the (2R, 4R) -monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.

公开(公告)号：BR9903646A

公开(公告)日：2000-10-17

申请号：BR9903646

申请日：1999-08-11

Applicant: AJINOMOTO KK

Inventor： TONOUCHI NOATO ,  SUGIYAMA MASAKAZU ,  YOKOZEKI KENZO

IPC: C12N15/09 ,  C12N1/21 ,  C12N15/00 ,  C12N15/64 ,  C12N15/74 ,  C12R1/01 ,  C12N15/70 ,  C12R1/185

Abstract: A shuttle vector autonomously replicable in bacteria belonging to the genera Gluconobacter and Escherichia is constructed from an endogenous plasmid of Gluconobacter oxydans IFO3171 strain, which has a size of about 5.6 kb, or a part thereof, and a plasmid autonomously replicable in bacteria belonging to the genus Escherichia or a part thereof. The present invention provides a novel plasmid useful for gene manipulation of Gluconobacter bacteria, and a shuttle vector utilizing the plasmid.

公开(公告)号：PE10232000A1

公开(公告)日：2000-10-10

申请号：PE00076399

申请日：1999-07-27

Applicant: AJINOMOTO KK

Inventor： SUGIYAMA MASAKAZU ,  SUZUKI SHUNICHI ,  YOKOZEKI KENZO ,  TONOUCHI NAOTO

IPC: C12N9/02 ,  C12N1/21 ,  C12N9/04 ,  C12N15/52 ,  C12N15/53 ,  C12P7/18

CPC classification number: C12N9/0006 ,  C12P7/18

Abstract: SE REFIERE A UNA ENZIMA XILITOL DESHIDROGENASA DE 343 O 262 AMINOACIDOS (SEC ID NO:4 O SEC ID NO:6) O DE 343 O 262 AMINOACIOS (SEC ID NO:4 O SEC ID NO:6) CON SUSTITUCION, SUPRESION, INSERCION, ADICION, INVERSION DE UNO O VARIOS AMINOACIDOS QUE PRODUCE XILITOL A PARTIR DE D-XILULOSA. TAMBIEN SE REFIERE A UN ADN QUE CODIFICA DICHA ENZIMA. TAMBIEN SE REFIERE A UN ADN QUE CONTIENE POR LO MENOS DEL NUCLEOTIDO 25 HASTA EL NUCLEOTIDO 1053 DE UNA SECUENCIA DE 1056 NUCLEOTIDOS (SEC ID NO:3) O UN ADN HIBRIDIZABLE CON UN ADN QUE TIENE UNA SECUENCIA DE 1056 NUCLEOTIDOS (SEC ID NO:3) O UNA SONDA PREPARADA A PARTIR DE LA SECUENCIA NUCLEOTIDICA EN CONDICIONES RIGUROSAS EL LAVADO SE REALIZA A 60°C Y A UNA CONCENTRACION SALINA A 1 x SSC Y 0.1% DE SDS. TAMBIEN SE REFIERE A UN ADN QUE TIENE UNA SECUENCIA NUCLEOTIDICA DEL NUCLEOTIDO 1063 HASTA EL NUCLEOTIDO 1848 DE UNA SECUENCIA DE 2774 NUCLEOTIDOS (SEC ID NO:5). TAMBIEN SE REFIERE A UN METODO PARA PRODUCIR LA DESHIDROGENASA DE XILITOL A PARTIR DE UNA CELULA A LA QUE SE LE HA INTRODUCIDO ADN QUE CODIFICA LA DESHIDROGENASA DE XILITOL. TAMBIEN SE REFIERE A UN METODO PARA PRODUCIR XILITOL A PARTIR DE D-XILULOSA

公开(公告)号：BR9814822A

公开(公告)日：2000-10-03

申请号：BR9814822

申请日：1998-10-15

Applicant: AJINOMOTO KK

Inventor： SUGIYAMA MASAKAZU ,  SUZUKI SHUNICHI ,  MIHARA YASUHIRO ,  HASHIGUCHI KENICHI ,  YOKOZEKI KENZO

IPC: C12P7/18 ,  C12R1/01 ,  C12R1/02 ,  C12R1/025 ,  C12R1/03 ,  C12R1/04 ,  C12R1/06 ,  C12R1/065 ,  C12R1/13 ,  C12R1/15 ,  C12R1/18 ,  C12R1/20 ,  C12R1/265 ,  C12R1/365 ,  C12R1/38 ,  C12R1/465 ,  C12R1/48 ,  C12R1/545 ,  C12R1/56 ,  C12R1/58

Abstract: A process for preparing xylitol, characterized by allowing a microorganism belonging to the genus Gluconobacter, Acetobacter, Achromobacter, Agrobacterium, Arthrobacter, Azotobacter, Brevibacterium, Corynebacterium, Erwinia, Flavobacterium, Micrococcus, Norcardia, Planococcus, Pseudomonas, Rhodococcus, Morganella, Actinomadura, Actinomyces, or Streptomyces and having the capability of converting D-arabitol to xylitol to act on D-arabitol and harvesting the xylitol thus produced.

公开(公告)号：AT554178T

公开(公告)日：2012-05-15

申请号：AT05787567

申请日：2005-09-29

Applicant: AJINOMOTO KK

Inventor： SUGIYAMA MASAKAZU ,  WATANABE KUNIHIKO ,  TAKEMOTO TADASHI ,  MORI KENICHI

IPC: C12P13/04 ,  C12P13/22 ,  C12R1/01 ,  C12R1/185 ,  C12R1/19

Abstract: The present invention is to provide an industrially advantageous method for producing an amino acid derivative. Provided is a method for producing an amino acid derivative including contacting a microorganism and/or an enzyme with a hydroxyimino acid represented by the following general formula (I): wherein R 1 represents an optionally substituted predetermined hydrocarbon group; R 2 represents a C1 to C3 alkyl group or a hydrogen atom; and n is 0 or 1, to produce an amino acid derivative represented by the following general formula (III): wherein R 1 and n have the same meanings as those of R 1 and n in the general formula (I), wherein the microorganism and/or the enzyme is capable of catalyzing the reaction.

公开(公告)号：CA2507225C

公开(公告)日：2012-02-07

申请号：CA2507225

申请日：2003-12-09

Applicant: AJINOMOTO KK

Inventor： SUGIYAMA MASAKAZU ,  WATANABE KUNIHIKO ,  KASHIWAGI TATSUKI ,  SUZUKI EI-ICHIRO

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/10 ,  C12P13/04 ,  C12P17/10

Abstract: A D-aminotransferase can be modified so as to efficiently produce (2R, 4R)-monatin having high sweetness intensity from 4-(indol-3ylmethyl)-4-hydroxy-2-oxoglutaic acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiency producing the (2R, 4R)-monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.

公开(公告)号：ES2357765T3

公开(公告)日：2011-04-29

申请号：ES02783795

申请日：2002-12-09

Applicant: AJINOMOTO KK

Inventor： SUGIYAMA MASAKAZU ,  WATANABE KUNIHIKO ,  FUNAKOSHI NAO ,  AMINO YUSUKE ,  KAWAHARA SHIGERU ,  TAKEMOTO TADASHI

IPC: C12P13/04 ,  C12P13/14 ,  C12P17/10

Abstract: Un procedimiento para producir un derivado de glutamato, incluyendo formas salinas del mismo, de la fórmula general (2) en la que R 1 representa un grupo fenilmetilo o un grupo 3-indolilmetilo, y R 2 representa un grupo hidroxilo, en presencia de una transaminasa que cataliza una reacción para producir el derivado de glutamato de la fórmula general (2) a partir de un ácido α-ceto sustituido de la siguiente fórmula general (1) en la que R 1 representa un grupo fenilmetilo o un grupo 3-indolilmetilo, y R 2 representa un grupo hidroxilo, que incluye una etapa de proseguir la reacción.

公开(公告)号：CA2714881A1

公开(公告)日：2004-03-04

申请号：CA2714881

申请日：2003-08-26

Applicant: AJINOMOTO KK

Inventor： SUGIYAMA MASAKAZU ,  WATANABE KUNIHIKO

IPC: C12N15/09 ,  C12N15/60 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N9/10 ,  C12N9/88 ,  C12P7/40 ,  C12P7/42 ,  C12P7/50 ,  C12P13/04 ,  C12P13/14 ,  C12P17/10 ,  C12P21/06

Abstract: 4-(Indol-3-ylmethyl),4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.

公开(公告)号：DE69912893D1

公开(公告)日：2003-12-24

申请号：DE69912893

申请日：1999-07-30

Applicant: AJINOMOTO KK

Inventor： SUGIYAMA MASAKAZU ,  TONOUCHI NAOTO ,  SUZUKI SHUNICHI ,  YOKOZEKI KENZO

IPC: C12N9/02 ,  C12N1/21 ,  C12N9/04 ,  C12N15/53 ,  C12P7/18

Abstract: Xylitol is produced by allowing xylitol dehydrogenase or cells instoduced with a DNA coding for xylitol dehydrogenase, which is a protein of the following (A) or (B) to act on D-xylulose, and collecting produced xylitol: (A) a protein which has the amino acid sequence of SEQ ID NO: 4; (B) a protein which has the amino acid sequence of SEQ ID NO: 4 including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and has xylitol dehydrogenase activity.