Novel elongase gene and method for producing multiple-unsaturated fatty acids

    公开(公告)号:AU2001239244B2

    公开(公告)日:2006-06-08

    申请号:AU2001239244

    申请日:2001-02-08

    Applicant: BASF AG

    Abstract: The invention relates to novel elongase genes with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and/or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs. Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.

    Fatty acid desaturase gene from plants

    公开(公告)号:NZ517190A

    公开(公告)日:2003-11-28

    申请号:NZ51719000

    申请日:2000-08-23

    Applicant: BASF AG

    Abstract: An isolated nucleic acid sequence encodes a polypeptide with desaturase activity. The nucleic acid selected from: a) a nucleic acid sequence with the sequence shown in SEQ ID NO:1; b) nucleic acid sequence which as a result of the degeneracy of the genetic code are derived from the nucleic acid sequence of SEQ ID NO:1 and c) derivates of the nucleic acid sequence shown SEQ IDNO: 1 which encodes polypeptides with the amino acid sequences shown in SEQ ID NO:2 and which have at least 75 % homology at the amino acid level without substantially reducing the enzymatic activity of the polypeptides. Described are and transgenic plants and vector comprising the nucleic acid sequences. The sequences can be used in a process for the preparation of triglycerides with an increased content of unsaturated fatty acids or an increased content of saturated fatty acids and the preparation of saturated fatty acids by introducing the sequence into an oil-producing organism and isolating the oil produced.

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