公开(公告)号：CA2339967C

公开(公告)日：2011-06-07

申请号：CA2339967

申请日：1999-08-04

Applicant: BIO RAD LABORATORIES

Inventor： SEGEV DAVID

IPC: C07H21/00 ,  C12N15/09 ,  C07D403/04 ,  C07D403/14 ,  C07D473/00 ,  C07D473/30 ,  C07D473/34 ,  C07H1/00 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C12Q1/68

Abstract: A compound of a general structure: D-B-M , wherein B is selected from the group consisting of derivatives of naturally occurring nitrogenous bases having a C-H group of positions 5 or 8, and derivatives of nitrogenous base-analogs having a C-H group at positions 5 or 8; D is at least one derivatizing group, including hydrogen; and M is a maleimide derivative.

公开(公告)号：CA2382631C

公开(公告)日：2010-09-28

申请号：CA2382631

申请日：2000-07-21

Applicant: BIO RAD LABORATORIES

Inventor： SEGEV DAVID

IPC: C07D473/00 ,  C12N15/09 ,  A01N43/54 ,  A01N43/90 ,  A61K31/795 ,  A61K48/00 ,  A61P31/12 ,  A61P35/00 ,  A61P43/00 ,  C07D239/00 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C08G65/34 ,  C08G65/48 ,  C12N1/08 ,  C12N5/10 ,  C12N15/63

Abstract: A compound comprising a poly(ether-thioether), poly(ether-sulfoxide) or poly(ether-sulfone) backbone bearing a plurality of ligands that are individually bound to chiral carbon atoms located within the backbone, at least one of the ligands includ-ing a moiety such as a naturally occurring nucleobase, a nucleobase binding group or a DNA interchelator; a process of synthesizing the compound, monomers to be used in this process and their synthesis process and processes for using the compound in biochem-istry and medicine.

公开(公告)号：DE69930647T2

公开(公告)日：2007-01-11

申请号：DE69930647

申请日：1999-08-04

Applicant: BIO RAD LABORATORIES

Inventor： SEGEV DAVID

IPC: C07H21/00 ,  C12N15/09 ,  C07D403/04 ,  C07D403/14 ,  C07D473/00 ,  C07D473/30 ,  C07D473/34 ,  C07H1/00 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C12Q1/68

Abstract: A compound of a general structure:wherein:B is selected from the group consisting of derivatives of naturally occurring nitrogenous bases having a C-H group at positions 5 or 8, and derivatives of nitrogenous base-analogs having a C-H group at positions 5 or 8;D is at least one derivatizing group, including hydrogen; andM is a maleimide derivative.

公开(公告)号：AU6012600A

公开(公告)日：2001-03-26

申请号：AU6012600

申请日：2000-07-21

Applicant: BIO-RAD LABORATORIES INC

Inventor： SEGEV DAVID

IPC: C12N15/09 ,  A61K31/795 ,  A61K48/00 ,  A61P31/12 ,  A61P35/00 ,  A61P43/00 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12N5/10 ,  C12Q1/68 ,  C07H19/00 ,  C07H21/02 ,  C07H21/04 ,  A01N61/00 ,  A01N43/04

Abstract: A compound comprising a poly(ether-thioether), poly(ether-sulfoxide) or poly(ether-sulfone) backbone bearing a plurality of ligands that are individually bound to chiral carbon atoms located within the backbone, at least one of the ligands including a moiety such as a naturally occurring nucleobase, a nucleobase binding group or a DNA interchelator; a process of synthesizing the compound, monomers to be used in this process and their synthesis process and processes for using the compound in biochemistry and medicine.

公开(公告)号：CA2217325A1

公开(公告)日：1996-11-07

申请号：CA2217325

申请日：1996-04-30

Applicant: BIO RAD LABORATORIES

Inventor： SEGEV DAVID

IPC: C12Q1/68 ,  C07H1/00 ,  C07H21/00

Abstract: The invention is directed towards a method of amplifying target nucleic acids by using two oligonucleotide probe complement pairs. Each member of the probe pair contains a chemical functionality group which permits linkage of the probes when the functionality groups are adjacent to one another following hybridization of the probe pairs to the template. One probe in each pair is composed of two regions, a first region which hybridizes to the target and which contains the chemical functionality group and a second, protecting region which prevents target independent joining as shown in the figure. The other probe in the pair contains the corresponding chemical functionality group. Upon joining of a first probe pair, an amplification can proceed in which the newly joined first probe pair can serve as a template for the second, complementary probe pair, which can in turn serve as a template for unjoined first probe pairs. This cyclic amplification is sensitive enough for a discriminative amplification of sequences which differ by merely a point mutation and therefore is suitable for point mutation detection and genotype determination as well as for the determination of the presence or absence of a specific nucleic acid in a sample.

公开(公告)号：EP0828856A4

公开(公告)日：2001-11-21

申请号：EP96916435

申请日：1996-04-30

Applicant: BIO RAD LABORATORIES

Inventor： SEGEV DAVID

IPC: C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2533/107 ,  C12Q2523/101

Abstract: The invention is directed towards a method of amplifying target nucleic acids by using two oligonucleotide probe complement pairs. Each member of the probe pair contains a chemical functionality group which permits linkage of the probes when the functionality groups are adjacent to one another following hybridization of the probe pairs to the template. One probe in each pair is composed of two regions, a first region which hybridizes to the target and which contains the chemical functionality group and a second, protecting region which prevents target independent joining as shown in the figure. The other probe in the pair contains the corresponding chemical functionality group. Upon joining of a first probe pair, an amplification can proceed in which the newly joined first probe pair can serve as a template for the second, complementary probe pair, which can in turn serve as a template for unjoined first probe pairs. This cyclic amplification is sensitive enough for a discriminative amplification of sequences which differ by merely a point mutation and therefore is suitable for point mutation detection and genotype determination as well as for the determination of the presence or absence of a specific nucleic acid in a sample.

公开(公告)号：EP1208234A4

公开(公告)日：2009-12-30

申请号：EP00946256

申请日：2000-07-21

Applicant: BIO RAD LABORATORIES

Inventor： SEGEV DAVID

IPC: C12N15/09 ,  A61K31/795 ,  A61K48/00 ,  A61P31/12 ,  A61P35/00 ,  A61P43/00 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12N5/10 ,  C12Q1/68 ,  A01N43/04 ,  A01N61/00 ,  C07H19/00 ,  C07H21/02 ,  C07H21/04

CPC classification number: C07H21/00 ,  C07H19/10 ,  C07H19/20

公开(公告)号：EP1363640A4

公开(公告)日：2006-10-25

申请号：EP02711178

申请日：2002-01-29

Applicant: BIO RAD LABORATORIES

Inventor： SEGEV DAVID

IPC: C12N15/09 ,  A61K31/439 ,  A61K31/506 ,  A61K31/513 ,  A61K31/52 ,  A61K31/675 ,  A61K48/00 ,  A61P1/04 ,  A61P1/18 ,  A61P7/00 ,  A61P9/00 ,  A61P9/10 ,  A61P11/00 ,  A61P11/06 ,  A61P13/12 ,  A61P17/00 ,  A61P17/06 ,  A61P17/12 ,  A61P19/02 ,  A61P27/02 ,  A61P29/00 ,  A61P31/10 ,  A61P31/12 ,  A61P31/16 ,  A61P31/18 ,  A61P31/22 ,  A61P35/00 ,  A61P37/04 ,  C07D239/52 ,  C07D498/18 ,  C07H21/04 ,  C12Q1/68 ,  A61K31/535 ,  A61K31/045 ,  A61K31/22 ,  A61K31/505 ,  C07C31/18 ,  C07C31/24 ,  C07C67/02 ,  C07C69/74 ,  C07D239/02 ,  C07D265/34 ,  C07D267/22 ,  C07D403/00

CPC classification number: C12Q1/6839 ,  C07B2200/11 ,  C12Q1/6832 ,  C12Q2525/117 ,  C12Q2525/113

公开(公告)号：EP1102782A4

公开(公告)日：2002-01-30

申请号：EP99940867

申请日：1999-08-04

Applicant: BIO RAD LABORATORIES

Inventor： SEGEV DAVID

IPC: C12N15/09 ,  C07D403/04 ,  C07D403/14 ,  C07D473/00 ,  C07D473/30 ,  C07D473/34 ,  C07H1/00 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C12Q1/68 ,  C07H21/00

CPC classification number: C07D473/00 ,  C07D403/04 ,  C07D473/30 ,  C07D473/34 ,  C07H19/10 ,  C07H19/20

Abstract: A compound of a general structure: D-B-M , wherein B is selected from the group consisting of derivatives of naturally occurring nitrogenous bases having a C-H group of positions 5 or 8, and derivatives of nitrogenous base-analogs having a C-H group at positions 5 or 8; D is at least one derivatizing group, including hydrogen; and M is a maleimide derivative.