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    POLYETHER NUCLEIC ACID
    1.
    发明专利
    POLYETHER NUCLEIC ACID 失效

    公开(公告)号：JPH10257888A

    公开(公告)日：1998-09-29

    申请号：JP31286697

    申请日：1997-10-30

    Applicant: BIO RAD LABORATORIES

    Inventor： SEGEV DAVID

    IPC: C12N15/09 ,  A61K31/765 ,  A61K48/00 ,  A61P31/04 ,  A61P31/12 ,  A61P35/00 ,  C07H21/00 ,  C07K1/00 ,  C07K19/00 ,  C08G65/32 ,  C12Q1/68

    Abstract: PROBLEM TO BE SOLVED: To obtain the subject new nucleic acid, containing a polyether skeleton carrying a plurality of ligands individually bound to a chiral carbon atom in the skeleton such as a natural nucleobase, its binding group and a DNA inserting factor and useful as a pseudonucleotide, etc., useful in biochemistry and medicine. SOLUTION: This new polyether nucleic acid comprises a polyether skeleton, having the formula [(n) is an integer of >1; B1 to Bn are each a chemical functional group and at least one of B1 to Bn is a natural nucleobase, a nucleobase binding group or a DNA inserting factor; Y1 to Yn are each the first linker group; X1 to Xn are each the second linker group; C1 to Cn are each a chiral carbon atom; K and I are each the first and the second exoconjugant] and carrying a plurality of ligands individually bound to the chiral carbon atom located in the skeleton and at least one of the ligands contains components such as the natural nucleobase, nucleobase binding group and DNA inserting factor. The polyether nucleic acid is useful as an antisense oligonucleotide analog, etc., capable of controlling the gene expression in the fields of biochemistry and medicine.

    NUCLEIC ACID DETECTION AND AMPLIFICATION BY CHEMICAL LINKAGEOF OLIGONUCLEOTIDES
    8.
    发明专利
    NUCLEIC ACID DETECTION AND AMPLIFICATION BY CHEMICAL LINKAGEOF OLIGONUCLEOTIDES 未知

    公开(公告)号：CA2217325C

    公开(公告)日：2007-11-27

    申请号：CA2217325

    申请日：1996-04-30

    Applicant: BIO RAD LABORATORIES

    Inventor： SEGEV DAVID

    IPC: C12Q1/68 ,  C07H1/00 ,  C07H21/00

    Abstract: The invention is directed towards a method of amplifying target nucleic acid s by using two oligonucleotide probe complement pairs. Each member of the probe pair contains a chemical functionality group which permits linkage of the probes when the functionality groups are adjacent to one another following hybridization of the probe pairs to th e template. One probe in each pair is composed of two regions, a first region which hybridizes to the target and which contains the chemica l functionality group and a second, protecting region which prevents target independent joining as shown in the figure. The other probe in the pair contains the corresponding chemical functionality group. Upon joining of a first probe pair, an amplification can proceed in which the newly joined first probe pair can serve as a template for the second, complementary probe pair, which can in turn serve as a template for unjoined first probe pairs. This cyclic amplification is sensitive enough for a discriminative amplification of sequences which diffe r by merely a point mutation and therefore is suitable for point mutation detection and genotype determination as well as for the determinati on of the presence or absence of a specific nucleic acid in a sample.

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