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    METHODS FOR INCREASING THE INFECTIVITY OF VIRUSES
    23.
    发明公开
    METHODS FOR INCREASING THE INFECTIVITY OF VIRUSES 有权
    提高病毒感染力的方法

    公开(公告)号：EP2807252A4

    公开(公告)日：2015-10-14

    申请号：EP13740994

    申请日：2013-01-25

    Applicant: LIFE TECHNOLOGIES CORP ,  UNIV JOHNS HOPKINS

    Inventor： AGNEW BRIAN ,  GRAHAM DAVID

    IPC: C12N7/00

    CPC classification number: C12N7/00 ,  A61K39/00 ,  A61K48/00 ,  C12N2740/16034 ,  C12N2740/16045 ,  C12N2740/16051 ,  C12N2810/10

    Abstract: Methods of using viruses labeled with alkyne-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to increase the infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an alkyne-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The viruses labeled with alkyne-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.

    Abstract translation: 使用用炔烃修饰的生物分子（例如脂肪酸，碳水化合物和脂质）标记的病毒的方法来治疗植物，昆虫或感染病毒的动物或增加病毒如人类免疫缺陷病毒的感染性， 被提供。 还提供了用炔修饰的生物分子如脂肪酸，碳水化合物或类异戊二烯脂质来标记病毒（例如人类免疫缺陷病毒）的方法。 用炔烃修饰的生物分子标记的病毒可以与药学上可接受的赋形剂组合以产生药物组合物，任选地含有另一种抗病毒剂和/或递送剂，例如脂质体。

    METHODS FOR INCREASING THE INFECTIVITY OF VIRUSES
    24.
    发明公开
    METHODS FOR INCREASING THE INFECTIVITY OF VIRUSES 有权
    法提高病毒的感染

    公开(公告)号：EP2807251A4

    公开(公告)日：2015-08-12

    申请号：EP13740701

    申请日：2013-01-25

    Applicant: LIFE TECHNOLOGIES CORP

    Inventor： AGNEW BRIAN

    IPC: C12N7/00

    CPC classification number: C12N7/00 ,  A61K39/00 ,  C12N2740/16045 ,  C12N2740/16052 ,  C12N2810/10

    Abstract: Methods of using viruses labeled with alkyne-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to increase the infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an alkyne-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The viruses labeled with alkyne-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.

    METHODS AND COMPOSITIONS FOR LABELING NUCLEIC ACIDS
    28.
    发明公开
    METHODS AND COMPOSITIONS FOR LABELING NUCLEIC ACIDS 审中-公开
    VERFAHREN UND ZUSAMMENSETZUNGEN ZUR MARKIERUNG VONNUKLEINSÄUREN

    公开(公告)号：EP1937849A4

    公开(公告)日：2013-03-13

    申请号：EP06836555

    申请日：2006-10-27

    Applicant: HARVARD COLLEGE ,  LIFE TECHNOLOGIES CORP

    Inventor： SALIC ADRIAN ,  MITCHISON TIMOTHY J ,  GEE KYLE R ,  AGNEW BRIAN

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N33/5011 ,  G01N33/582 ,  C12Q2525/101 ,  C12Q2563/107

    Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.

    Abstract translation: 本发明涉及在体外和体内标记核酸聚合物的方法。 提供了包括掺入核酸聚合物的核苷酸类似物与附着在标记上的试剂之间的[3 + 2]环加成的某些方法。 提供了包括掺入核酸聚合物的核苷酸类似物与包含连接到标记上的取代的三芳基膦的试剂之间的施陶丁格连接的其它方法。 这样的方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。 还提供了测量细胞增殖的方法。 在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。 本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。

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