公开(公告)号：WO2012112609A3

公开(公告)日：2012-10-18

申请号：PCT/US2012025123

申请日：2012-02-14

Applicant: LIFE TECHNOLOGIES CORP ,  LAKSHMIPATHY UMA ,  SINGH UPINDER ,  GRECIAN SCOTT ,  QUINTANILLA RENE ,  GEE KYLE ,  RAO MAHENDRA

Inventor： LAKSHMIPATHY UMA ,  SINGH UPINDER ,  GRECIAN SCOTT ,  QUINTANILLA RENE ,  GEE KYLE ,  RAO MAHENDRA

IPC: G01N33/58 ,  G01N33/50

CPC classification number: G01N33/5005 ,  C09B11/24 ,  C09B19/00 ,  C09B21/00 ,  C09B57/00 ,  C09B57/02 ,  C12Q1/42 ,  C12Y301/03001 ,  G01N1/30 ,  G01N33/5073 ,  G01N33/581 ,  G01N33/582 ,  G01N33/586 ,  G01N2001/302 ,  G01N2333/916

Abstract: The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.

Abstract translation: 本发明涉及用于染色活的干细胞的新的底物和方法。 染色可用于在体细胞重编程过程中鉴定诱导的多能干细胞集落。

公开(公告)号：DK1991273T4

公开(公告)日：2022-02-07

申请号：DK07825657

申请日：2007-02-12

Applicant: LIFE TECHNOLOGIES CORP

Inventor： AGNEW BRIAN ,  GEE KYLE ,  NYBERG TAMARA

IPC: C12P21/00 ,  C07K17/00

公开(公告)号：CL2021000340A1

公开(公告)日：2021-07-02

申请号：CL2021000340

申请日：2021-02-09

Applicant: LIFE TECHNOLOGIES CORP

Inventor： EVANS BRIAN ,  YANG JONGTAE ,  WANG XIN ,  HU YI-ZHEN ,  GEE KYLE ,  CHEN AIMEI ,  BENSON SCOTT ,  RUKAVISHNIKOV ALEKSEY

IPC: C09B11/14 ,  C09B69/00 ,  C12Q1/6869 ,  G01N33/58

Abstract: Los compuestos de rodamina sustituidos con silicio se describen en la presente. En la presente se describen también tintes de SiR que comprenden al menos un grupo vinilo unido al átomo de Si (posición 10) del tinte de SiR. También se proporcionan derivados, versiones funcionalizadas, conjugados, kits, métodos sintéticos relacionados y usos de compuestos de SiR. Los tintes de silicio-rodamina (SiR) pueden proporcionar una fluorescencia brillante en longitudes de onda de rojo lejano y mostrar buena fotoestabilidad. Los compuestos descritos en la presente pueden ser útiles para el marcado fluorescente y la detección de muestras biológicas.

公开(公告)号：CA3107758A1

公开(公告)日：2020-02-13

申请号：CA3107758

申请日：2019-08-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YANG JONGTAE ,  WANG XIN ,  HU YI-ZHEN ,  GEE KYLE ,  CHEN AIMEI ,  BENSON SCOTT ,  EVANS BRIAN ,  RUKAVISHNIKOV ALEKSEY

IPC: C09B69/00 ,  C09B11/14 ,  C12Q1/6869 ,  G01N33/58

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are Si R dyes comprising at least one vinyl groupattached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of Si R compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples. The compounds have the general structure (I) below.

公开(公告)号：AU2019319901B2

公开(公告)日：2022-07-07

申请号：AU2019319901

申请日：2019-08-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YANG JONGTAE ,  WANG XIN ,  HU YI-ZHEN ,  GEE KYLE ,  CHEN AIMEI ,  BENSON SCOTT ,  EVANS BRIAN ,  RUKAVISHNIKOV ALEKSEY

IPC: C09B69/00 ,  C09B11/14 ,  C12Q1/6869 ,  G01N33/58

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are Si R dyes comprising at least one vinyl groupattached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of Si R compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples. The compounds have the general structure (I) below.

公开(公告)号：AU2019319901A1

公开(公告)日：2021-02-18

申请号：AU2019319901

申请日：2019-08-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YANG JONGTAE ,  WANG XIN ,  HU YI-ZHEN ,  GEE KYLE ,  CHEN AIMEI ,  BENSON SCOTT ,  EVANS BRIAN ,  RUKAVISHNIKOV ALEKSEY

IPC: C09B69/00 ,  C09B11/14 ,  C12Q1/6869 ,  G01N33/58

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are Si R dyes comprising at least one vinyl groupattached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of Si R compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples. The compounds have the general structure (I) below.

公开(公告)号：WO2016090076A3

公开(公告)日：2016-08-11

申请号：PCT/US2015063593

申请日：2015-12-03

Applicant: LIFE TECHNOLOGIES CORP

Inventor： ZHOU WENJUN ,  GEE KYLE ,  TENNICO YOLANDA ,  SLADE PETER ,  KANG HEE CHOL ,  KHAN SHAHEER ,  EVANS BRIAN ,  STRAY JAMES

IPC: C07D261/00 ,  G01N33/58

CPC classification number: C07C309/47 ,  C07C309/51 ,  C07C2603/50 ,  C07D311/84 ,  G01N33/582 ,  G01N2400/10

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

Abstract translation: 本公开内容涉及含有肼基取代基和任选地一个或多个带负电基团（例如磺酸根，磷酸根，膦酸根和/或羧酸根基团）和含有氨氧基取代基的染料化合物的染料化合物。 这些化合物可用于检测含有醛和酮基的分析物，包括例如聚糖。

公开(公告)号：WO2010078333A3

公开(公告)日：2010-10-28

申请号：PCT/US2009069696

申请日：2009-12-29

Applicant: LIFE TECHNOLOGIES CORP ,  GEE KYLE ,  MARTIN VLADIMIR

Inventor： GEE KYLE ,  MARTIN VLADIMIR

IPC: C07D263/58 ,  C07D263/56

CPC classification number: G01N21/75 ,  C07D263/56 ,  C07D263/57 ,  C07D401/04 ,  C07D413/04 ,  C07D417/04 ,  C07D471/06 ,  C07D491/16 ,  C09B57/00 ,  C09B57/02 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1029 ,  C09K2211/1033 ,  C09K2211/1037 ,  C09K2211/1048 ,  C09K2211/1088 ,  G01N33/582 ,  G01N33/84

Abstract: Disclosed are benzoxazole-based compounds, kits, and methods of producing and using the described compounds in fluorescence-based detection of analytes (e.g., metal ions). Also disclosed are uses of benzoxazole-based compounds as ratiometric metal ion indicators.

Abstract translation: 公开了基于苯并恶唑的化合物，试剂盒以及在分析物（例如金属离子）的基于荧光的检测中生产和使用所述化合物的方法。 还公开了苯并恶唑基化合物作为比率金属离子指示剂的用途。

公开(公告)号：WO2014066733A3

公开(公告)日：2014-06-19

申请号：PCT/US2013066765

申请日：2013-10-25

Applicant: LIFE TECHNOLOGIES CORP

Inventor： AGNEW BRIAN ,  AGGELER ROBERT ,  KANG HEE CHOL ,  CHEN AIMEI ,  GEE KYLE

IPC: G01N33/50

CPC classification number: A61K51/10 ,  C07K14/435 ,  C07K16/18 ,  C12P21/005 ,  C12P21/06 ,  G01N33/534 ,  G01N33/582 ,  G01N33/6854

Abstract: Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.

Abstract translation: 本文提供了用于糖蛋白的位点特异性标记的方法，组合物和试剂盒，其包括酶介导的包含化学处理和环加成化学的修饰的糖的组合与包含反应性基团的标记分子，金属离子螯合剂， 和/或荧光团。

公开(公告)号：SG11202101282QA

公开(公告)日：2021-03-30

申请号：SG11202101282Q

申请日：2019-08-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YANG JONGTAE ,  WANG XIN ,  HU YI-ZHEN ,  GEE KYLE ,  CHEN AIMEI ,  BENSON SCOTT ,  EVANS BRIAN ,  RUKAVISHNIKOV ALEKSEY

IPC: C09B69/00 ,  C09B11/14 ,  C12Q1/6869 ,  G01N33/58

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are SiR dyes comprising at least one vinyl group attached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of SiR compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples.