公开(公告)号：US20150133649A1

公开(公告)日：2015-05-14

申请号：US14536429

申请日：2014-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian REED ,  James A. Ball

IPC: B01D17/04 ,  C07H21/00 ,  C07H1/06

CPC classification number: B01D17/047 ,  B01D17/04 ,  B01D17/041 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2563/159

Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

Abstract translation: 从乳液中回收珠粒载体的方法包括将表面活性剂水溶液供给到离心管中; 在离心管中的表面活性剂溶液上提供疏水性液体，其中表面活性剂水溶液的体积与疏水性液体的体积之比不大于0.5; 并且在离心时将乳液施加在疏水性液体上，所述乳液包含分散的水相，包括珠粒支持体，乳液破碎和分散相的材料优先分配至表面活性剂溶液。

公开(公告)号：US11229859B2

公开(公告)日：2022-01-25

申请号：US16786913

申请日：2020-02-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian Reed ,  James A. Ball

IPC: C07H1/06 ,  B01D17/04 ,  C07H21/00 ,  C12Q1/6806

Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

公开(公告)号：US10809225B2

公开(公告)日：2020-10-20

申请号：US14789795

申请日：2015-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ronald L. Cicero ,  Marc Glazer ,  Yufang Wang ,  Christina E. Inman ,  Jeremy Gray ,  Joseph Koscinski ,  James A. Ball ,  Phil Waggoner ,  Alexander Mastroianni

IPC: G01N27/414

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

公开(公告)号：US10724086B2

公开(公告)日：2020-07-28

申请号：US15061273

申请日：2016-03-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Phil Waggoner ,  James A. Ball ,  Wolfgang Hinz ,  Michael L. Minto ,  Scott Parker ,  David M. Cox ,  Alexander Mastroianni ,  Jeremy Gray ,  Marc Glazer ,  Kimberly Gorrell

IPC: C12Q1/6869 ,  G01N27/414

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

公开(公告)号：US10527580B2

公开(公告)日：2020-01-07

申请号：US16045682

申请日：2018-07-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James A. Ball ,  Brian Reed

IPC: C11D7/34 ,  G01N27/414 ,  B08B3/08 ,  C11D7/50 ,  B08B9/08 ,  C11D3/16 ,  C11D3/34 ,  C11D3/43

Abstract: A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. A method of treating the sensor array includes exposing at least the sensor pad to a wash solution including sulfonic acid and an organic solvent and rinsing the wash solution from the sensor pad.

公开(公告)号：US10240193B2

公开(公告)日：2019-03-26

申请号：US15891485

申请日：2018-02-08

Applicant: Life Technologies Corporation

Inventor： Jonathan Schultz ,  Todd Roswech ,  Jon A. Hoshizaki ,  Albert L. Carrillo ,  James A. Ball

IPC: C12Q1/68 ,  B01L3/00 ,  G01N27/414 ,  G01N35/10 ,  B01F11/00 ,  B01F1/00 ,  B01F5/06 ,  C12Q1/6869 ,  C12Q1/6806

Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

公开(公告)号：US20190070640A1

公开(公告)日：2019-03-07

申请号：US16182429

申请日：2018-11-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James A. Ball ,  Phil Waggoner ,  Scott Parker

IPC: B08B3/08 ,  C11D11/00 ,  B08B9/08 ,  C11D1/22 ,  C11D3/16 ,  C11D3/43 ,  H01L21/02 ,  G01N27/414 ,  C11D3/34 ,  B05D5/12 ,  C11D7/34 ,  C11D7/50 ,  B05D3/00

CPC classification number: B08B3/08 ,  B05D3/00 ,  B05D5/12 ,  B08B9/08 ,  C11D1/22 ,  C11D3/162 ,  C11D3/3418 ,  C11D3/43 ,  C11D7/34 ,  C11D7/5013 ,  C11D7/5022 ,  C11D11/0047 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4148 ,  H01L21/02057

Abstract: A method of treating a sensor array including a plurality of sensors and an isolation structure, where a sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array and the isolation structure is disposed between the sensor pad and sensor pads of other sensors of the plurality of sensors, comprises exposing the sensor pad and the isolation structure to a non-aqueous organo-silicon solution including an organo-silicon compound and a first non-aqueous carrier; applying an acid solution including an organic acid and a second non-aqueous carrier to the sensor pad; and rinsing the acid solution from the sensor pad and the isolation structure.

公开(公告)号：US10221451B2

公开(公告)日：2019-03-05

申请号：US14754231

申请日：2015-06-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  David Light ,  Todd Rearick ,  James A. Ball

IPC: C12Q1/6874 ,  G01N27/414

Abstract: The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.

公开(公告)号：US10137480B2

公开(公告)日：2018-11-27

申请号：US15387591

申请日：2016-12-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James A. Ball ,  Phil Waggoner ,  Scott Parker

IPC: B08B7/00 ,  B08B3/08 ,  B08B9/08 ,  C11D3/16 ,  G01N27/414 ,  C11D3/34 ,  C11D3/43 ,  C11D1/22 ,  C11D11/00 ,  H01L21/02 ,  C11D7/50 ,  C11D7/34 ,  B05D3/00 ,  B05D5/12

Abstract: A method of treating a sensor array including a plurality of sensors and an isolation structure, where a sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array and the isolation structure is disposed between the sensor pad and sensor pads of other sensors of the plurality of sensors, comprises exposing the sensor pad and the isolation structure to a non-aqueous organo-silicon solution including an organo-silicon compound and a first non-aqueous carrier; applying an acid solution including an organic acid and a second non-aqueous carrier to the sensor pad; and rinsing the acid solution from the sensor pad and the isolation structure.

公开(公告)号：US09555451B2

公开(公告)日：2017-01-31

申请号：US15079532

申请日：2016-03-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James A. Ball ,  Phil Waggoner ,  Scott Parker

IPC: B08B7/00 ,  B08B3/08 ,  B08B9/08 ,  C11D3/16 ,  C11D3/34 ,  C11D3/43 ,  G01N27/414 ,  C11D7/50 ,  C11D7/34

CPC classification number: B08B3/08 ,  B05D3/00 ,  B05D5/12 ,  B08B9/08 ,  C11D1/22 ,  C11D3/162 ,  C11D3/3418 ,  C11D3/43 ,  C11D7/34 ,  C11D7/5013 ,  C11D7/5022 ,  C11D11/0047 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4148 ,  H01L21/02057

Abstract: A method of treating a sensor array including a plurality of sensors and an isolation structure, where a sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array and the isolation structure is disposed between the sensor pad and sensor pads of other sensors of the plurality of sensors, comprises exposing the sensor pad and the isolation structure to a non-aqueous organo-silicon solution including an organo-silicon compound and a first non-aqueous carrier; applying an acid solution including an organic acid and a second non-aqueous carrier to the sensor pad; and rinsing the acid solution from the sensor pad and the isolation structure.

Abstract translation: 一种处理包括多个传感器和隔离结构的传感器阵列的方法，其中所述多个传感器中的传感器具有在所述传感器阵列的表面处暴露的传感器垫，并且所述隔离结构设置在所述传感器垫和传感器垫之间 所述多个传感器中的其它传感器包括将所述传感器垫和所述隔离结构暴露于包括有机硅化合物和第一非水载体的非水有机硅溶液; 将包含有机酸和第二非水性载体的酸溶液施加到传感器垫; 以及从传感器垫和隔离结构冲洗酸溶液。