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    Sequencing device
    21.
    发明授权
    Sequencing device 有权

    公开(公告)号：US10894982B2

    公开(公告)日：2021-01-19

    申请号：US16289508

    申请日：2019-02-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz ,  Todd Roswech ,  Jon A. Hoshizaki ,  Albert L. Carrillo ,  James A. Ball

    IPC: C12Q1/6869 ,  C12Q1/6806 ,  B01L3/00 ,  G01N27/414 ,  G01N35/10 ,  B01F5/06 ,  B01F11/00 ,  B01F1/00

    Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

    SYSTEM AND METHODS FOR MAKING AND PROCESSING EMULSIONS
    26.
    发明申请
    SYSTEM AND METHODS FOR MAKING AND PROCESSING EMULSIONS 有权
    制备和处理乳液的系统和方法

    公开(公告)号：US20150224457A1

    公开(公告)日：2015-08-13

    申请号：US14696642

    申请日：2015-04-27

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan SCHULTZ ,  John Nobile ,  Brian Reed ,  Prasanna Krishnan Thwar ,  Todd Roswech

    IPC: B01F5/06

    CPC classification number: B01F5/0602 ,  B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F2215/0037 ,  B01L3/502784 ,  B04B5/0414 ,  B04B7/02

    Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

    Abstract translation: 提供了一种自动化的在线模板珠粒制备系统，包括膜基乳液生成子系统，乳液PCR（ePCR）热循环板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式向逆循环子系统提供连续的反相乳液流。 ePCR子系统可以连续热循环通过的反相乳液，并包括静态温度区和可消耗的热循环板。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。

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