-
公开(公告)号:US20190264274A1
公开(公告)日:2019-08-29
申请号:US16289508
申请日:2019-02-28
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Jonathan Schultz , Todd Roswech , Jon A. Hoshizaki , Albert L. Carrillo , James A, Ball
IPC: C12Q1/6869 , B01F5/06 , B01F11/00 , B01L3/00 , G01N35/10 , C12Q1/6806 , B01F1/00 , G01N27/414
Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.
-
公开(公告)号:US20180221865A1
公开(公告)日:2018-08-09
申请号:US15945738
申请日:2018-04-04
Applicant: Life Technologies Corporation
Inventor: Jonathan Schultz , Todd Roswech
Abstract: An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.
-
公开(公告)号:US09458485B2
公开(公告)日:2016-10-04
申请号:US14799259
申请日:2015-07-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Jonathan Schultz , John Nobile , Brian Reed , Prasanna Krishnan Thwar , Todd Roswech , John Andrew Sheridan
IPC: C12P19/34 , C12M1/26 , B01F15/00 , B04B11/02 , B01F5/04 , B01F13/00 , B01L3/00 , B01L7/00 , B04B5/04 , B04B7/02 , B01F3/08 , C12Q1/68
CPC classification number: B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F15/00 , B01F2215/0037 , B01L3/5021 , B01L3/502784 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/06 , B01L2300/18 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0409 , B04B5/0414 , B04B7/02 , B04B11/02 , B04B2007/025 , C12M33/10 , C12P19/34 , C12Q1/686 , C12Q2523/32 , C12Q2563/159 , C12Q2565/629
Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
-
公开(公告)号:US11987842B2
公开(公告)日:2024-05-21
申请号:US17146376
申请日:2021-01-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Jonathan Schultz , Todd Roswech , Jon A. Hoshizaki , Albert L. Carrillo , James A Ball
IPC: C12Q1/6869 , B01F1/00 , B01F11/00 , B01F21/20 , B01F25/451 , B01F31/31 , B01F31/65 , B01L3/00 , C12Q1/6806 , G01N27/414 , G01N35/10
CPC classification number: C12Q1/6869 , B01F21/22 , B01F25/451 , B01F31/311 , B01F31/65 , B01L3/502 , B01L3/527 , C12Q1/6806 , G01N27/4145 , G01N35/1002 , G01N35/1095 , B01L3/505 , B01L3/563 , B01L2200/04 , B01L2200/16 , B01L2300/022 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0683 , Y10T436/11 , Y10T436/143333
Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.
-
公开(公告)号:US20230044160A1
公开(公告)日:2023-02-09
申请号:US17970202
申请日:2022-10-20
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Jonathan Schultz , Todd Roswech
Abstract: An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.
-
公开(公告)号:US10934155B2
公开(公告)日:2021-03-02
申请号:US16587678
申请日:2019-09-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Todd Roswech , Jonathan Schultz , Chun Ho
Abstract: A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.
-
公开(公告)号:US20170066642A1
公开(公告)日:2017-03-09
申请号:US15259245
申请日:2016-09-08
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Todd Roswech , Jonathan SCHULTZ , Chun HO
CPC classification number: B67D7/0266 , B01L3/502 , B01L3/523 , B01L3/563 , B01L2200/026 , B01L2200/0684 , B01L2200/0689 , B01L2300/043 , B01L2400/0487 , B67D7/0288 , B67D7/76 , B67D2210/0001 , G01N35/1002
Abstract: A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.
Abstract translation: 流体互连包括包括液体端口,气体端口和支架的第一接口; 第二接口,包括液体端口,气体端口和摆动杆以接合支架,容器的重量附接到第一或第二接口中的一个,以驱动第一接口的液体端口与液体端口连接 的第二接口和第一接口的气体端口连接到第二接口的气体端口。
-
8.
公开(公告)号:US11588381B2
公开(公告)日:2023-02-21
申请号:US17011234
申请日:2020-09-03
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Todd Roswech
IPC: B01L3/00 , H02K11/20 , H02K5/10 , B04B5/04 , C12Q1/6874 , F16K99/00 , B04B11/04 , G01N35/00 , G01N35/04
Abstract: A rotor assembly includes a rotor plate to rotate around a first axis, a bucket attached to the rotor plate and to rotate around a second axis, and a stop plate to rotate around the first axis between an open position and a closed position. When in the closed position, the stop plate engages the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly. The rotor assembly further includes a housing for a sensor array component, the housing disposed in the bucket and including a solution inlet, a solution outlet, a transfer basin, a solution retainer disposed between the solution outlet and the transfer basin, and a collection reservoir in fluid communication with the transfer basin. The solution inlet and the solution outlet to engage ports of a flow cell of a sensor array.
-
公开(公告)号:US11478786B2
公开(公告)日:2022-10-25
申请号:US17004804
申请日:2020-08-27
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Jonathan Schultz , Todd Roswech
Abstract: An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.
-
公开(公告)号:US11214478B2
公开(公告)日:2022-01-04
申请号:US17165196
申请日:2021-02-02
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Todd Roswech , Jonathan Schultz , Chun Ho
Abstract: A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.
-
-
-
-
-
-
-
-
-