公开(公告)号：JP2011122957A

公开(公告)日：2011-06-23

申请号：JP2009281331

申请日：2009-12-11

Applicant: Tosoh Corp ,  東ソー株式会社

Inventor： MATSUBA TAKAO

IPC: G01N33/536 ,  G01N33/53 ,  G01N33/543

Abstract: PROBLEM TO BE SOLVED: To provide a method of detecting protein providing sensitivity higher than that provided by an ABC method (avidin-biotin complex method) and not affected by impurities in a specimen which have been seen as a problem in the ABC method.
SOLUTION: In this method of detecting protein, a substance for recognizing protein is labeled with estradiol and then is detected by a substance for recognizing the estradiol. A protein detection reagent is provided containing the substance labeled with the estradiol for recognizing protein and the substance for recognizing the estradiol.
COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 待解决的问题：提供一种检测蛋白质提供灵敏度高于ABC法（抗生物素蛋白 - 生物素复合物法）提供的灵敏度的方法，并且不受ABC中的问题的样本中的杂质的影响 方法。 解决方案：在这种检测蛋白质的方法中，用雌二醇标记识别蛋白质的物质，然后用​​识别雌二醇的物质检测。 提供了含有用雌二醇标记的物质识别蛋白质的蛋白质检测试剂和用于识别雌二醇的物质。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2002345474A

公开(公告)日：2002-12-03

申请号：JP2001155466

申请日：2001-05-24

Applicant: TOSOH CORP

Inventor： MATSUBA TAKAO ,  ISHIGURO NORIHIKO

IPC: G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/566 ,  G01N33/58

Abstract: PROBLEM TO BE SOLVED: To provide a simple method for measurement with a high sensitivity by which information indicating the approach of a labeled substance to a substance as an object of measurement can be amplified to raise an S/N ratio and the labeled substance is readily bound. SOLUTION: This method for measurement comprises bringing (a) a sample into contact with (b) a substance specifically binding to the substance as the object of measurement and bound to the labeled nucleic acid and (c) another substance specifically binding to the substance as the object of measurement and bound to the labeled nucleic acid, forming a conjugate, ligating the labeled nucleic acids of (b) and (c), amplifying a part containing at least the ligated part of the ligated nucleic acids or causing the synthesis of a nucleic acid indicating that the labeled nucleic acids are ligated, detecting the amplified nucleic acid and relating the obtained results to the presence or the amount of the present substance as the object of measurement.

公开(公告)号：JPH11106397A

公开(公告)日：1999-04-20

申请号：JP27001797

申请日：1997-10-02

Applicant: EIKEN CHEMICAL ,  TOSOH CORP

Inventor： FUJIMURA HIDEAKI ,  MATSUBA TAKAO ,  YASUKAWA KIYOSHI

IPC: G01N33/53 ,  A61K38/00 ,  A61P5/12 ,  C07K1/14 ,  C07K14/72 ,  C07K16/28 ,  C12N5/10 ,  C12N15/09 ,  C12P21/02

Abstract: PROBLEM TO BE SOLVED: To solubilize the subject substance for measuring TSHR autoantibody, etc., by bringing a gene recombination human thyroid-stimulating hormone receptor(TSHR) into contact with a nonionic surfactant of a solubilizer in an aqueous medium. SOLUTION: A gene encoding a human thyroid-stimulating hormone receptor, its derivative or its variant is incorporated into a vector capable of expressing the gene in a host, the gene is expressed in the host, a gene product encoded by the gene is produced in the cell membrane of the host, a cell membrane fraction is collected from the host cell to give a gene recombination human thyroid-stimulating hormone receptor. Then the human thyroid-stimulating hormone receptor is brought into contact with a nonionic surfactant as a solubilizer in an aqueous medium to solubilize the recombinant human thyroid-stimulating hormone receptor useful for measuring an anti-human TSHR autoantibody suitable for diagnosing thyropathy such as Graves' disease, etc., observing treatment process, etc.

公开(公告)号：JPH06145196A

公开(公告)日：1994-05-24

申请号：JP29968092

申请日：1992-11-10

Applicant: TOSOH CORP

Inventor： HORI HITOSHI ,  MATSUBA TAKAO ,  YAMADA MASAYUKI

IPC: A61K49/00 ,  C07K1/22 ,  C07K14/435 ,  C12N9/64 ,  C07K15/06

Abstract: PURPOSE:To provide the subject protein reactive with anticathepsin E antibody, exhibiting protease activity, having a specific molecular weight, different from cathepsin E, secreted from pancreatic cancer cell and enabling the early diagnosis of diseases such as cancer by the determination of the concentration in body fluid. CONSTITUTION:The objective protein reactive with anticathepsin E antibody, having a molecular weight of about 60,000 as monomer and about 120,000 as dimer, different from cathepsin E, secreted from pancreatic cancer cell and exhibiting protease activity is produced by culturing pancreatic cancer cell in a medium, freeze-drying the supernatant collected from the culture product, dissolving the dried material in a 0.1M sodium acetate buffering solution (pH3.5) containing 1.0M of NaCl, passing the solution through a pepstatin-sepharose-4B column, thoroughly cleaning the column, eluting the adsorbed component with a 0.1M tris-HC1 buffering solution (pH8.6) containing 1.0M of NaCl and purifying the recovered protein solution by pepstatin affinity chromatography using pepstatin which is an acidic protease inhibitor.

公开(公告)号：JPH02129137A

公开(公告)日：1990-05-17

申请号：JP28142588

申请日：1988-11-09

Applicant: TOSOH CORP

Inventor： KUBO MASAJI ,  MATSUBA TAKAO ,  KAWABATA KOUJI

IPC: C07C43/29 ,  C08K5/06 ,  C09K21/08 ,  C09K21/14

Abstract: NEW MATERIAL:A halogenated bis(4-phenoxyphenyl) ether expressed by the formula (X is halogen; a and d are 1-5: b and c are 1-4). EXAMPLE:Octadecabromobis(4-phenoxyphenyl) ether. USE:A flame retardant of polymer material having excellent heat resistancs and migration resistance. PREPARATION:Bis(4-phenoxyphenyl) ether is brominated in the presence of a proper catalyst by a halogenating reagent such as bromine, hydrogen bromide, chloride or chlorinating agent to provide the halogenated bis(4-phenoxyphenyl) ether expressed by the formula. Halogenation degree can be controlled by amount of halogenation reagent and kind of Lewis acid catalyst.

公开(公告)号：JPH021762A

公开(公告)日：1990-01-08

申请号：JP133889

申请日：1989-01-09

Applicant: TOSOH CORP

Inventor： MATSUBA TAKAO ,  KUBO MASAJI ,  KAWAHATA MITSUTOKI

IPC: C08L23/00 ,  C08K5/54 ,  C08K5/5419 ,  C08L101/00 ,  C09K21/08

Abstract: PURPOSE:To obtain a flame-retardant high polymer composition having excellent compatibility with high polymer, heat resistance and water resistance and containing bis(pentabromophenoxy)diphenylsilane as a flame retardant capable of exhibiting high flameretardant effect by a small amount of addition thereof. CONSTITUTION:The aimed flame-retardant high polymer composition obtained by blending (A) 100 pts.wt. high polymer (e.g., polyethylene, epoxy resin or styrene-butadiene rubber) with (B) 3-100 pts.wt., preferably 10-50 pts.wt. bis(pentabromophenoxy)diphenylsilane expressed by the formula obtained by reaction of pentabromophenol with a dihalogenodiphenylsilane and as necessary flame-retardant auxiliary (e.g., antimony trioxide), other flame retardant and other additives (e.g., antioxidant, filler and crosslinking agent), having excellent flame retardance and free from breed-out of flame retardant in molding or long-term storage.

公开(公告)号：JPH01152160A

公开(公告)日：1989-06-14

申请号：JP30877287

申请日：1987-12-08

Applicant: TOSOH CORP

Inventor： MATSUBA TAKAO ,  KUBO MASAJI ,  KAGAWA TAKUMI ,  KAWABATA MITSUTOKI

IPC: C08K5/10 ,  C08L101/00 ,  C09K21/08

Abstract: PURPOSE:To obtain a polymer composition useful as a highly heat-resistant and highly weather-resistant flame retardant, by mixing a polymer with a specified bis(biphenyl) diester derivative. CONSTITUTION:This polymer composition is formed by mixing a polymer with a bis(biphenyl) diester derivative of the formula (wherein X is Br or Cl, m is 0-9, n is 0-9, m+n>1, R is a 1-4C alkylene, a phenylene, or an alkylene oxide, and H atoms contained in these groups may be substituted with Br or Cl atoms.). Examples of the compounds of the formula include bis(octabromobiphenyl) terephthalate, bis(octachlorobiphenyl) terephthalate and bis(octachlorobiphenyl)-2,3-dibromosuccinate. This derivative can be obtained by, for example, reacting a phenylphenol compound with a dicarboxylic acid dichloride.

公开(公告)号：JP2015023816A

公开(公告)日：2015-02-05

申请号：JP2013154684

申请日：2013-07-25

Applicant: 東ソー株式会社 ,  Tosoh Corp

Inventor： MUTO YU ,  MATSUBA TAKAO

IPC: C12N5/07

Abstract: 【課題】ホローファイバー培養装置は、ICS中を培地が循環している点や中空糸膜の素材が限られていることが要因となり、静置状態で成育していた細胞が必ずしも培養できる訳ではないため、培養環境の変化による細胞のダメージを低減し、ホローファイバー中で細胞培養および細胞からのタンパク質生産を行う方法を提供する。【解決手段】タンパク質を生産しうる細胞(例えば抗トリヨードサイロニンウサギモノクローナル抗体を産生するCHO−K1細胞)を、ポリスルホン系等の中空糸膜を用いたホローファイバーで培養する際に、培地中に界面活性剤を共存させて培養し、生産されたタンパク質を回収することにより、タンパク質を製造する。【選択図】図1

Abstract translation: 要解决的问题：提供一种中空纤维中的细胞培养方法和通过改变培养环境减少细胞损伤的细胞的蛋白质生产的方法，从而解决了具有中空纤维的培养装置中的问题，即在静止状态下生长的细胞 由于ICS中的培养基循环和中空纤维膜材料的限制，总是可以培养。解决方案：本发明包括通过以下方法培养能够产生蛋白质的细胞（例如产生抗三碘甲状腺原氨酸兔单克隆抗体的CHO-KI细胞） 通过使用中空纤维膜如聚砜系的中空纤维培养细胞时，在培养基中共存表面活性剂，并且通过收集蛋白质生产蛋白质。

公开(公告)号：JP2010164551A

公开(公告)日：2010-07-29

申请号：JP2009256179

申请日：2009-11-09

Applicant: Tosoh Corp ,  東ソー株式会社

Inventor： MATSUBA TAKAO

IPC: G01N33/542 ,  G01N21/64 ,  G01N21/78 ,  G01N33/15 ,  G01N33/50 ,  G01N33/577

Abstract: PROBLEM TO BE SOLVED: To provide a method of screening a material having intermolecular interaction by fluorescence polarization measurement by suppressing rise of a background, even when a material having self-fluorescence is included in an object. SOLUTION: In this screening method of the material having intermolecular interaction to a labeled substance in an object sample by measuring fluorescence polarization from the fluorescence labeled substance, an excitation wavelength (λ1) suppressing self-fluorescence of the object sample at a fluorescence measuring wavelength (λ2) is used. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：即使当具有自荧光的材料被包括在物体中时，提供通过抑制背景的上升来通过荧光偏振测量来筛选具有分子间相互作用的材料的方法。 解决方案：在通过测量来自荧光标记物质的荧光偏振的对象样品中与标记物质具有分子间相互作用的材料的筛选方法中，抑制荧光物体样品的自发荧光的激发波长（λ1） 使用测量波长（λ2）。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2006006250A

公开(公告)日：2006-01-12

申请号：JP2004190340

申请日：2004-06-28

Applicant: Mie Tlo Co Ltd ,  Tosoh Corp ,  東ソー株式会社 ,  株式会社三重ティーエルオー

Inventor： TOMITA MASAHIRO ,  MATSUBA TAKAO

IPC: C12N15/02 ,  C12N5/10 ,  C12P21/08

Abstract: PROBLEM TO BE SOLVED: To provide a method for preparing a hybridoma producing an antibody recognizing the three dimensional structure of a cellular membrane protein. SOLUTION: This method for preparing a monoclonal antibody specific to a target protein is characterized by comprising steps of mixing a group of lymphocytes obtained by immunity by using the target protein as an antigen with myeloma cells showing the protein on the surface of the cells; selectively approaching the lymphocytes showing a membrane-binding type monoclonal antibody specific to the protein in the lymphocyte group towards the myeloma cells through a specific binding of the membrane-binding type antibody with the membrane protein existing on the surface of the myeloma cells; and fusing both cells. COPYRIGHT: (C)2006,JPO&NCIPI

Abstract translation: 待解决的问题：提供一种制备识别细胞膜蛋白三维结构的产生抗体的杂交瘤的方法。 解决方案：用于制备针对靶蛋白特异性的单克隆抗体的方法的特征在于包括以下步骤：将通过使用靶蛋白作为抗原的免疫获得的一组淋巴细胞与在所述蛋白表面上显示蛋白质的骨髓瘤细胞混合 细胞; 选择性地接近淋巴细胞，其通过膜结合型抗体与存在于骨髓瘤细胞表面上的膜蛋白的特异性结合，显示对淋巴细胞组中蛋白质特异性的膜结合型单克隆抗体朝向骨髓瘤细胞; 并融合两个细胞。 版权所有（C）2006，JPO＆NCIPI