公开(公告)号：JPS5687541A

公开(公告)日：1981-07-16

申请号：JP16360379

申请日：1979-12-18

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KITA JIYUNICHIROU ,  JIBIKI HIROSHI ,  KAKEYA NOBORU ,  FUJIMURA SUSUMU ,  OKIMOTO KIYOMI

IPC: C07B61/00

Abstract: PURPOSE:To obtain the titled compound useful as an intermediate of pharmaceuticals and agricultural chemicals in high yield, by the reaction of a cyanoformate and an active methylene compound in the presence of the reaction product of stannous chloride and an N-halo compound, followed by treatment with water, etc. CONSTITUTION:A compound of formula I (R is 1-4C alkyl) and an active methylene compound of formula II [R is CN, COR (R is 1-4C alkyl or 6-14C aryl), etc.; R is H, CN, COR , etc.] are made to react in the presence of the reaction product of stannous chloride and an N-halo compound selected from the group of N-halobenzenesulfonamide, N-halo-epsilon-caprolactam and N-halosuccinimide. The product obtained is treated with water or an acidic aqueous solution (e.g. hydrochloric acid, sulfuric acid, etc.) at 0-35 deg.C to give a compound of formula III (e.g. 1-amino-2-cyano-1,2-dimethoxycarbonylethylene).

公开(公告)号：JPH06217762A

公开(公告)日：1994-08-09

申请号：JP1151093

申请日：1993-01-27

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KUBO FUMIO ,  KUDO ISAO ,  UMEDA KAZUHIRO ,  IWAMOTO YOSHITADA ,  OKA YURIO

IPC: C12N1/00 ,  C12N1/20 ,  C12R1/19

Abstract: PURPOSE:To provide a high-concentration and high-efficiency culture method for coli bacillus, capable of efficiently producing the objective substance in production of an enzyme, a bioactive substance, etc., by coli bacillus. CONSTITUTION:In relation to aerobic culture of coli bacillus, this high- concentration and high-efficiency culture method is carried out by adding an amino acid composition to the culture medium so that amino acids required for growth of the coli bacillus may not run short and simultaneously adding glucose to the medium so as to control pH of the medium to be a constant value in a state where pH of the medium increases.

公开(公告)号：JPH06209771A

公开(公告)日：1994-08-02

申请号：JP904093

申请日：1993-01-22

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KUBO FUMIO ,  TANAKA MASAYUKI ,  IWAMOTO YOSHITADA

IPC: C12N9/02 ,  C12N15/09 ,  C12N15/10 ,  C12P21/00 ,  C12R1/19

Abstract: PURPOSE:To separate, from a solution containing a DNA obtained by cell homogenization of a microorganism and a biologically active protein, the DNA and the biologically active protein by using a resin bound with anion exchanging groups. CONSTITUTION:An E. coli DNA and a biologically active protein such as human SOD are separated by using a resin bound with anion exchanging groups such as HPA-25 from a buffer solution of 10mM and pH7.0 containing the DNA obtained by cell homogenization of recombinant E. coli. and the biologically active protein.

公开(公告)号：JPH01304882A

公开(公告)日：1989-12-08

申请号：JP13545788

申请日：1988-06-03

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  WATANABE MASAYUKI

IPC: C12N9/96

Abstract: PURPOSE:To stably store human superoxide dismutase of Cu, Zn-type (human SOD) in frozen state, etc., by mixing human SOD with at least one of disaccharides, monosaccharides such as ketose or sugaralcohol. CONSTITUTION:At least one sugar of disaccharides, monosaccharides such as ketose or sugaralcohol and human SOD are mixed and stored, thus human SOD is stably stored in frozen state or freeze-dried state. Using amount of sugar is preferably 0.05-10 times of the amount of human SOD, more preferably 0.1-6 times. Concrete example of human SOD is SOD extracted from cell, texture and organ, etc., of human and purified, one produced by microorganism with intragenic recombination and having same sequence of amino acid as human SOD. Mixing of human SOD and sugar is carried out at 0-40 deg.C, more preferably 0-15 deg.C.

公开(公告)号：JPS5890563A

公开(公告)日：1983-05-30

申请号：JP18689481

申请日：1981-11-24

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KITA JIYUNICHIROU ,  KAKEYA NOBORU ,  YOKOO YASUHIKO

IPC: C07D239/557 ,  C07D239/54

Abstract: PURPOSE:The reaction of an alkali metal salt of 5-carboxyorotic acid with a halogenating reagent is followed by treatment of the product with an acid to give the titled compound, especially 5-iodoorotic acid, used as a medicine or its starting material, in high yield. CONSTITUTION:A halogenating reagent is added as much, or in the form of an aqueous solution or solution in a inert organic solvent, to an aqueous solution of an alkali metal salt of 5-carboxyorotic acid of formulaI(M is Li, Na, K) and their reaction is effected by heating at 0-100 deg.C and the resultant reaction product is acidified with a mineral acid at 0-30 deg.C to give the objective compound. As a halogenating reagent, is used Br2, I2 or N-chlorosuccinimide. The compound of formulaIis obtained by making 3-amino-1,4-bis(alkoxycarbonyl) maleimide of formula III (R , R are 1-4C alkyl) react with an alkali hydroxide in water at room temperature or under heating and acidifying the reaction mixture with a mineral acid.

公开(公告)号：JPS5888361A

公开(公告)日：1983-05-26

申请号：JP18689381

申请日：1981-11-24

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KITA JIYUNICHIROU ,  YOKOO YASUHIKO

IPC: C07D207/456

Abstract: NEW MATERIAL:3-Amino-1,4-bis(alkoxycarbonyl)maleimide compound of formulaI (R and R are 1-4C alkyl). EXAMPLE:3-Amino-1,4-bis(ethoxycarbonyl)maleimide. USE:Phramaceuticals, agricultural chemicals, and their intermediates. PROCESS:The compound of formulaIcan be prepared by reacting the alkoxyiminoacetic acid ester of formula II (R and R are 1-4C alkyl) with the N- (alkoxycarbonyl)acetylcarbamic acid ester of formula III in the absence of solvent or in an organic solvent at 20-150 deg.C for 1-50hrs.

公开(公告)号：JPS5888360A

公开(公告)日：1983-05-26

申请号：JP18689281

申请日：1981-11-24

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KITA JIYUNICHIROU ,  FUJIMURA SUSUMU ,  MATSUO FUMIO

IPC: C07D207/456 ,  A01N37/32

Abstract: NEW MATERIAL:The compound of formulaI(R is 1-4C alkyl or halogen; R is phenyl substituted with 1-4C alkyl or alkoxy; R is 2-5C alkoxycarbonyl). EXAMPLE:1-(p-Chlorophenyl)-3-ethoxycarbonyl-4-(p-toluidino)maleimide. USE:Agricultural chemicals, pharmaceuticals and their intermediates. PROCESS:The compound of formulaIcan be prepared by reacting the carbamoylaminomaleimide compound of formula II with a tertiary amine in an inert organic solvent under essentially anhydrous conditions at 20-150 deg.C for 1-50hrs.

公开(公告)号：JPS5862175A

公开(公告)日：1983-04-13

申请号：JP15949981

申请日：1981-10-08

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KAKEYA NOBORU ,  JIBIKI HIROSHI ,  MATSUO FUMIO

IPC: C07D487/04 ,  A01N43/90

Abstract: NEW MATERIAL:An imidazopyrimidine shown by the formulaI[R is benzoyl, acetyl, or ethoxycarbonyl; R is 1-4C alkyl or a group shown by the formula II (R is 1-4C alkyl or 1-4C alkoxy; n is 0, 1, or 2)]. EXAMPLE:8-Benzoyl-2,6-diethyl-1,2,3,5,6,7-hexahydro-1,3,5,7-tetraoxoim idazo[1,5- C]pyrimidine. USE:An agricultural chemical, drug, or an intermediate for them. PROCESS:An alpha,beta-unsaturated-alpha-amino ethyl ester shown by the formula III is reacted with an isocyanate shown by the formula IV in the presence of a base to give a compound shown by the formulaI.

公开(公告)号：JPS5862164A

公开(公告)日：1983-04-13

申请号：JP15949781

申请日：1981-10-08

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KITA JIYUNICHIROU ,  OKIMOTO KIYOMI

IPC: C07D233/96

Abstract: PURPOSE:To obtain the titled substance useful as a drug, agricultural chemical, etc. in high yield, by reacting a reaction product of an alkoxyiminoacetic ester with an amine with an isocyanate in the presence of a tertiary amine and/or quaternary ammonium fluoride. CONSTITUTION:A compound shown by the formulaI(R and R are 1-4C alkyl) is reacted with a compound shown by the formula II (R is 1-4C alkyl, etc.) in the presence or absence of an inert organic solvent (alcohol, aromatic hydrocarbon, etc.) at 0-150 deg.C to give a compound shown by the formula V, preferably after the removal of an alcohol as a by-product from the reaction product mixture, the compound is reacted with a compound shown by the formula III (R is 1-4C alkyl, etc.) in the presence of a tertiary amine (e.g., trimethylamine) and/or quaternary ammonium fluoride at 20-100 deg.C for 1-30hr, to give a compound shown by the formula IV.

公开(公告)号：JPS5862150A

公开(公告)日：1983-04-13

申请号：JP15950081

申请日：1981-10-08

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  MATSUO FUMIO

IPC: C07C257/22 ,  C07C67/00 ,  C07C239/00 ,  C07C257/10 ,  C07C301/00 ,  C07C303/36 ,  C07C311/49

Abstract: PURPOSE:To prepare the titled compound useful as pharmaceuticals, etc., in high yield, without producing stenchy compound in contrast with conventional process, by reacting an alkoxyiminoacetic acid ester with a hydrazine compound. CONSTITUTION:The objective compound of formula II is prepared by reacting an alkoxyiminoacetic acid ester of formulaI(R and R are 1-4C alkyl) with a hydrazine compound of formula R NHNH2 (R is 1-5C aliphatic acyl, benzoyl, etc.) in the presence of a solvent such as methanol at 0-100 deg.C usually for 1-3 days. EFFECT:A complicated deodorizing reactor is not necessary.