公开(公告)号：MX2007004068A

公开(公告)日：2007-06-04

申请号：MX2007004068

申请日：2005-10-04

Applicant: UNIV CALIFORNIA

Inventor： BACHMAN MARK ,  LI GUANN-PYNG ,  ALLBRITTON NANCY ,  SIMS CHRISTOPHER

IPC: C12M1/00 ,  C12Q1/00

Abstract: Una placa fabricada para permitir que muestras de celulas, microorganismos, proteinas, ADN, biomoleculas y otros medios biologicos se coloquen en ubicaciones o sitios especificos sobre la placa con el proposito de realizar analisis direccionables en las muestras; de preferencia, algunos o todos los sitios se construyen a partir de un material removible o como tarimas para que un subconjunto de las muestras de interes se pueda aislar facilmente de la placa para procesamiento o analisis posterior; la placa puede contener estructuras o tratamientos quimicos que mejoran o promueven la fijacion y/o funcion de las muestras, y que promueven o ayudan en sus analisis.

公开(公告)号：AU2003234323A1

公开(公告)日：2003-11-17

申请号：AU2003234323

申请日：2003-05-02

Applicant: UNIV CALIFORNIA

Inventor： BACHMAN MARK ,  SIMS CHRISTOPHER ,  HAN FUTIAN ,  ALBRITTON NANCY ,  LI GUANN-PYNG

IPC: B01L3/00 ,  G01N27/447 ,  G01N33/50 ,  G01N33/559 ,  G01N33/561

公开(公告)号：AU2002342599A1

公开(公告)日：2002-11-25

申请号：AU2002342599

申请日：2002-05-09

Applicant: UNIV CALIFORNIA

Inventor： ALLBRITTON NANCY ,  SIMS CHRISTOPHER

IPC: G01N27/62 ,  B01D53/62 ,  B01D57/02 ,  B03C5/00 ,  C08F2/58 ,  C12M1/26 ,  C12M1/34 ,  C12M1/42 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/48 ,  G01N27/447 ,  G01N27/48 ,  G01N33/483 ,  G01N33/50 ,  C02F1/26 ,  C25B15/00 ,  B01D59/50 ,  B01D61/58 ,  G01L1/20 ,  G01F1/64 ,  B01D59/42 ,  C25B7/00 ,  B01D61/42 ,  G01L9/18 ,  G01N27/26

Abstract: The activity of oncogenic intracellular chemical reactions of molecules is measured by the use of fluorescently labeled substrate molecules that undergo a change in electrophoretic mobility upon a chemical reaction such as that catalyzed by an enzyme or kinase. Specificity is achieved by using labeled substrate molecules that can be acted upon only by specific oncogenic enzymes. Thus the activity of an oncogenic enzyme or class of oncogenic enzymes can be determined. Measurements are made with the intracellular presence of such substrate molecules, at some time of interest. To ensure accuracy, measurements must be made in a timely manner so as to minimize chemical reactions occurring subsequent to the time of interest. Fast controllable laser lysis is used to obtain the contents of said cell or cells into which reporter substrate molecules have been introduced. The cell contents are then subjected to capillary electrophoresis and oncogenic enzymatic activity is determined by comparing amounts of unaltered substrate molecules to the amounts of altered substrate molecules which are separated by the electrophoresis and identified by the presence of a fluorescent label.

公开(公告)号：AU1783902A

公开(公告)日：2002-06-03

申请号：AU1783902

申请日：2001-11-21

Applicant: UNIV CALIFORNIA

Inventor： ALLBRITTON NANCY L ,  SIMS CHRISTOPHER

IPC: B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/42 ,  C12Q1/02 ,  G01N1/02 ,  G01N15/14 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/68