Abstract:
The present invention provides a method for inducing an inversion of normal blood coagulation factor VIII (F8) gene, a method for correcting an inversion of blood coagulation factor VIII gene in which the inversion has occurred, and a Hemophilia A patient-derived induced pluripotent stem cell in which the inversion is corrected, constructed using the same. The method of the present invention effectively reproduces the inversion of intron 1 and intron 22 of the F8 gene, which is responsible for the majority of severe hemophilia A, and thereby may be effectively used for studying the development mechanism of hemophilia A and as a research tool for screening therapeutic agents. The inversion-corrected induced pluripotent stem cell constructed according the method of the present invention enables an efficient and fundamental treatment for hemophilia A by restoring a genotype in which mutation has occurred to a wild type-like state, without limitation via normal gene or protein delivery.
Abstract:
The present invention relates to a apparatus and method for measuring a waveform of a light wave. A light wave measurement apparatus according to an embodiment of the present invention includes a pulse separation unit to separate an input light wave into a fundamental pulse and a signal pulse, a time delay adjustment unit to adjust a time delay between the fundamental pulse and the signal pulse, a focusing unit to focus the fundamental pulse and the signal pulse whose time delay is adjusted on an ionization material, and an ionization yield measurement unit to measure an ionization yield from electrons and/or ions generated by the focused fundamental pulse and signal pulse. The waveform of the input light wave is obtained by obtaining an ionization yield modulation changed by the signal pulse as a function of the time delay.
Abstract:
The present invention relates to a method for increasing the production efficiency of genome-modified plants regenerated from plant protoplasts by using a Cas protein-guide RNA ribonucleoprotein (RNP). The method for increasing the production efficiency of genome-modified plants of the present invention can efficiently produce plants with mutated target genes, and can also minimize the insertion of foreign DNA into plants. Therefore, the present invention can be very usefully used in various fields such as agriculture, food and biotechnology fields.
Abstract:
The present invention relates to a method for increasing the production efficiency of genome-modified plants regenerated from plant protoplasts by using a Cas protein-guide RNA ribonucleoprotein (RNP). The method for increasing the production efficiency of genome-modified plants of the present invention can efficiently produce plants with mutated target genes, and can also minimize the insertion of foreign DNA into plants. Therefore, the present invention can be very usefully used in various fields such as agriculture, food and biotechnology fields.
Abstract:
The present invention relates to a cell sheet manufacturing device and a manufacturing method therefor. More specifically, the present invention relates to a cell sheet manufacturing device comprising a support layer made of silicon rubber, a patterned electrode formed adjacent to the support layer and a graphene layer formed adjacent to the electrode, and a manufacturing method therefor.
Abstract:
The present invention relates to a pharmaceutical composition for preventing or treating attention deficit/hyperactivity disorder (ADHD), comprising SNAP5114 as an active ingredient. A Git1 gene-deficient hetero (+/-) mouse, confirmed to have increased amount of GABA in the brain striatum that controls hyperactivity, can be used as an animal model of ADHD, and by confirming that hyperactivity is ameliorated by administering SNAP5114 to the Git1 gene-deficient hetero (+/-) mouse, SNAP5114 is provided as a therapeutic agent for ADHD.
Abstract:
Proposed is a fuel cell stack including a plurality of fuel cell units having a truncated cone shape and connected in series with each other. The series connection of the fuel cell units is made such that a relatively small outer diameter portion of one of the fuel cell units is inserted into a relatively large outer diameter portion of another fuel cell unit adjacent to the former fuel cell unit.
Abstract:
The present invention relates to at least one delivery vehicle comprising: either a plurality of cleavaging agents that specifically recognize a plurality of nucleic acid sequences including unique sequences, which do not exist in normal cells, among variant region sequences specific for cells having genomic sequence variation in the cells having genomic sequence variation, or polynucleotides encoding the cleavaging agents; and a nuclease or a polynucleotide encoding the nuclease. The present invention also relates to a composition containing the delivery vehicle, and a method of inducing apoptosis of cells having genomic sequence variation by using the delivery vehicle or the composition.
Abstract:
Provided are 3D morphological or anatomical landmark detection method and device using deep reinforcement learning, in which the method includes performing volume rendering on 3D image data to generate 3D volume rendered model data, and applying a single-stage deep reinforcement learning (DRL), including obtaining a 2D coordinate value corresponding to a landmark point on a 2D view image, which is constructed by the 3D volume rendered model data, by using a deep reinforcement learning agent, and obtaining a remaining 1D coordinate value corresponding to the landmark point based on an 1D profile of the 3D image data which is obtained based on the 2D coordinate value. The method may further include applying a multi-stage DRL, including detecting the landmark point by repeating the operations of obtaining the 2D coordinate value corresponding to the landmark point on the 2D view image, which is constructed by the 3D volume rendered model data, by using the deep reinforcement learning agent, and, from a 2D view image which is determined based on the obtained 2D coordinate value, obtaining a 2D coordinate value corresponding to the landmark point by using a deep reinforcement learning agent.