公开(公告)号：WO2015035087A1

公开(公告)日：2015-03-12

申请号：PCT/US2014/054146

申请日：2014-09-04

Applicant: FLUIDIGM CORPORATION

Inventor： EGIDIO, Camila ,  RAMAKRISHNAN, Ramesh ,  RUFF, David

IPC: C12Q1/68

CPC classification number: C12Q1/6804 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q2563/179 ,  C12Q2565/543

Abstract: Methods and reagents for detection and analysis of nucleic acids and proteins using proximity extension assays.

Abstract translation: 使用接近延伸测定法检测和分析核酸和蛋白质的方法和试剂。

公开(公告)号：WO2011142836A2

公开(公告)日：2011-11-17

申请号：PCT/US2011/000887

申请日：2011-05-16

Applicant: FLUIDIGM CORPORATION ,  JONES, Robert, C. ,  LIVAK, Kenneth, J. ,  MAY, Andrew ,  MIR, Alain ,  PIEPRZYK, Martin ,  QIN, Jian ,  RAMAKRISHNAN, Ramesh ,  SPURGEON, Sandra ,  WANG, Jun ,  ZIMMERMANN, Bernhard, G.

Inventor： JONES, Robert, C. ,  LIVAK, Kenneth, J. ,  MAY, Andrew ,  MIR, Alain ,  PIEPRZYK, Martin ,  QIN, Jian ,  RAMAKRISHNAN, Ramesh ,  SPURGEON, Sandra ,  WANG, Jun ,  ZIMMERMANN, Bernhard, G.

IPC: C12Q1/68 ,  G01N33/52 ,  G06F19/18

CPC classification number: C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  G01N33/542 ,  G01N33/582 ,  G01N33/6893 ,  G01N2800/368 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2535/131 ,  C12Q2537/143 ,  C12Q2537/16 ,  C12Q2563/173

Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.

Abstract translation: 本发明提供用于检测基因型，突变和/或非整倍体的基于扩增的方法。 这些方法具有广泛的适用性，但是特别适合于检测和定量存在于母体体液样品中的游离胎儿DNA中的靶核酸。

公开(公告)号：WO2011066588A1

公开(公告)日：2011-06-03

申请号：PCT/US2010/058459

申请日：2010-11-30

Applicant: FLUIDIGM CORPORATION ,  WANG, Jing ,  WOUDENBERG, Timothy M.

Inventor： WANG, Jing ,  WOUDENBERG, Timothy M.

IPC: A61L2/00

CPC classification number: B08B9/027 ,  B01L3/5027 ,  B01L99/00 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  F16K99/0001 ,  F16K99/0026 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084

Abstract: A method for rendering a microfluidic device suitable for reuse for nucleic acid analysis is provided. The method may include flowing a nucleic acid inactivating solution into a microfluidic channel of the device by pumping; and then flowing a wash solution into the channel by pumping, thereby displacing the nucleic acid inactivating solution from the channel, whereby any residual nucleic acid from a prior use of the device is inactivated.

Abstract translation: 提供了一种用于制备适于重新用于核酸分析的微流体装置的方法。 该方法可以包括通过泵送将核酸灭活溶液流动到装置的微流体通道中; 然后通过泵送将洗涤溶液流入通道，​​从而使来自通道的核酸灭活溶液移位，由此来自先前使用该装置的任何残留核酸被灭活。

公开(公告)号：WO2010077618A1

公开(公告)日：2010-07-08

申请号：PCT/US2009/067037

申请日：2009-12-07

Applicant: FLUIDIGM CORPORATION ,  FOWLER, Brian

Inventor： FOWLER, Brian

IPC: B01L99/00

CPC classification number: B01L3/502784 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00398 ,  B01J2219/00418 ,  B01L7/525 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/089 ,  B01L2300/1805 ,  B01L2400/0427

Abstract: A microfluidic device includes a pressure source and a control line in fluid communication with the pressure source. The microfluidic device also includes a plurality of valves operated via the control line and an independent valve positioned adjacent the control line and between the pressure source and the plurality of valves.

Abstract translation: 微流体装置包括压力源和与压力源流体连通的控制管线。 微流体装置还包括经由控制管线操作的多个阀和邻近控制管线定位并且在压力源和多个阀之间的独立阀。

公开(公告)号：WO2009105609A1

公开(公告)日：2009-08-27

申请号：PCT/US2009/034635

申请日：2009-02-20

Applicant: FLUIDIGM CORPORATION ,  AMIN, Yusuf, D

Inventor： AMIN, Yusuf, D

IPC: B81B1/00 ,  B81B7/00 ,  H01L21/44 ,  G01N27/00 ,  B01L3/00

CPC classification number: B01J19/0046 ,  B01L3/502707 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/043 ,  B01L2300/123 ,  B01L2300/1805

Abstract: A carrier for holding a microfluidic device includes a substrate with a plurality of wells, each well defining a volume of between 0.1 µl and 100 µl; a plurality of channels within the substrate wherein each well is in fluid communication with at least one of the plurality of channels; and a receiving portion for receiving a microfluidic device and placing the microfluidic device in fluid communication with the plurality of wells. The carrier has a polymeric composition and/or an array of structural features that enhance its performance and compatibility with existing instrumentation.

Abstract translation: 用于保持微流体装置的载体包括具有多个孔的基底，每个孔限定0.1μl和100μl之间的体积; 衬底内的多个通道，其中每个阱与所述多个通道中的至少一个流体连通; 以及用于接收微流体装置并将微流体装置与多个孔流体连通的接收部分。 载体具有增强其性能和与现有仪器的兼容性的聚合物组合物和/或结构特征阵列。

公开(公告)号：WO2007044091A2

公开(公告)日：2007-04-19

申请号：PCT/US2006/021416

申请日：2006-06-02

Applicant: FLUIDIGM CORPORATION ,  HEID, Christian, A. ,  DARIDON, Antoine

Inventor： HEID, Christian, A. ,  DARIDON, Antoine

IPC: C12Q1/68 ,  C12M3/00

CPC classification number: B01L3/5027 ,  B01L7/52 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/301

Abstract: The invention relates to methods, reagents and devices for detection and characterization of nucleic acids, cells, and other biological samples. Assay method are provided in which a sample is partitioned into sub-samples, and analysis of the contents of the sub-samples carried out. The invention also provides microfluidic devices for conducting the assay. The invention also provides an analysis method using a universal primers and probes for amplification and detection.

Abstract translation: 本发明涉及核酸，细胞和其他生物样品的检测和表征的方法，试剂和装置。 提供了将样品分割成子样品并进行子样品的内容的分析的测定方法。 本发明还提供了用于进行测定的微流体装置。 本发明还提供了使用通用引物和探针进行扩增和检测的分析方法。

公开(公告)号：WO2007033385A2

公开(公告)日：2007-03-22

申请号：PCT/US2006/036365

申请日：2006-09-13

Applicant: FLUIDIGM CORPORATION ,  NASSEF, Hany, Ramez ,  CHOU, Hou-pu ,  LUCERO, Michael ,  MAY, Andrew ,  YOKOBATA, Kathy

Inventor： NASSEF, Hany, Ramez ,  CHOU, Hou-pu ,  LUCERO, Michael ,  MAY, Andrew ,  YOKOBATA, Kathy

CPC classification number: G01N33/54366 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00389 ,  B01J2219/00398 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0655 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N33/5302

Abstract: A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.

Abstract translation: 适用于执行许多同时结合测定的微流体装置，包括但不限于免疫学实验，如ELISA测定，在一级和二级抗体之间具有最小的串扰。

公开(公告)号：WO2006102264A1

公开(公告)日：2006-09-28

申请号：PCT/US2006/010103

申请日：2006-03-20

Applicant: FLUIDIGM CORPORATION ,  UNGER, Marc, A. ,  MCBRIDE, Lincoln ,  FACER, Geoffrey

Inventor： UNGER, Marc, A. ,  MCBRIDE, Lincoln ,  FACER, Geoffrey

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC classification number: C12Q1/6818 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0655 ,  C12Q2565/1015 ,  C12Q2533/101 ,  C12Q2525/301

Abstract: Devices and methods for performing the relative concentration of a target in a sample, the sample containing both target and non-target components, the method performed by partitioning the sample into a large number of reaction volumes such that the target is concentrated relative to the non-target, and performing a detection assay upon each reaction volume to detect the target.

Abstract translation: 用于执行样品中靶标相对浓度的装置和方法，含有靶和非目标成分的样品，通过将样品分成大量反应体积进行的方法，使得靶相对于非目标成分集中 并且在每个反应体积上进行检测测定以检测目标。

公开(公告)号：WO2020257258A1

公开(公告)日：2020-12-24

申请号：PCT/US2020/038097

申请日：2020-06-17

Applicant: FLUIDIGM CORPORATION ,  FLUIDIGM CANADA INC.

Inventor： CORKUM, Paul ,  LOBODA, Alexander

IPC: G01N27/62 ,  G01N15/10 ,  G01N21/71 ,  H01J49/04 ,  H01J49/26 ,  H01J49/40

Abstract: Embodiments of the present invention relate to replacement of the previous ICP-based ionisation system with a new laser ionisation system, providing improved mass spectrometer-based apparatus and methods for using them to analyse samples, in particular the use of mass spectrometry mass cytometry, imaging mass spectrometry and imaging mass cytometry, for the analysis of biological samples. Accordingly, embodiments of the present invention provide an apparatus, for example a mass cytometer, comprising: 1 ) a sampler; 2) a laser ionisation system to receive material removed from the sample by the sampler, wherein the laser ionisation system comprises an ionisation system conduit and a pulsed laser adapted to ionise sample material passing through or exiting the ionisation system conduit; and 3) a mass spectrometer to receive elemental ions from said ionisation system and to analyse said elemental ions.

公开(公告)号：WO2019246033A1

公开(公告)日：2019-12-26

申请号：PCT/US2019/037644

申请日：2019-06-18

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION ,  CORKUM, Paul

Inventor： CORKUM, Paul ,  LOBODA, Alexander

IPC: H01J49/00 ,  H01J49/40 ,  G01N33/574 ,  G01N33/60 ,  G01N33/68 ,  G02B21/33 ,  G02B21/26 ,  G02B21/02

Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMCTM) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.