公开(公告)号：WO2005042756A2

公开(公告)日：2005-05-12

申请号：PCT/US2004/034558

申请日：2004-10-21

Applicant: CARGILL, INC. ,  MCFARLAN, Sara C. ,  HICKS, Paula M. ,  ZIDWICK, Mary Jo ,  SANCHEZ-RIERA, Fernando A. ,  CAMERON, Douglas C. ,  DESOUZA, Mervyn L. ,  ROSAZZA, Jack ,  GORT, Steven J.

Inventor： MCFARLAN, Sara C. ,  HICKS, Paula M. ,  ZIDWICK, Mary Jo ,  SANCHEZ-RIERA, Fernando A. ,  CAMERON, Douglas C. ,  DESOUZA, Mervyn L. ,  ROSAZZA, Jack ,  GORT, Steven J.

IPC: C12P13/04

CPC classification number: C12N9/88 ,  C12P17/10 ,  Y02P20/582

Abstract: Methods and compositions that can be used to make monatin or salt thereof from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.

Abstract translation: 可用于从葡萄糖，色氨酸，吲哚-3-乳酸，吲哚-3-丙酮酸和2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸制备莫纳甜或其盐的方法和组合物 ，提供。 还公开了制备吲哚-3-丙酮酸和2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸中间体的方法。 提供的组合物包括核酸分子，多肽，化学结构和细胞。 方法包括体外和体内过程，体外方法包括化学反应。

公开(公告)号：WO2005016022A1

公开(公告)日：2005-02-24

申请号：PCT/US2004/026330

申请日：2004-08-16

Applicant: CARGILL, INCORPORATED ,  HICKS, Paula, M.

Inventor： HICKS, Paula, M.

IPC: A23G3/30

CPC classification number: A23G4/06 ,  A23G4/10 ,  A23G4/14

Abstract: Chewing gum compositions are described that include one or more stereoisomers of monatin, a naturally occurring high-intensity sweetener.

Abstract translation: 描述了口香糖组合物，其包括monatin的一种或多种立体异构体，天然存在的高强度甜味剂。

公开(公告)号：WO2009088442A3

公开(公告)日：2010-03-18

申请号：PCT/US2008013968

申请日：2008-12-22

Applicant: CARGILL INC ,  DE SOUZA MERVYN L ,  NIU WEI ,  LAPLAZA JOSE M ,  SOLHEID CHRISTOPHER ,  KOLLMANN SHERRY R ,  LUNDORFF JOSHUA M ,  HICKS PAULA M ,  MCFARLAN SARA C ,  SANCHEZ-RIERA FERNANDO A ,  WEINER DAVID P ,  BURKE ELLEN ,  LUGINBUHL PETER ,  BUENO ANALIA ,  CUENCA JOSLIN

Inventor： DE SOUZA MERVYN L ,  NIU WEI ,  LAPLAZA JOSE M ,  SOLHEID CHRISTOPHER ,  KOLLMANN SHERRY R ,  LUNDORFF JOSHUA M ,  HICKS PAULA M ,  MCFARLAN SARA C ,  SANCHEZ-RIERA FERNANDO A ,  WEINER DAVID P ,  BURKE ELLEN ,  LUGINBUHL PETER ,  BUENO ANALIA ,  CUENCA JOSLIN

IPC: C12Q1/68 ,  C12N9/90

CPC classification number: C12P13/04 ,  C12P13/227

Abstract: The invention provides for isomerase (e.g., racemase) and epimerase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such isomerase (e.g., racemase) and epimerase nucleic acids and polypeptides.

Abstract translation: 本发明提供异构酶（例如消旋酶）和差向异构酶多肽以及编码这种多肽的核酸。 还提供了使用这种异构酶（例如消旋酶）和差向异构酶核酸和多肽的方法。

公开(公告)号：WO2007140195A2

公开(公告)日：2007-12-06

申请号：PCT/US2007069513

申请日：2007-05-23

Applicant: CARGILL INC ,  HILBERT BRENT H ,  SOLHEID CHRISTOPHER ,  MCFARLAN SARA C ,  KHARE ANIL B ,  DESOUZA MERVYN ,  SANCHEZ-RIERA FERNANDO A ,  FLOY KENT ,  HICKS PAULA M

Inventor： HILBERT BRENT H ,  SOLHEID CHRISTOPHER ,  MCFARLAN SARA C ,  KHARE ANIL B ,  DESOUZA MERVYN ,  SANCHEZ-RIERA FERNANDO A ,  FLOY KENT ,  HICKS PAULA M

IPC: B01J8/00 ,  C07D209/04

CPC classification number: C07D209/20 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  C12P17/10

Abstract: Methods and systems for increasing the production of equilibrium reactions are described. In some embodiments, a method comprises removing enzymes from a reaction mixture once equilibrium is achieved, selectively re-adding enzymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-products back into the reaction mixture. In some embodiments, a method comprises purifying the desired product from a reaction mixture and selectively recycling one or more components of the reaction mixture, such as original reactants, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also provided. In some embodiments the methods and systems are implemented for increasing the production of monatin and monatin derivatives, produced in multi-step equilibrium pathways.

Abstract translation: 描述了用于增加平衡反应生成的方法和系统。 在一些实施方案中，一种方法包括一旦获得平衡即从反应混合物中除去酶，选择性地重新加入用于将产物形成反应推向产物的酶和反应物，以及任选地回收稳定的中间体，副产物和/或副产物 回到反应混合物中。 在一些实施方案中，方法包括从反应混合物中纯化所需产物，并根据需要选择性地将反应混合物中的一种或多种组分（例如原始反应物，中间体，副产物或副产物）循环回到反应混合物中，以改善 滴度。 还提供了实现方法的系统。 在一些实施方案中，实施方法和系统用于增加在多步平衡途径中产生的莫纳甜和莫纳甜衍生物的产生。

公开(公告)号：WO2005014839A3

公开(公告)日：2005-06-02

申请号：PCT/US2004024886

申请日：2004-08-02

Applicant: CARGILL INC ,  HICKS PAULA M

Inventor： HICKS PAULA M

IPC: A23L27/20 ,  A21D13/08 ,  A23C9/154 ,  A23F3/00 ,  A23F3/30 ,  A23F5/00 ,  A23G1/00 ,  A23G3/34 ,  A23L2/02 ,  A23L2/60 ,  A23L9/10 ,  A23L27/00 ,  A23L27/30 ,  C12P17/10 ,  A23L1/236 ,  A23G3/00 ,  A23L1/187

CPC classification number: A23C9/1544 ,  A21D13/80 ,  A23F3/30 ,  A23G1/56 ,  A23L2/60 ,  A23L27/31 ,  A23L27/32 ,  A23G2200/06

Abstract: The present invention relates to novel sweetener compositions comprising monatin and methods for making such compositions. The present invention also relates to sweetener compositions comprising specific monatin stereoisomers, specific blends of monatin stereoisomers, and /or monatin produced via a biosynthetic pathway in vivo (e.g., inside cells) or in vitro.

Abstract translation: 本发明涉及包含莫纳甜的新型甜味剂组合物和制备这种组合物的方法。 本发明还涉及包含特定莫纳甜立体异构体，莫纳甜立体异构体的特异性共混物和/或通过体内生物合成途径（例如细胞内）或体外产生的莫纳甜的甜味剂组合物。