公开(公告)号：KR1020070111409A

公开(公告)日：2007-11-21

申请号：KR1020070095740

申请日：2007-09-20

Applicant: 강원대학교산학협력단

Inventor： 조동하 ,  김명조 ,  진쳉유 ,  류승원 ,  유창연 ,  이범구 ,  이명구 ,  조재열

IPC: A23L1/30 ,  A23L1/29

CPC classification number: A23L33/105 ,  A23V2200/302 ,  A23V2250/21

Abstract: A kaempferol compound isolated from a kenaf extract(Hibiscus cannabinus) is provided to be used as active components of functional food with antibacterial and antioxidant activity. A functional food contains 0.1 to 20% by weight of a kaempferol compound represented by the formula(1) as an effective component from a kenaf extract and an excipient. The kaempferol compound is prepared by the steps of: obtaining crude extract by extracting kenaf leaf with methanol; obtaining a water layer and organic layer by extracting the crude extract with a solvent selected from water, aliphatic alcohol, aqueous alcohol solutions, hexane, chloroform, ethyl acetate, benzene and butanol; obtaining active fractions by absorbing the water layer and organic layer into a first silica chromatography column and eluting a fraction while changing the ratio of toluene and acetone or acetone and methanol; obtaining active fractions by absorbing the active fractions into a second silica chromatography column and eluting a fraction while changing the ratio of water and methanol(10:0 to 5:5); subjecting the fractions to gel filter chromatography(Sephadex LH-20) with 40% methanol; and finally recrystallizing the purified fractions and purifying a single active compound by high speed liquid chromatography.

Abstract translation: 提供从洋麻提取物（木槿大麻）中分离出的山奈酚化合物作为具有抗菌和抗氧化活性的功能性食品的活性成分。 功能性食品含有0.1〜20重量％的由式（1）表示的山奈酚化合物作为来自洋麻提取物和赋形剂的有效成分。 山奈酚化合物通过以下步骤制备：通过用甲醇提取洋麻叶来获得粗提物; 通过使用选自水，脂族醇，醇溶液，己烷，氯仿，乙酸乙酯，苯和丁醇的溶剂提取粗提取物得到水层和有机层; 通过将水层和有机层吸收到第一硅胶色谱柱中并在改变甲苯和丙酮或丙酮和甲醇的比例的同时洗脱级分获得活性级分; 通过将活性级分吸收到第二硅胶色谱柱中并在改变水和甲醇的比例（10:0至5:5）的同时洗脱级分来获得活性级分; 用馏分进行凝胶过滤层析（Sephadex LH-20）与40％甲醇; 最后将纯化的级分重结晶，并通过高速液相色谱纯化单一活性化合物。