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公开(公告)号：KR1020120007437A
公开(公告)日：2012-01-20
申请号：KR1020110018007
申请日：2011-02-28
Applicant: 건국대학교 산학협력단
CPC classification number: C12N9/90 , C12P19/02 , C12P19/24 , C12Y503/01008
Abstract: PURPOSE: A method for preparing L-ribose using a novel mannose-6-phosphoric acid isomerase is provided to obtain the L-ribose which is used as a synthesis starting material for a pharmaceutical product. CONSTITUTION: A mannose 6-phosphoric isomerase mutant is prepared by converting from 90th amino acid, N into D. A gene encoding the mutant has a base sequence of sequence number 5. A method for preparing the mutant comprises: a step of culturing a microorganism which is transformed by an expression vector including the mannose-6-isomerase gene; and a step of isolating the isomerase from the microorganism. The ribose is produced using L-ribulose as a substrate.
Abstract translation: 目的：提供一种使用新型甘露糖-6-磷酸异构酶制备L-核糖的方法，以获得用作药物合成原料的L-核糖。 构成：通过将第90位氨基酸N转化为D来制备甘露糖6-磷酸异构酶突变体。编码突变体的基因具有序列号5的碱基序列。制备突变体的方法包括：培养微生物的步骤 其通过包含甘露糖-6-异构酶基因的表达载体转化; 以及从微生物中分离异构酶的步骤。 使用L-核酮糖作为底物产生核糖。
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公开(公告)号：KR1020100107865A
公开(公告)日：2010-10-06
申请号：KR1020090026193
申请日：2009-03-27
Applicant: 건국대학교 산학협력단
CPC classification number: C12P33/20 , C12N9/2402
Abstract: PURPOSE: A composition for preparing rear ginsenoside using beta-glycosidase and a method for preparing the ginsenoside are provided to quickly and selectively convert ginsenoside to other material. CONSTITUTION: A composition for preparing rare ginsenoside contains beta-glycosidase of high temperature. The rare ginsenosdie contains compound K, Y or Mc. The optically active temperature of the beta-glycosidase is 70-100°C. The beta-glycosidase is derived from Sulfolobus acidocaldarius. The beta-glycosidase has an amino acid of sequence number 4. The rear ginsenoside is obtained by reacting the beta-glycosidase with ginsenoside Rb1, Rb2, Rc or Rd and mixture in a reaction solvent at pH 4.5-7.0 and 70-100°C.
Abstract translation: 目的：提供一种使用β-糖苷酶制备后人参皂苷的组合物和人参皂苷的制备方法，以快速，有选择地将人参皂苷转化为其他物质。 构成：用于制备稀有人参皂甙的组合物含有高温β-糖苷酶。 罕见的人参皂甙含有化合物K，Y或Mc。 β-糖苷酶的光学活性温度为70-100℃。 β-糖苷酶衍生自酸硫酸硫杆菌（Sulfolobus acidocaldarius）。 β-糖苷酶具有序列号4的氨基酸。后人参皂苷通过β-糖苷酶与人参皂苷Rb1，Rb2，Rc或Rd和混合物在pH 4.5-7.0和70-100℃的反应溶剂中反应而获得 。
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公开(公告)号：KR1020080086085A
公开(公告)日：2008-09-25
申请号：KR1020070027751
申请日：2007-03-21
Applicant: 건국대학교 산학협력단
Abstract: A method for preparing allose by using ribose 5-phosphate isomerase is provided to improve preparation yield by inhibiting production of by-products and improve preparation specificity to allose without addition of metal ions, so that the allose prepared is useful for inhibiting growth of cancer cells and damage of internal organs caused by ischemia. A D-allose is prepared by reacting D-psicose with ribose 5-phosphate isomerase isolated from Clostridium thermocellum, which is mass produced by (1) preparing a recombinant expression vector, pQE30/ribose 5-phosphate isomerase, containing ribose 5-phosphate isomerase gene, (2) culturing a transformed microorganism, Escherichia coli BL21(DE3) pQE30/ribose 5-phosphate isomerase, (3) inducing the expression of the ribose 5-phosphate isomerase gene, and (4) separating and purifying the expressed recombinant protein.
Abstract translation: 提供通过使用核糖5-磷酸异构酶制备阿洛糖的方法，通过抑制副产物的产生来提高制备产率，并且在不添加金属离子的情况下提高对阿洛糖的制备特异性，从而制备的所述的阿洛糖可用于抑制癌细胞的生长 和由局部缺血引起的内脏损伤。 D-木糖通过将D-木糖与从热纤维梭菌分离的核糖5-磷酸异构酶反应制备，其通过（1）制备含有核糖5-磷酸异构酶的重组表达载体pQE30 /核糖-5-磷酸异构酶批量生产 基因，（2）培养转化的微生物，大肠杆菌BL21（DE3）pQE30 /核糖5-磷酸异构酶，（3）诱导核糖5-磷酸异构酶基因的表达，（4）分离纯化表达的重组蛋白 。
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公开(公告)号：KR102256624B1
公开(公告)日：2021-05-26
申请号：KR1020190031514
申请日：2019-03-19
Applicant: 주식회사 삼양사 , 건국대학교 산학협력단
Abstract: 본발명을프럭토오스-6-포스페이트(과당 6-인산)의에피머화효소단백질, 상기효소단백질을암호화하는핵산분자, 상기핵산분자를포함하는재조합벡터및 형질전환균주, 상기균주를이용한케토헥소오스생산용조성물에관한것이다.
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Patent Agency Ranking
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公开(公告)号：KR1020180127001A
公开(公告)日：2018-11-28
申请号：KR1020170062258
申请日：2017-05-19
Applicant: 건국대학교 산학협력단
IPC: C12P7/64 , C12P17/02 , C12N9/02 , C12N9/14 , C07C59/105 , C07D303/04
Abstract: 본발명은서열번호 1로기재되는아미노산서열로이루어진 12-지방산화효소; 또는상기 12-지방산화효소및 서열번호 2로기재되는아미노산서열로이루어진에폭사이드가수분해효소를포함하는수산화지방산, 헤폭실린 B 또는트리오실린 B 제조용조성물; 및이를이용한수산화지방산, 헤폭실린 B 또는트리오실린 B 제조방법에관한것이다.
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公开(公告)号：KR101383591B1
公开(公告)日：2014-04-10
申请号：KR1020110061911
申请日：2011-06-24
Applicant: 건국대학교 산학협력단 , 이화여자대학교 산학협력단
Abstract: 본 발명은 10-하이드록시스테아르 산 (10-Hydroxystearic acid)의 생산방법에 관한 것으로, 더욱 상세하게는 리시니바실러스 후시포르미스 (
lysinibacillus
fusiformis), 마크로코코스 카제리티쿠스 (
Macrococcus
caseolyticus
), 프로피오니박테리움 아크네스 (
Propionibacterium
acnes
), 스테노트로포모나스 말토필리아 (
Stenotrophomonas
maltophilia
) 균 유래의 올레산 수화효소 (Oleate hydratase) 및 유전자를 포함하는 재조합 발현 벡터, 이로 형질전환 된 미생물을 이용하여 올레산 (oleate) 를 반응시켜 10-하이드록시스테아르 산을 고수율로 생산하는 방법이다.
본 발명에 따르면, 높은 특이성과 친환경적인 방법으로 산업품의 원료 물질인 10-하이드록시스테아르 산을 높은 수율로 생산할 수 있으며, 이렇게 생산된 10-하이드록시스테아르 산은 락톤(Lactone) 의 합성 시작물질로서 유용하게 사용될 수 있다.
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