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公开(公告)号：KR1020150063338A
公开(公告)日：2015-06-09
申请号：KR1020150069097
申请日：2015-05-18
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은배추병저항성연관 SNP 프라이머쌍 7,645개의활용성을확대하고자, 배추의병저항성과뿌리특성을동시에평가할수 있는 SNP 분자마커를개발하고, 이의용도를제공하고자하는것이다.
Abstract translation: 本发明的目的是开发一种能够同时评估大白菜的抗病性和根性状的SNP分子标记，以扩大与大白菜抗病性相关的7,645个SNP引物对的利用，并提供 使用它。
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Patent Agency Ranking
公开(公告)号：KR1020140126207A
公开(公告)日：2014-10-30
申请号：KR1020130044484
申请日：2013-04-22
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6895 , C12Q2600/156
Abstract: 본 발명은 배추의 병저항성 연관 SNP 프라이머를 타종인 양배추에의 적용가능성을 검토하고, 양배추 뿌리혹병 저항성을 효율적으로 평가할 수 있는 SNP 분자마커 및 이의 용도를 제공하는 것이다.
Abstract translation: 在本发明中，提供了单核苷酸多态性（SNP）分子标记及其用途，能够有效地检测与白菜病害相关的SNP引物是否可应用于其他类型的白菜的SNP分子标记 并评估对根茎（CR）疾病的抵抗力。 更具体地，提供了一种筛选具有抗CR性的白菜的方法，包括筛选具有耐CR性的白菜类型并从白菜分离基因组DNA的步骤。
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公开(公告)号：KR1020140126114A
公开(公告)日：2014-10-30
申请号：KR1020130044240
申请日：2013-04-22
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6844 , C12Q1/6888 , C12Q2600/156 , C12Q2600/16
Abstract: 본 발명은 배추 병저항성 연관 SNP 프라이머쌍 7,645개의 활용성을 확대하고자, 배추의 병저항성과 뿌리 관련 특성을 동시에 평가할 수 있는 SNP 분자마커를 개발하고, 이의 용도를 제공하고자 하는 것이다.
Abstract translation: 在本发明中，提供了用于评价中国白菜的抗病性和根特性的SNP分子标记物的开发，以及使用该标记来扩大与白菜病害相关的7,645个SNP引物对的可用性。 更具体地说，提供了一种用于同时评估大白菜的抗病性和根特性的试剂盒。
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公开(公告)号：KR1020140119518A
公开(公告)日：2014-10-10
申请号：KR1020130035303
申请日：2013-04-01
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6844 , C12Q1/6895 , C12Q2600/156
Abstract: The present invention aims to: develop an SNP molecular marker that can be used for analyzing diseases resistance and leaf traits of a Chinese cabbage at the same time so as to incases applicability of 7,645 pairs of a SNP primer associated with disease resistance of the Chinese cabbage; and provide use thereof. The present invention comprises the steps of: separating genome DNAs of the Chines cabbage; performing a polymerase chain reaction (PCR) using a set of the primer; and identifying products of the PCR.
Abstract translation: 本发明旨在开发可同时用于分析大白菜病害抗性和叶片性状的SNP分子标记，从而适用7,645对与大白菜抗病性相关的SNP引物 ; 并提供其使用。 本发明包括以下步骤：分离中国白菜的基因组DNA; 使用一组引物进行聚合酶链反应（PCR）; 并鉴定PCR的产物。
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公开(公告)号：KR100344573B1
公开(公告)日：2002-08-19
申请号：KR1019990007458
申请日：1999-03-06
Applicant: 대한민국(농촌진흥청장)
IPC: C12N5/04
Abstract: 본발명은국내에서재배되고있는고추재배종및 재래종을재료로하고아그로박테리움을형질전환매개체로이용한효율적인유전자전환기법에관한것이다. 고추형질전환은국내에서도일부연구자들이시도하였으나슈트(shoot)에서뿌리(root)를유도하는과정에서재현성이낮고효율도지극히낮은단점이있으나본 방법은이 단계의한계를극복하여뿌리유도율이 25%에달하고그 기간도 3주이내에뿌리를유도시킬수 있는기법이기때문에현재까지시도된고추형질전환방법중에서가장우수한것이다. 또한형질전환고추를개발하는데소요되는기간을 4개월내외로현저히단축시키며앞으로고추에유용한유전자를형질전환시켜국내농업발전과종자산업을보호하는데기여할수 있으리라사료되며학문적으로도원예작물형질전환의획기적전기로평가될수 있으리라본다.
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公开(公告)号：KR1020000059676A
公开(公告)日：2000-10-05
申请号：KR1019990007458
申请日：1999-03-06
Applicant: 대한민국(농촌진흥청장)
IPC: C12N5/04
CPC classification number: C12N15/8205 , C12N5/04 , C12N15/8227
Abstract: PURPOSE: Provided is a method for preparing transformed red pepper using Agrobacterium tumefaciens LBA4404, which enables to introduce useful genes into crops. CONSTITUTION: A transformed red pepper is prepared by the following steps of: transforming red pepper cotyledon by using of Agrobacterium tumefaciens LBA4404; inducing shoot only from the transformed cotyledon tissue by cultivation in shoot-induction medium, which comprises MS(Murashige & Skoog) base medium including 3% of sucrose, 0.4% of gelrite, zeatin and indole acetic acid (IAA) as hormone, kanamycin and carbenicillin as antibiotics; cutting and serial passage culturing the shoot in shoot-elongation medium, which comprises MS(Murashige & Skoog) base medium including 3% of sucrose, 0.4% of gelrite, zeatin and NAA as hormone, kanamycin and carbenicillin as antibiotics to differentiate young plant; developing root by cultivation of the differentiated young transformant in root-induction medium, which comprises MS(Murashige & Skoog) base medium including 3% of sucrose, 0.4% of gelrite, IBA as hormone, kanamycin and carbenicillin as antibiotics.
Abstract translation: 目的：提供使用根癌土壤杆菌LBA4404制备转化的红辣椒的方法，其能够将有用的基因引入作物。 构成：通过以下步骤制备转化的红辣椒：通过使用根癌土壤杆菌LBA4404转化红辣椒子叶; 通过培养在诱导培养基中诱导来自转化的子叶组织的芽，其包括含有3％蔗糖，0.4％的gelrite，玉米素和吲哚乙酸（IAA）作为激素的MS（Murashige＆Skoog）基培养基，卡那霉素和 羧苄青霉素作为抗生素; 切片和连续传代在芽延伸培养基中培养芽，其中包括含有3％蔗糖，0.4％gelrite，zeatin和NAA作为激素的MS（Murashige＆Skoog）基培养基，卡那霉素和羧苄青霉素作为抗生素以分化幼苗; 通过在根诱导培养基中培养分化的幼年转化子来培养根，其包括含有3％蔗糖，0.4％的gelrite，IBA作为激素，卡那霉素和羧苄青霉素作为抗生素的MS（Murashige＆Skoog）基培养基。
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