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公开(公告)号：KR1020110093164A
公开(公告)日：2011-08-18
申请号：KR1020100013042
申请日：2010-02-11
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8205 , A01H4/001 , A01H4/005 , C12N5/0025 , C12N15/8216 , C12N15/8241
Abstract: PURPOSE: A method for enhancing transformation efficiency of Brassica campestris L. using Agrobacterium is provided to establish stable transformation system of high efficiency. CONSTITUTION: A method for enhancing transformation efficiency of Brassica campestris L. comprises: a step of performing dark germination of Brassica campestris L. seeds at 24-26°C for 3-5 days; a step of culturing the hypocotyl part of Brassica campestris L. in a MS medium containing 0.5-1.5 mg/L of NAA and 3-5 mg/L of BA at 22-24°C for 1-3 days by photo-cultivation; a step of suspending Agrobacterium tumefaciens containing a recombinanat plant expression vector to a medium; a step of dipping the hypocotyl part of Brassica campestris L. in the prepared strain-inoculated medium for 5-15 minutes; a step of culturing the hypocotyl part of Brassica campestris L. in a medium I containing NAA, BA, AgNO_3, and antibiotics for 2-3 months to induce a shoot; and a step of culturing the shoot in a medium II.
Abstract translation: 目的：提供使用农杆菌提高芥菜转化效率的方法，建立高效率的稳定转化体系。 构成：提高芥菜转化效率的方法包括：在24-26℃下进行芸苔属种子黑萌发3-5天的步骤; 在含有0.5-1.5mg / L的NAA和3-5mg / L BA的MS培养基中培养芥菜的下胚轴部分在22-24℃下培养1-3天的步骤; 将含有重组酶植物表达载体的根癌农杆菌悬浮于培养基中的步骤; 将芥菜的下胚轴部分浸渍在制备的菌株接种培养基中5-15分钟的步骤; 在含有NAA，BA，AgNO_3和抗生素的培养基I中培养芥菜的下胚轴2-3个月以诱导芽的步骤; 并在培养基II培养苗条的步骤。
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公开(公告)号：KR1020090057852A
公开(公告)日：2009-06-08
申请号：KR1020070124614
申请日：2007-12-03
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8274 , A01H4/005 , A01H4/008 , C12N5/0025 , C12N15/8205 , C12N15/8279 , C12N2500/30
Abstract: A method for manufacturing a herbicide resistance garlic is provided to transform the herbicide resistance gene in a garlic using an agrobacterium. A method for manufacturing a herbicide resistance garlic comprises: a step of adding 0.5-1.5mg/L of 2,4-D(2,4-dichlorophenoxyacetic acid) and 0.05-0.15mg/L of IAA(indoleacetic acid) in MS broth and inducing a garlic callus for seven to nine weeks; a step of co-culturing an Agrobacterium tumefaciens with the induced garlic callus and transforming; a step of selecting plant cell in antibiotics added selection medium for 11 to 13 weeks; and a step of redifferentiating the selected garlic cell.
Abstract translation: 提供了一种制造抗除草剂大蒜的方法，以使用农杆菌转化大蒜中的除草剂抗性基因。 一种抗除草剂大蒜的制造方法，其特征在于，在MS肉汤中加入0.5-1.5mg / L的2,4-D（2,4-二氯苯氧基乙酸）和0.05-0.15mg / L的IAA（吲哚乙酸） 并诱导大蒜愈伤组织七至九周; 与诱导的大蒜愈伤组织共培养根癌土壤杆菌并转化的步骤; 选择植物细胞的抗生素添加选择培养基11〜13周的步骤; 以及重新分配所选择的大蒜细胞的步骤。
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Patent Agency Ranking
公开(公告)号：KR1020140078389A
公开(公告)日：2014-06-25
申请号：KR1020120147692
申请日：2012-12-17
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a new variety of garlic having an improved anticancer activity by cross-breeding a fertile garlic, and the new variety of garlic has an anticancer activity and strong properties against viral disease and garlic leaf blight compared to an existing cultivated variety. Thus the present invention provides a new variety of garlic having a more improved anticancer activity than an existing garlic variety, and can be applied to new food and drug not only in Korea where a garlic is used as a seasoning, but also in a foreign country.
Abstract translation: 本发明涉及一种通过杂交繁殖大蒜具有改善的抗癌活性的新品种，并且与现有栽培品种相比，新品种大蒜具有抗病毒活性和抗病毒性疾病和大蒜叶枯病的强烈性能。 因此，本发明提供了一种具有比现有大蒜品种更好的抗癌活性的新品种，并且可以应用于新鲜食品和药物，不仅在韩国使用大蒜作为调味品，而且在国外 。
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公开(公告)号：KR101174491B1
公开(公告)日：2012-08-22
申请号：KR1020100013042
申请日：2010-02-11
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은아그로박테리움을이용한배추의형질전환효율을증가시키는방법및 상기방법으로제조된배추식물체에관한것으로, 공동배양시현탁처리방법, 배추하배축절편으로부터캘러스유기및 재분화, 및재분화시배양온도처리를달리하여배추의형질전환효율을증가시키는방법및 상기방법으로제조된고효율의형질전환배추식물체및 이의종자에관한것이다.
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公开(公告)号：KR1020090085725A
公开(公告)日：2009-08-10
申请号：KR1020080011467
申请日：2008-02-05
Applicant: 대한민국(농촌진흥청장)
Abstract: A method for discriminating Plasmodiophora brassicae in a real time is provided to quickly detect the Plasmodiophora brassicae race. A method for discriminating Plasmodiophora brassicae race comprises: a step of germinating host plant and removing soil; a step of dipping the host plant in spore suspension to induce infection; and a step of discriminating Plasmodiophora brassicae race. A liquid for water culture comprises 708mg of KNO3, 590mg of Ca(NO3)2À4H2O, 246mg of MgSO4À7H2O, 115 mg of NH4H2PO4, 20mg of Fe-EDTA, 3mg of H3BO3, 2mg of MnSO4À4H2O, 0.22mg of ZnSO4À7H2O, 0.05mg of CuSO4À5H2O and 0.02mg of Na2MoO4À2H2O.
Abstract translation: 提供了一种实时鉴别疟原虫的方法，用于快速检测豌豆豌豆种质。 鉴别疟原虫种的方法包括：萌发宿主植物并去除土壤的步骤; 将宿主植物浸泡在孢子悬浮液中以诱导感染的步骤; 以及歧视疟原虫种族的一个步骤。 用于水培养的液体包括708mg KNO 3，590mg Ca（NO 3）2·4H 2 O，246mg MgSO 4·7H 2 O，115mg NH 4 H 2 PO 4，20mg Fe-EDTA，3mg H 3 BO 3，2mg MnSO 4·4H 2 O，0.22mg ZnSO 4·7H 2 O，0.05mg CuSO 4·5H 2 O 和0.02mg Na2MoO4·2H2O。
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公开(公告)号：KR1020140126207A
公开(公告)日：2014-10-30
申请号：KR1020130044484
申请日：2013-04-22
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6895 , C12Q2600/156
Abstract: 본 발명은 배추의 병저항성 연관 SNP 프라이머를 타종인 양배추에의 적용가능성을 검토하고, 양배추 뿌리혹병 저항성을 효율적으로 평가할 수 있는 SNP 분자마커 및 이의 용도를 제공하는 것이다.
Abstract translation: 在本发明中，提供了单核苷酸多态性（SNP）分子标记及其用途，能够有效地检测与白菜病害相关的SNP引物是否可应用于其他类型的白菜的SNP分子标记 并评估对根茎（CR）疾病的抵抗力。 更具体地，提供了一种筛选具有抗CR性的白菜的方法，包括筛选具有耐CR性的白菜类型并从白菜分离基因组DNA的步骤。
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公开(公告)号：KR100960886B1
公开(公告)日：2010-06-01
申请号：KR1020070124614
申请日：2007-12-03
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은
제초제 저항성 마늘의 제조 방법 및 그에 따른 제초제 저항성 마늘에 관한 것으로, 더욱 상세하게는 (1) MS 배지에
0.5~1.5 mg/L의 2,4-D(2,4-dichlorophenoxyacetic acid)와 0.2~0.3mg/L의 IAA(indoleacetic acid)를 첨가하여 7~9주간 마늘 캘러스를 유도하는 단계; (2) 상기 유도된 마늘 캘러스에 제초제 저항성 유전자가 삽입된 식물 발현 벡터를 포함하는 아그로박테리움 튜머파시엔스
(Agrobacterium tumefaciens) 를 공동배양 배지에서 공동배양하여 형질전환시키는 단계; (3) 상기 공동배양한 식물세포를 항생제가 첨가된 선발 배지에서 11~13주간 선발하는 단계; 및 (4) 상기 선발된 마늘 세포를 재분화 배지에서 재분화하는 단계를 포함하는 제초제 저항성 마늘의 제조 방법과 상기 방법에 따른 제초제 저항성 마늘 식물체에 관한 것이다.
마늘, 아그로박테리움, 형질전환, 방법, 제초제, 저항성
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