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公开(公告)号：KR101594988B1
公开(公告)日：2016-02-19
申请号：KR1020150131647
申请日：2015-09-17
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은피마자(L.)로부터분리된 RcLPAT376 유전자및 이를이용한형질전환한식물체의하이드록시지방산, 구체적으로 12-하이드록시-옥타데카-9-에노인산(12-hydroxy-octadeca-9-enoic acid)의생산증진에관한것으로, 상기 RcLPAT376 유전자로형질전환한형질전환식물체의종자에서하이드록시지방산의함량이향상되었으므로상기유전자를이용하여다양한식물체에서하이드록시지방산의생산을증가시키는데유용하게이용할수 있다.
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公开(公告)号：KR1020150136672A
公开(公告)日：2015-12-08
申请号：KR1020140063649
申请日：2014-05-27
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은종자발달을조절하는유전자와노화특이프로모터를포함하는재조합벡터및 이를이용한형질전환식물체제조방법에관한것으로, 본발명에따른재조합벡터는식물생장과정중 잎이노화될수록발현량이증가됨으로써식물의생장에는영향을주지않으면서잎에서중성지방함량이증대된식물을제조할수 있다.
Abstract translation: 本发明涉及包含调控种子发育的基因和老化特异性启动子的重组载体，以及使用其的转基因植物的制备方法。 根据本发明，重组载体的表达水平随植物生长过程中叶变老而增加。 因此，可以生产叶片中甘油三酯含量增加而对植物生长没有任何影响的植物。
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公开(公告)号：KR1020150104554A
公开(公告)日：2015-09-15
申请号：KR1020150126030
申请日：2015-09-07
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/82 , C07K14/415 , A01H5/00
Abstract: 본 발명은 피마자(
Ricinus
communis
L.)로부터 분리된 RcLPAT299 유전자 및 이를 이용한 형질전환한 식물체의 리시놀레인 지방산 생산 증진에 관한 것으로, 상기 RcLPAT299 유전자로 형질전환한 형질전환 식물체의 종자에서 리시놀레인 지방산의 함량이 향상되었으므로 상기 유전자를 이용하여 다양한 식물체에서 리시놀레인 지방산의 생산을 증가시키는데 유용하게 이용할 수 있다.Abstract translation: 本发明涉及从蓖麻（Ricinus communis L.）分离的蓖麻豆溶血磷脂酰基转移酶299（RcLPAT299）基因，并且通过使用该转基因植物转化的转基因植物的蓖麻油酸产量增加，其中该基因可广泛用于增加 由于RcLPAT299基因转化的转基因植物的种子中蓖麻油酸的含量增加，因此各种植物中的蓖麻油酸含量增加。
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公开(公告)号：KR1020150052904A
公开(公告)日：2015-05-15
申请号：KR1020130134133
申请日：2013-11-06
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은고도불포화지방산생합성에관한것으로, 보다상세하게는 pAO815 벡터를이용한세 개의유전자와,가동시에발현되도록다중발현벡터를만들어진핵세포의단백질의생산에이용되는유용한시스템인메탄이용효모인에형질전환함으로써효모가생산해내는기질 linoleic acid (LA, C18:2 n-6) 또는 a-linolenic acid (ALA, C18:3 n-3)로부터고도불포화지방산인, gamma-linolenic acid (GLA, C18:3 n-6), stearidonic acid (STA, C18:4 n-3), di-homo-linolenic acid (DGLA, 20:3n-6), eicosatetraenoic acid (ETA, 20:4n-3), arachidonic acid (ARA; 20:4n-6), eicosapentaenoic acid (EPA, 20:5n-3), docosatetraenoic acid (DTA, 22:4n-6) 또는 docosapentaenoic acid (DPA, 222:5n-3)로의전환을가스크로마토그래피로분석하여 3개유전자의동시발현기능을검정하였으므로, 이를고도불포화지방산생산자원으로유용하게사용할수 있다.
Abstract translation: 本发明涉及多不饱和脂肪酸的生物合成，更具体地说，使用pAO815载体共同表达McD6DES，AsELOVL5和PtD5DES的三个基因的多重表达载体，并将载体转化为巴斯德毕赤酵母，其使用甲烷为酵母 用于生产真核细胞蛋白质的可用系统，由于分析了由诸如亚油酸（LA，C18：2 n-6）的酵母产生的细胞外基质的转化，有价值地用作多不饱和脂肪酸生产源， 或α-亚麻酸（ALA，C18：3n-3）与多亚不饱和脂肪酸如γ-亚麻酸（GLA，C18：3n-6），十八碳四烯酸（STA，C18：4n-3） 二 - 十二碳烯酸（DGLA，20：3n-6），二十碳四烯酸（ETA，20：4n-3），花生四烯酸（ARA; 20：4n-6），二十碳五烯酸 ），二十二碳四烯酸（DTA，22：4n-6）或二十二碳五烯酸（DPA，222：5n-3），通过气相色谱法检测共表达 功能的三个基因。
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公开(公告)号：KR1020140049853A
公开(公告)日：2014-04-28
申请号：KR1020120116185
申请日：2012-10-18
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/825 , C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8257 , C12N2310/14
Abstract: The present invention relates to a method for controlling the carotenoid amount of plants using the control of the expression of PAP8 (Plastid lipid-associated protein-8) genes. More specifically, the PAP-8 genes, especially PAP8A and PAP8B genes are confirmed to be existed in chlorophyll of the plants, have a function related to the synthesis and accumulation of carotenoid, have a function of controlling the carotenoid amount of the plants through the increase or control of the expression of the PAP-8 genes in a plant body. By using the expression control of genes which shows homogeny in the PAP-8 genes, and a base sequence and a protein sequence thereof, the carotenoid production in various kinds of plant bodies can be improved.
Abstract translation: 本发明涉及使用对PAP8（质体脂质相关蛋白-8）基因表达的控制来控制植物的类胡萝卜素含量的方法。 更具体地说，PAP-8基因，特别是PAP8A和PAP8B基因被证实存在于植物的叶绿素中，具有与类胡萝卜素的合成和积累相关的功能，具有通过植物控制植物的类胡萝卜素含量的功能 增加或控制植物体内PAP-8基因的表达。 通过使用在PAP-8基因中显示均匀的基因的表达控制及其碱基序列和蛋白质序列，可以提高各种植物体中的类胡萝卜素生产。
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Patent Agency Ranking
公开(公告)号：KR101303697B1
公开(公告)日：2013-09-09
申请号：KR1020110072397
申请日：2011-07-21
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 피마자의 사이토크롬(cytochrome) B5에서 분리한 유전자(CYB5-1 내지 CYB5-7)의 식물의 하이드록시 지방산 생산 촉진 용도에 관한 것으로서, 상기 유전자를 식물체 내로 도입하여 세포내에서 발현시킴으로써, 식물의 하이드록시 지방산 생산을 촉진시킬 수 있다. 보다 구체적으로 본 발명은 피마자에서 분리한 유전자의 하이드록시 지방산 생산 촉진 용도, 상기 유전자가 도입된 형질전환 식물체 및 상기 유전자를 식물체에 도입시켜 식물의 하이드록시 지방산 생산을 촉진시키는 방법에 관한 것이다.
본 발명에 따른 유전자는 하이드록시 지방산을 생산하는 형질전환 식물 개발에 유용하게 사용될 수 있어, 하이드록시 지방산이 사용되는 윤활유, 페인트, 섬유소재, 의약 등의 분야에 기여할 수 있다.-
公开(公告)号：KR1020130011325A
公开(公告)日：2013-01-30
申请号：KR1020110072397
申请日：2011-07-21
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A gene(CYB5-1 to CYB5-7) isolated from castor cytochrome B5 is provided to promote hydroxyl fatty acid production in a plant and to develop a transgenic plant which produces hydroxyl fatty acid. CONSTITUTION: A castor cytochrome B5 polypeptide has an amino acid sequence of sequence number 2, 4, 6, 8, 10, 12, or 14. A castor cytochrome B5 gene encoding the polypeptide has a nucleic acid sequence of sequence number 1, 3, 5, 7, 9, 11, or 13. The castor cytochrome B5 gene is derived from castor. A composition for promoting hydroxyl fatty acid production in a plant contains the polypeptide or the gene. A method for promoting hydroxyl fatty acid production in the plant comprises a step of transducing the gene into a plant by transforming the plant with a vector containing the gene.
Abstract translation: 目的：提供从蓖麻细胞色素B5分离的基因（CYB5-1至CYB5-7），以促进植物中的羟基脂肪酸产生并开发产生羟基脂肪酸的转基因植物。 构成：蓖麻细胞色素B5多肽具有序列号2,4,6,8,10,12或14的氨基酸序列。编码多肽的蓖麻细胞色素B5基因具有序列号1,3， 5，7，9，11或13.脚轮细胞色素B5基因衍生自蓖麻。 用于促进植物中羟基脂肪酸产生的组合物含有多肽或基因。 用于促进植物中羟基脂肪酸产生的方法包括通过用含有该基因的载体转化植物将基因转导到植物中的步骤。
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公开(公告)号：KR1020120002566A
公开(公告)日：2012-01-06
申请号：KR1020110139449
申请日：2011-12-21
Applicant: 대한민국(농촌진흥청장)
IPC: C12N9/10 , C07K14/415 , A01H5/00 , C12N15/54
Abstract: PURPOSE: A Ricinus communis L.-derived protein with PDAT(phospholipid diacylglycerol acyltransferase) is provided to enhance production of hydroxyl fatty acid. CONSTITUTION: A protein with PDAT(phospholipid diacylglycerol acyltransferase) activity has an amino acid of sequence number 3. The PDAT protein is derived from Ricinus communis L. A nucleic acid molecule encoding the PDAT protein has a base sequence of sequence number 6. A transgenic plant with enhanced hydroxyl fatty acid production is prepared by transforming the nucleic acid molecule with a recombinant vector. A method for preparing the hydroxyl fatty acid comprises: a step of culturing the transgenic plant; and a step of collecting hydroxyl fatty acid from seeds of the transgenic plant.
Abstract translation: 目的：提供具有PDAT（磷脂二酰基甘油酰基转移酶）的蓖麻L.衍生蛋白，以增强羟基脂肪酸的产生。 构成：具有PDAT（磷脂二酰基甘油酰基转移酶）活性的蛋白质具有序列号3的氨基酸.PDAT蛋白源自蓖麻（Ricinus communis L.）。编码PDAT蛋白的核酸分子具有序列号6的碱基序列。转基因 通过用重组载体转化核酸分子制备具有增强的羟基脂肪酸生产的植物。 一种制备羟基脂肪酸的方法包括：培养转基因植物的步骤; 以及从转基因植物的种子中收集羟基脂肪酸的步骤。
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公开(公告)号：KR1020120002565A
公开(公告)日：2012-01-06
申请号：KR1020110139448
申请日：2011-12-21
Applicant: 대한민국(농촌진흥청장)
IPC: C12N9/10 , C07K14/415 , A01H5/00 , C12N15/54
Abstract: PURPOSE: A novel Ricinus communis L.-derived protein with PDAT(phospholipid diacylglycerol acyltransferase) activity is provided to be applied to various industries. CONSTITUTION: A protein with PDAT(phosphlipid diacylglycerol acyltransferase) activity has an amino acid sequence of sequence number 1. The protein is derived from Ricinus communis L. A nucleic acid molecule encoding the protein has a base sequence of sequence number 4. A recombinant vector contains the nucleic acid molecule. A transgenic plant contains a gene of hydroxyl fatty acid synthesis. The gene encodes FAG(fatty acid hydroxylase). A method for preparing hydroxyl fatty acid comprises: a step of culturing the transgenic plant; and a step of collecting hydroxyl fatty acid from seeds of the transgenic plant.
Abstract translation: 目的：提供一种具有PDAT（磷脂二酰基甘油酰基转移酶）活性的新型蓖麻蓖麻L.衍生蛋白，适用于各行业。 构成：具有PDAT（磷脂二酰基甘油酰基转移酶）活性的蛋白质具有序列号1的氨基酸序列。该蛋白质来自蓖麻（Ricinus communis L）。编码蛋白质的核酸分子具有序列号4的碱基序列。重组载体 含有核酸分子。 转基因植物含有羟基脂肪酸合成基因。 该基因编码FAG（脂肪酸羟化酶）。 一种制备羟基脂肪酸的方法包括：培养转基因植物的步骤; 以及从转基因植物的种子中收集羟基脂肪酸的步骤。
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公开(公告)号：KR1020110123132A
公开(公告)日：2011-11-14
申请号：KR1020100042607
申请日：2010-05-06
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/1029 , C12N15/8247 , C12Y111/02004 , C12Y203/01158
Abstract: PURPOSE: A novel protein derived from Ricinus communis L. with phospholipids diacylglycerol acyltransferase(PDAT) activity is provided to ensure functionality for various industrial applications. CONSTITUTION: A protein having phospholipid diacylglycerol acyltransferase(PDAT) has an amino acid sequence of sequence number 1, 2, or 3. The protein is derived from Ricinus communis L. A nucleic acid molecule encoding the protein has a base sequence of sequence number 4, 5, or 6. A recombinant vector contains the nucleic acid molecules. A method for producing hydroxy fatty acid comprises: a step of culturing transgenic plants; and a step of collecting hydroxy fatty acid from the seeds of the transgenic plants.
Abstract translation: 目的：提供一种源自蓖麻（Ricinus communis L.）的新型蛋白质，具有磷脂二酰基甘油酰基转移酶（PDAT）活性，以确保各种工业应用的功能。 构成：具有磷脂二酰基甘油酰基转移酶（PDAT）的蛋白质具有序列号1,2或3的氨基酸序列。该蛋白质来自蓖麻（Ricinus communis L）。编码该蛋白质的核酸分子具有序列号4的碱基序列 ，5或6.重组载体含有核酸分子。 一种生产羟基脂肪酸的方法包括：培养转基因植物的步骤; 以及从转基因植物的种子中收集羟基脂肪酸的步骤。
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