Abstract:
본 발명은 갯장어 유래의 지방산 사슬연장효소 단백질 및 이를 이용하여, 디-호모-감마리놀레익산(di-homo-gamma linolenic acid, DHLA), 도코사테트라에노익산(docosatetraenoic acid, DTA) 및 도코사펜타에노익산(docosapentaenoic acid, DPA)로 이루어진 군으로부터 선택되는 불포화지방산을 생산하는 방법에 관한 것이다.
Abstract:
A MYB60 gene is provided to inhibit biosynthesis of anthocyanin, so that it is useful for change of flower color of flower plants and for development of new varieties of flower plants. A transgenic lettuce containing the same MYB60 gene is also provided to verify anthocyanin biosynthesis-inhibiting effects of the gene through phenotype by using a plant system. A MYB60 gene inhibiting biosynthesis of anthocyanin has the nucleotide sequence of SEQ ID NO:1. A vector 22103-MYB60 contains the MYB60 gene. A transformed Agrobacterium tumefaciens EHA105(KACC 95045P) is produced with the vector 22103-MYB60. A method for preparing a transgenic lettuce comprises the steps of: (a) preparing an expression vector containing the MYB60 gene and a basta resistant bar gene as a selection marker; (b) producing Agrobacterium tumefaciens EHA105(KACC 95045P) by transforming with the expression vector; (c) co-culturing cotyledon of a lettuce plant with the transformed Agrobacterium tumefaciens EHA105(KACC 95045P); (d) selecting shoots of lettuce plant from a selection medium and inducing plants from the shoots in plant-inducing medium; and (e) transplanting the induced lettuce plants to an in vitro medium and the soil to acclimate them.
Abstract:
본 발명은 녹차 어린잎 유래 델타-6 불포화효소 유전자, 이 유전자를 포함하는 발현벡터, 이 발현벡터로 형질전환된 형질전환체 및 이 형질전환체의 제조방법에 관한 것으로서, 더욱 상세하게는, 서열번호 1로 기재되는 염기서열로 이루어지는, 녹차 어린잎 유래 델타-6 불포화효소를 코딩하는 유전자, 이 유전자를 포함하는 발현벡터 pYES2/CT-D6DES, 이 발현벡터로 형질전환된 효모( Saccharomyces cerevisiae ) 형질전환체(수탁번호: KACC 93021) 및 이 형질전환체의 제조방법에 관한 것이다. 델타-6 불포화효소, pYES2/CT, Saccharomyces cerevisiae
Abstract translation:本发明是绿茶叶得到的小增量-6不饱和基因,涉及一种表达载体,制造方法的转化,并用含有该基因的表达载体转化材料转化的转化体,更具体地,SEQ ID NO: 由以第所示的碱基序列的1,转化的酵母到编码酶的来自茶的嫩叶增量-6基因不饱和的,表达包含基因载体pYES2 / CT-D6DES,表达载体(
Abstract:
본 발명은 신규한 갯장어 유래 델타-6 불포화효소 유전자, 이 유전자를 포함하는 발현벡터, 이 발현벡터로 형질전환된 형질전환체 및 이 형질전환체의 제조방법에 관한 것이다. 본 발명의 갯장어 유래 델타-6 불포화효소 유전자를 분리하여 그 기능을 확인하고, 리놀레산을 함유하는 들깨 등 유지작물에 이용할 경우, 고부가의 기능성 감마-리놀렌산을 생산하는 유지작물을 제공할 수 있고, 또한 EPA 또는 DHA과 같은 오메가-3의 고도불포화지방산을 생성하기 위한 대사공학기술에 적용될 수 있다.
Abstract:
PURPOSE: An RNAi carrier containing cacatus virus X coat protein gene is provided to product transformed cactus having CVX resistance. CONSTITUTION: A cacatus virus X resistant plasmid(KACC 95089P) contains cacatus virus X coat protein gene of sequence number 1 in a forward direction and reverse direction. An Agrobacterium transformant is prepared using the cacatus virus X resistant plasmid. A method for producing the transformed cactus comprises: a preparing the plasmid to which the cacatus virus X coat protein gene; a step of transforming Agrobacterium using the plasmid; and a step of transforming cactus using the Agrobacterium.
Abstract:
A fusion protein of Escherichia coli enterotoxin protein and a neuraminidase epitope of Newcastle disease virus is provided to increase immunity against Newcastle disease virus in poultry, so that the protein is useful for prevention of Newcastle disease virus. A fusion protein of E. coli heat-labile enterotoxin subunit B and a neuraminidase epitope of Newcastle disease virus has the amino acid sequence of SEQ ID NO:1. A polynucleotide encoding the fusion protein has the nucleotide sequence of SEQ ID NO:2. An expression vector pLD-CtV-HN contains the polynucleotide of SEQ ID NO:2. A vaccine composition for preventing Newcastle disease virus contains the fusion protein as an effective ingredient. The immunity against Newcastle disease virus in poultry is increased by administering the fusion protein or a transgenic plant containing the expression vector pLD-CtV-HN into poultry.
Abstract translation:提供了大肠杆菌肠毒素蛋白和新城疫病毒的神经氨酸酶表位的融合蛋白,以提高家禽对新城疫病毒的免疫力,从而使该蛋白可用于预防新城疫病毒。 大肠杆菌不耐热肠毒素亚基B和新城疫病毒的神经氨酸酶表位的融合蛋白具有SEQ ID NO:1的氨基酸序列。 编码融合蛋白的多核苷酸具有SEQ ID NO:2的核苷酸序列。 表达载体pLD-CtV-HN含有SEQ ID NO:2的多核苷酸。 用于预防新城疫病毒的疫苗组合物含有融合蛋白作为有效成分。 通过向家禽施用融合蛋白或含有表达载体pLD-CtV-HN的转基因植物来增加家禽对新城疫病毒的免疫力。
Abstract:
PURPOSE: An antiviral protein CAP30 separated from Chenopodium album var. is provided, which can be used as antiviral bioagricultural drugs or human antiviral medicines. CONSTITUTION: The antiviral protein CAP30 having 30,000 dalton of molecular weight is isolated by the steps of: grinding the leaves of Chenopodium album var. and extracting it with a buffer solution A containing 50 mM of sodium chloride, 0.2 mM of PMSF, 20 mM of Tris-HCl, 1mM of EDTA, and 10 mM of beta-mercaptoethanol; filtering and centrifuging the extract and collecting the supernatant; adding 30 to 80% of ammonium sulfate and dialyzing it with the buffer solution A; passing the concentrate through the DE-52 cellulose column and subjecting it to linear gradient of buffer solution A to separate the active fragments; and purifying the active fragments by subjecting it to chromatography. The gene encoding the antiviral protein CAP30 is sequenced and its nucleotide sequence is represented by sequence ID No. 1.